Collaborative Research: RUI: Implications of bacterially driven cross-kingdom chemical interactions

合作研究：RUI：细菌驱动的跨界化学相互作用的影响

• 批准号: 2041435
• 负责人: Julia Kubanek
• 金额: $ 24.01万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2025-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2041435&HistoricalAwards=false
• 关键词: Collaborative; Research; RUI; Implications; bacterially

Over evolutionary history, microbes have evolved in close association with larger, more complex organisms, often driven by the mutually beneficial exchange of nutrients. Advancements in analytical techniques have revealed that many host-bacterial interactions are tightly regulated by small molecules, acting as chemical messengers. Yet, connecting how these interactions at the cellular level translate to impacts on whole communities and ecosystems remains an ongoing challenge necessitating cross-disciplinary collaboration. A newly emerging picture suggests that marine bacteria are not passive in their interactions with hosts such as algae; rather bacteria actively mediate the flow of organic matter depending on how they deploy their chemical arsenal. The assembled interdisciplinary team will develop new methods to track and translate complex chemical signals between a model algal host and associated bacteria. New methodologies in fluorescent biosensors will quantify bacterial production of chemical signals at the cellular level, whose release manipulates host physiology. How the host responds to the chemicals will be assessed by measuring changes in the host's proteins, RNA, and metabolites. This work supports the training and mentoring of underrepresented/underresourced postdoctoral and undergraduate trainees in themes bridging the chemical, biological, and environmental sciences. Four postdoctoral investigators will participate in course-based undergraduate research experiences with Haverford faculty with the goal of building supportive scholar-teacher mentoring networks with students and providing training in equitable and inclusive course design so that the scholars becoming more reflective, engaged, and effective teachers. All four PIs will develop conference-based experiential workshops to provide career development.Microbes have emerged as key players in marine systems where they form complex relationships with eukaryotic phytoplankton. These interactions range from symbiotic to pathogenic and are tightly regulated through the dynamic exchange of small molecule chemical messengers that shape communities and influence the biogeochemical fate of oceanic nutrients. Efforts to elucidate the exchange of chemical signals and decipher the environmental drivers of bacterial-phytoplankton interactions are limited and met with substantial technical challenges. Recently, two chemical messengers, tetrabromopyrrole (TBP) and 2-heptyl-4-quinolone (HHQ), both produced by the globally distributed marine gamma proteobacterium, Pseudoalteromonas, have been identified to govern the physiology of its associated, bloom-forming single-cell algal host, Emiliania huxleyi, with contrasting modalities. Experiments outlined in this study are poised to tease apart the metabolic landscape defining this model host-bacterial symbiosis, and detail when and how chemical messengers induce host metabolic reprogramming resulting in ecosystem level consequences. Three synergistic aims will be accomplished: in the first, TBP and HHQ production and uptake will be measured in response to nutrient variability and HHQ production will be captured at the single-cell level using biosensors. In the second aim, experiments will reveal metabolic cross-talk by establishing the metabolomic and proteomic response of E. huxleyi to both P. piscicida and HHQ, while tracking P. piscicida uptake of E. huxleyi-derived metabolites. In the third, experiments will investigate if HHQ prevents E. huxleyi viral entry using electron microscopy, accounting for an earlier observation that viral-induced mortality of E. huxleyi is stalled in the presence of HHQ.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在进化史上，微生物与更大，更复杂的生物体密切相关，通常由互惠的营养交换驱动。分析技术的进步表明，许多宿主与细菌的相互作用受到小分子的严格调控，这些小分子充当化学信使。然而，如何将这些在细胞水平上的相互作用转化为对整个社区和生态系统的影响仍然是一个持续的挑战，需要跨学科的合作。一个新出现的图片表明，海洋细菌在与藻类等宿主的相互作用中并不是被动的;相反，细菌根据它们如何部署化学武器库来主动介导有机物质的流动。组装的跨学科团队将开发新的方法来跟踪和翻译模型藻类宿主和相关细菌之间的复杂化学信号。荧光生物传感器的新方法将在细胞水平上量化细菌产生的化学信号，这些信号的释放操纵宿主的生理学。宿主对化学物质的反应将通过测量宿主蛋白质、RNA和代谢物的变化来评估。这项工作支持培训和指导代表性不足/资源不足的博士后和本科生学员的主题桥接化学，生物和环境科学。四名博士后研究人员将与哈弗福德教师一起参加基于课程的本科研究经验，目标是与学生建立支持性的教师指导网络，并提供公平和包容性课程设计的培训，使学者成为更具反思性，参与性和有效的教师。所有四个PI将开发基于会议的体验式研讨会，以提供职业发展。微生物已成为海洋系统中的关键角色，它们与真核浮游植物形成复杂的关系。这些相互作用的范围从共生到致病，并通过小分子化学信使的动态交换进行严格调控，这些信使塑造了群落并影响了海洋营养物质的生物地球化学命运。阐明化学信号交换和破译细菌-浮游植物相互作用的环境驱动因素的努力是有限的，并遇到了重大的技术挑战。最近，两个化学信使，四溴吡咯（TBP）和2-庚基-4-喹诺酮（HHQ），都产生了全球分布的海洋伽马变形杆菌，假交替单胞菌，已被确定为管理其相关的，形成水华的单细胞藻类宿主，埃米利亚huxleyi的生理，与对比的方式。本研究中概述的实验准备梳理定义这种模式宿主-细菌共生的代谢景观，并详细说明化学信使何时以及如何诱导宿主代谢重编程，从而导致生态系统水平的后果。将实现三个协同目标：第一，TBP和HHQ的生产和吸收将被测量，以响应营养的变化和HHQ生产将被捕获在单细胞水平上使用生物传感器。在第二个目标中，实验将揭示代谢串扰通过建立代谢组学和蛋白质组学反应的E。huxleyi对杀鱼原蝇和HHQ的吸收，同时跟踪杀鱼原蝇对E.赫胥黎衍生代谢物。第三部分是实验研究HHQ是否能阻止E. huxleyi病毒进入，解释了早期观察到的病毒诱导的E.这个奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

Quorum sensing signal disrupts viral infection dynamics in the coccolithophore Emiliania huxleyi

群体感应信号破坏了颗石藻赫胥黎的病毒感染动态

• DOI: 10.3354/ame01998
• 发表时间: 2023
• 期刊: Aquatic Microbial Ecology
• 影响因子: 1.4
• 作者: Harvey, EL;Yang, H;Castiblanco, E;Coolahan, M;Dallmeyer-Drennen, G;Fukuda, N;Greene, E;Gonsalves, M;Smith, S;Whalen, KE
• 通讯作者: Whalen, KE

Probing the Phycosphere: Techniques to Study Bacteria-Phytoplankton Interactions

• DOI: 10.1093/icb/icad065
• 发表时间: 2023-06-09
• 期刊: INTEGRATIVE AND COMPARATIVE BIOLOGY
• 影响因子: 2.6
• 作者: Platt，Amanda J.;Whalen，Kristen E.
• 通讯作者: Whalen，Kristen E.