Molecular Control of Vascular Meristem Initiation and Activity

血管分生组织起始和活性的分子控制

• 批准号: 2049926
• 负责人: Huanzhong Wang
• 金额: $ 55.49万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2049926&HistoricalAwards=false
• 关键词: Molecular; Control; Vascular; Meristem; Initiation

The plant vascular system plays essential roles in transporting water and nutrients to all growing organs. In addition, woody fibers of the vascular system provide mechanical support for plant upright growth. Xylary wood also forms the majority of plant biomass on earth and serves as feedstock for biofuel production. Therefore, the vascular system is important to plant growth and development and woody biomass production. Despite the importance of the vascular system, it is challenging to study vascular initiation because the vascular tissue is buried under layers of other cell types and inaccessible for direct microscopic observation. The vascular system is initiated from a region named the rib zone that is located below the plant growing tip. This research investigates how plants initiate vascular bundles and identifies genes that regulate vascular cell division using recently discovered genes and genetic methodologies. Outcomes of this research include a better understanding of vascular tissue formation and the regulation of cell proliferation in vascular tissues. This project integrates research and education by engaging under-represented undergraduate and high school students.The vascular system transports water and nutrients and has allowed plant species to successfully colonize various of terrestrial environments since they evolved 450 Million years ago. The vascular system initiates from the apical meristem rib zone and organizes as vascular bundles. Cambial cells from vascular bundles proliferate and then differentiate into phloem and xylem. Understanding the regulation of vascular meristem initiation and activity is of great interest to plant biology research. Further, xylary wood serves as a renewable feedstock for biofuel production. Despite the importance of vascular tissue, there is a knowledge gap in our understanding of the initiation and activity control of vascular meristem. This project builds on the identification of a novel C2H2 transcription factor High Vascular Activity (HVA) to dissect the regulatory mechanisms of vascular initiation and meristematic cell proliferation. The first aim is to investigate the molecular function of HVA in regulating vascular initiation using deep imaging approaches. The second aim is to elucidate the functional mechanism of HVA in modulating vascular meristem activity. The third aim is to determine the function of HVA in xylem cell differentiation using genetic, biochemical, and transgenic studies. Overall, this project will lay the ground for molecular dissection of vascular meristem initiation and cambial proliferation in the stem.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物维管系统在将水分和营养物质输送到所有生长器官中起着至关重要的作用。此外，维管系统的木质纤维为植物直立生长提供机械支撑。木质木材也构成了地球上大部分的植物生物质，并作为生物燃料生产的原料。因此，维管系统对植物的生长发育和木本生物量的生产具有重要意义。尽管血管系统的重要性，它是具有挑战性的研究血管的起始，因为血管组织被埋在其他细胞类型的层和直接显微镜观察无法访问。维管系统起源于位于植物生长顶端下方的肋区。本研究利用新近发现的基因和遗传学方法研究了植物如何启动维管束，并鉴定了调节维管细胞分裂的基因。这项研究的结果包括更好地了解血管组织的形成和血管组织中细胞增殖的调节。该项目通过吸引代表性不足的本科生和高中生来整合研究和教育。维管系统运输水和营养物质，并允许植物物种自4.5亿年前进化以来成功地在各种陆地环境中定居。维管系统起源于顶端分生组织肋区，组织成维管束。维管束形成层细胞增殖后分化为韧皮部和木质部。了解维管分生组织发生和活动的调控是植物生物学研究的重要内容。此外，木质木材用作生物燃料生产的可再生原料。尽管维管组织的重要性，有一个知识的差距，我们的理解的启动和活性控制的维管分生组织。本研究以一个新的C2H2转录因子High Vascular Activity（HVA）的发现为基础，探讨其在维管起始和分生组织细胞增殖中的调控机制。第一个目的是研究分子功能的HVA在调节血管启动使用深成像方法。第二个目的是阐明HVA调节维管分生组织活动的功能机制。第三个目的是通过遗传、生物化学和转基因研究确定HVA在木质部细胞分化中的功能。总的来说，这个项目将为血管分生组织起始和茎中形成层增殖的分子解剖奠定基础。这个奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为是值得支持的。