CAREER: Paralog function following rapid gene family expansion in Candida albicans

职业：白色念珠菌基因家族快速扩张后的旁系同源功能

• 批准号: 2046863
• 负责人: Matthew Anderson
• 金额: $ 144.33万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2046863&HistoricalAwards=false
• 关键词: CAREER; Paralog; function; following; rapid

This project seeks to determine how multiple genes that all arose recently from a single gene establish their function when other copies of that gene are present. Nearly all genes in every organism on the planet arose from previously existing genes and not from DNA that lacked function. As this process repeats, a large set of highly similar genes can form a gene family that often give an organism certain advantages in adapting to its specific lifestyle. For example, yeasts used in brewing have expanded gene families for fermentation and humans have expanded genes for being able to distinguish between good and rotten food. A gene family in the human fungal pathogen Candida albicans called the telomere-associated (TLO) gene family has expanded from 1 to 14 copies and is an ideal system to test how functions of individual members of gene families are shaped by the presence of many genetically and functionally similar genes. This project will generate large datasets and highlights the needs for people to be trained in analysis. This project will therefore also train Indigenous students in bioinformatics through a summer workshop to promote data sovereignty and Indigenous research capacity. This project will begin to address the concept of Ohno’s Dilemma in a large gene family, how function evolves when multiple other similar genes are present. We will use the TLO genes from C. albicans to find how each gene holds individual or overlapping functions with other genes in the gene family. Molecular and organismal functions will be determined for these genes using strains missing single genes of the gene family and strains lacking all TLO genes except for one. Strains lacking single genes will define the amount of overlap in gene functions, and strains containing single genes will determine the range of function for each gene.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目旨在确定当存在该基因的其他副本时，全部来自单个基因的多个基因如何建立其功能。地球上每个有机化机中的几乎所有基因都源于先前现有的基因，而不是源于缺乏功能的DNA。随着这个过程的重复，一系列高度相似的基因可以形成一个基因家族，该基因家族通常会在适应其特定生活方式方面具有某些优势。例如，用于酿造中的酵母菌已经扩大了发酵的基因家族，人类扩大了基因，因为它能够区分好食物和腐烂的食物。人类真菌病原体念珠菌中的一个基因家族称为端粒相关（TLO）基因家族已从1份扩展到14份，并且是一个理想的系统，可以测试基因家族成员的功能如何通过许多遗传和功能相似的基因来塑造基因家族的功能。该项目将生成大型数据集，并突出人们在分析中接受培训的需求。因此，该项目还将通过夏季研讨会来培训土著学生的生物信息学学生，以促进数据主权和土著研究能力。该项目将开始解决大型基因家族中Ohno困境的概念，当存在多个其他类似基因时功能如何发展。我们将使用白色念珠菌的TLO基因来找到每个基因如何与基因家族中其他基因的个体或重叠函数保持。将使用缺少基因家族的单基因的菌株来确定这些基因的分子和有机功能，除一个基因所缺乏的菌株以外的所有TLO基因。缺乏单个基因的菌株将定义基因函数的重叠量，而包含单个基因的菌株将确定每个基因的功能范围。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响标准来评估通过评估来诚实地表示支持。

项目成果

A relational framework for microbiome research with Indigenous communities

• DOI: 10.1038/s41564-023-01471-2
• 发表时间: 2023-10-01
• 期刊: NATURE MICROBIOLOGY
• 影响因子: 28.3
• 作者: Bader，Alyssa C.;Van Zuylen，Essie M.;Anderson，Matthew Z.
• 通讯作者: Anderson，Matthew Z.

Microbiome ownership for Indigenous peoples

• DOI: 10.1038/s41564-023-01470-3
• 发表时间: 2023-09
• 期刊: Nature Microbiology
• 影响因子: 28.3
• 作者: M. Handsley-Davis;Matthew Z. Anderson;Alyssa C. Bader;Hanareia Ehau-Taumaunu;Keolu Fox;Emma Kowal;L. Weyrich

Balancing openness with Indigenous data sovereignty: An opportunity to leave no one behind in the journey to sequence all of life.

• DOI: 10.1073/pnas.2115860119
• 发表时间: 2022-01-25
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Mc Cartney AM;Anderson J;Liggins L;Hudson ML;Anderson MZ;TeAika B;Geary J;Cook-Deegan R;Patel HR;Phillippy AM
• 通讯作者: Phillippy AM