Molecular and Evolutionary Mechanisms Underlying Desiccation Resistance Across Drosophila Species

果蝇物种抗干燥性的分子和进化机制

• 批准号: 2054773
• 负责人: Henry Chung
• 金额: $ 78.65万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2054773&HistoricalAwards=false
• 关键词: Molecular; Evolutionary; Mechanisms; Underlying; Desiccation

Understanding how current species adapt to extreme environments such as the desert can help determine how the species could evolve in response to environmental changes. As environments get warmer and drier due to rapid climate change, organisms must adapt to these changes to survive and thrive in these new conditions. Insects use a lipid layer on their body surface to prevent water loss and withstand desiccation stress. The proposed study will investigate the association between desiccation resistance and chemical properties of this lipid layer across insect species. Furthermore, this study will use a desert-dwelling insect species as a model to investigate the physiological and genetic mechanisms underlying how insects can evolve very high desiccation resistance. Since components of this lipid layer also function as pheromones in many insect species, evolutionary changes in these components can lead to changes in mating successes. Results from this study will provide empirical evidence in determining how environmental adaptation can lead to changes in mating behaviors and reproductive isolation between insect species. The project will use existing infrastructure to promote STEM teaching, training, and learning to students, teachers, and the public through workshops, videos, and science fair participation, as well as broaden full participation of underrepresented minorities.Adaptation to different environments and habitats is key to long-term species persistence. Decreasing water loss is important for organisms adapting to different terrestrial environments. In Drosophila fruit flies and other terrestrial insects, this ability to prevent water loss is crucial. Previous studies suggested that cuticular water loss accounts for the majority of water loss in insects and hypothesized that differences in cuticular hydrocarbon (CHC) content account for differences in desiccation resistance between mesic and desert species. However, the specific association between different CHC components and desiccation has not been established, and the genetic mechanisms underlying the evolution of these CHC components that confer high desiccation resistance have not been elucidated. This proposed study will investigate how evolution of CHCs in insects affects desiccation resistance and elucidates the genetic mechanisms and evolutionary constraints that govern their evolution in a desert species, Drosophila mojavensis. As CHCs are dual traits that also have other functions such as mate recognition, the constraints that govern the evolution of these CHCs in these dual roles are not well understood. This project will also investigate how the functions of CHCs in desiccation resistance may affect mate recognition. Collectively, the long-term goal of this project is to elucidate the molecular and evolutionary mechanisms that enable species to withstand water loss and maintain water balance as our planet gets warmer and more arid in the next few decades.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

了解当前物种如何适应沙漠等极端环境，有助于确定该物种如何随着环境变化而进化。由于快速的气候变化，环境变得越来越温暖和干燥，生物必须适应这些变化，才能在这些新的条件下生存和茁壮成长。昆虫在它们的身体表面使用一层脂层来防止水分流失和抵抗干燥压力。这项拟议的研究将调查不同种类昆虫的抗干性和这种脂层的化学性质之间的联系。此外，这项研究将以一种生活在沙漠中的昆虫物种为模型，研究昆虫如何进化出非常高的脱水抗性的生理和遗传机制。由于这种脂层的成分在许多昆虫物种中也起信息素的作用，这些成分的进化变化可能会导致交配成功的变化。这项研究的结果将为确定环境适应如何导致昆虫物种之间的交配行为和生殖隔离的变化提供经验证据。该项目将利用现有的基础设施，通过研讨会、视频和科学博览会的参与，向学生、教师和公众推广STEM的教学、培训和学习，并扩大代表不足的少数民族的充分参与。适应不同的环境和栖息地是物种长期生存的关键。减少水分损失对生物适应不同的陆地环境很重要。在果蝇和其他陆地昆虫中，这种防止水分流失的能力是至关重要的。以往的研究表明，表皮层失水是昆虫失水的主要原因，并假设表皮层碳氢化合物(CHC)含量的差异是中生物种和荒漠物种脱水抗性差异的原因。然而，不同的CHC组分与脱水之间的具体联系尚未建立，这些CHC组分进化的遗传机制尚未阐明。这项拟议的研究将调查昆虫中CHCs的进化如何影响脱水阻力，并阐明控制它们在沙漠物种--果蝇中进化的遗传机制和进化制约因素。由于CHC是双重特征，还具有其他功能，如识别配偶，控制这些CHC在这些双重角色中进化的制约因素还没有被很好地理解。该项目还将调查CHC在脱水抵抗中的作用如何影响配偶识别。总而言之，该项目的长期目标是阐明分子和进化机制，使物种能够承受水分损失，并在未来几十年地球变暖和干旱时保持水平衡。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Natural variation at a single gene generates sexual antagonism across fitness components in Drosophila

• DOI: 10.1016/j.cub.2022.05.038
• 发表时间: 2022-06
• 期刊: Current Biology
• 影响因子: 9.2
• 作者: B. Rusuwa;Henry Chung;S. Allen;F. Frentiu;S. Chenoweth