In vivo tumor imaging with para-hydrogen

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The main goal of the proposed project is the development of an inexpensive alternative to investigate molecular processes in tumors, which shows great potential for clinical applications because of its cost efficiency and its easiness to handle. This goal will be achieved by combining easy processes of hyperpolarizing amino acid derivatives with magnetic resonance tomography (MRT).In the course of the project magnetic resonance studies of amino acids and small peptides (NGR, cRGD), which will be hyperpolarized by Para-Hydrogen Induced Polarization (PHIP) and especially with the Signal Amplification by Reversible Exchange-method (SABRE), will be undertaken. The second part of the project will focus on in-vitro studies using flow cells. Carcinoma cells will be overflowed with hyperpolarized tracers and signal-time-curves will be recorded with dynamic MRT measurements and evaluated pharmacokinetically. The third part of the project contains in-vivo experiments with tumor-bearing rats to investigate the potential to analyze molecular processes in tumors with MRT.