I-Corps: Field-deployable Diagnostic Laboratory for the Food Industry

I-Corps：食品工业现场部署诊断实验室

• 批准号: 2034362
• 负责人: Euiwon Bae
• 金额: $ 5万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-15 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2034362&HistoricalAwards=false
• 关键词: Corps; Field; deployable; Diagnostic; Laboratory

The broader impact/commercial potential of this I-Corps project is the development of a field-deployable, food safety assessment platform. Current protocols require that food samples be shipped and further analyzed in a central laboratory. Food products under testing will have already been sold and consumed by the customer. The aims of this effort are to provide the inspection results within 10-12 hrs so the manufacturers have timely feedback, and to enable database options to digitally manage the inspection results and records so that this information could be potentially integrated into the upcoming block chain revolution in the food safety area. Success will provide earlier time-to-detection of food pathogens so that regulatory agencies can implement more rapid countermeasures to potential outbreak situations. For manufacturers, the goal is to provide timely test to results so that manufacturers themselves may conduct their own inspections and find the vulnerable spots for contamination. For farmers and growers, the goal is to provide on-site microbial inspection results so that they may take measures internally without going through reporting and full-scale inspections. This effort may help each of the stakeholders so that they may take the initiative of ensuring effective microbial inspection and keep the food supply safe for all.This I-Corps project is based on the development of a portable food safety instrument. For the food safety area, the highlight is the bacteriophage-based assay that has high specificity with respect to the target food-borne pathogen, E.coli O157:H7. This patented assay only infects the target bacteria by integrating into the chromosome and does not kill the bacteria itself. At the same time, integrated genes are coded to generate photons at a wavelength around 495 nm. While conventional methods require a laboratory-based, high sensitivity measurement system (a luminometer) to detect the low level photon signal, this technology is pushing the limits of conventional, complementary metal oxide semiconductor (CMOS)-based senors, which are often used in smartphone cameras. The proposed hardware design for efficient photon capturing and the ensemble averaging-based noise reduction algorithm enables the detection of extremely low levels of light from commercial smartphones. Food spike testing with ground beef as a food matrix have resulted in positive signal detection within 10-12 hrs after inoculation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

I-Corps项目更广泛的影响/商业潜力是开发一个可现场部署的食品安全评估平台。目前的规程要求将食品样品运送到中央实验室进行进一步分析。接受检测的食品将已经出售并被消费者消费。这项工作的目的是在10-12小时内提供检查结果，以便制造商及时反馈，并使数据库选项能够数字化管理检查结果和记录，以便这些信息可以潜在地集成到即将到来的食品安全领域的区块链革命中。成功将为食品病原体的检测提供更早的时间，以便监管机构能够对潜在的疫情情况实施更快速的对策。对于制造商来说，目标是及时提供检测结果，以便制造商自己进行检查，找到易受污染的地方。对于农民和种植者来说，目标是提供现场微生物检查结果，以便他们可以在内部采取措施，而不必经过报告和全面检查。这项工作可以帮助每个利益相关者，使他们能够主动确保有效的微生物检查，并保证所有人的食品供应安全。I-Corps项目的基础是开发一种便携式食品安全仪器。在食品安全领域，重点是基于噬菌体的检测，它对目标食源性病原体大肠杆菌O157:H7具有高特异性。这项专利试验仅通过整合到染色体中来感染目标细菌，而不会杀死细菌本身。与此同时，整合的基因被编码为产生波长在495nm左右的光子。传统的方法需要基于实验室的高灵敏度测量系统（一个光度计）来检测低能级光子信号，而这项技术正在突破传统的基于互补金属氧化物半导体（CMOS）的传感器的极限，这种传感器通常用于智能手机相机。所提出的高效光子捕获硬件设计和基于集成平均的降噪算法能够检测来自商用智能手机的极低水平的光。在接种后10-12小时内，以碎牛肉作为食物基质进行的食物刺突试验产生了阳性信号检测。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

A smartphone-based bacterial colony phenotyping instrument based on the reflective elastic light-scatter pattern

基于智能手机的基于反射弹性光散射图案的细菌菌落表型分析仪

• DOI: 10.1117/12.2623007
• 发表时间: 2022
• 期刊: Sensing for Agriculture and Food Quality and Safety XIV
• 作者: Doh, Iyll-Joon;Dowden, Brianna;Patsekin, Valery;Rajwa, Bartek;Robinson, J. Paul;Bae, Euiwon
• 通讯作者: Bae, Euiwon