Identification and characterization of cell wall integrity monitoring components in plants

植物细胞壁完整性监测成分的鉴定和表征

基本信息

  • 批准号:
    246277104
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    德国
  • 项目类别:
    Research Fellowships
  • 财政年份:
    2013
  • 资助国家:
    德国
  • 起止时间:
    2012-12-31 至 2015-12-31
  • 项目状态:
    已结题

项目摘要

Functional integrity of plant cell walls has to be maintained enabling them to perform their functions during development and defence against biotic and abiotic stress. This functional integrity is influenced by cell wall composition, structure and cellular metabolism. Recent studies have shown that cell wall damage (CWD) impairing functional integrity is being detected in plants and specific compensatory reactions are initiated to maintain integrity. These reactions involve changes in cell wall composition and structure, in production of phytohormones and in carbohydrate metabolism. In particular the effects on carbohydrate metabolism are sensitive to manipulation by osmotica. The host lab has established an Arabidopsis seedling-based model system to study the mode of action of the CWD response mechanism. In the context of this fellowship I aim to execute two subprojects. First, I will characterize gene functions necessary for CWD-induced compensatory reactions. Based on transcriptional and preliminary phenotypic data collected in the host lab, several candidate genes have already been implicated in cell wall metabolism and the response to CWD. I will confirm these preliminary results and extend them specifically with respect to detailed cell wall analyses and the effect on resistance towards cell wall penetrating fungal pathogens in gene knockouts to determine biological functions of candidate genes. The second subproject will focus on the identification and characterization of early signalling components of the CWD response. It has already been shown that CWD induces post-translational protein modification. Furthermore, the putative stretch-activated Ca2+ channel MCA1 is required for the osmosensitive components of the CWD response. Therefore I will analyse the impact of CWD and osmotic support on the phosphoproteome of wild type and mca1 seedlings in order to identify proteins that are post-translationally modified in response to CWD in an osmosensitive way. I will then use knockout lines for the candidate genes to investigate their function in CWD response and particularly in carbohydrate partitioning. The aims of this project are locating it at the intersection between plant physiology, cell biology and phytopathology and therefore, novel insights regarding the cell wall integrity maintenance mechanism will be relevant for all three areas. New insights in turgor-dependent regulatory processes will also strengthen the link between biophysical and biochemical research.
必须保持植物细胞壁的功能完整性,使它们能够在发育和防御生物和非生物胁迫期间发挥其功能。这种功能完整性受细胞壁组成、结构和细胞代谢的影响。最近的研究表明,细胞壁损伤(CWD)损害功能的完整性正在被检测到的植物和特定的补偿反应启动,以保持完整性。这些反应涉及细胞壁组成和结构的变化,植物激素的产生和碳水化合物的代谢。特别是对碳水化合物代谢的影响对植物的操纵敏感。本实验室建立了一个以拟南芥幼苗为基础的模型系统,研究CWD反应机制的作用方式。在这个奖学金的背景下,我的目标是执行两个子项目。首先,我将描述CWD诱导的代偿反应所必需的基因功能。基于宿主实验室收集的转录和初步表型数据,几个候选基因已经涉及细胞壁代谢和对CWD的反应。我将确认这些初步结果,并将其具体扩展到详细的细胞壁分析和基因敲除对穿透细胞壁的真菌病原体的抗性的影响,以确定候选基因的生物学功能。第二个分项目将侧重于识别和描述慢性消耗病反应的早期信号成分。已经表明CWD诱导翻译后蛋白质修饰。此外,推定的牵张激活的Ca 2+通道MCA 1所需的CWD响应的神经元敏感组件。因此,我将分析的影响,CWD和渗透支持的磷酸化蛋白质组的野生型和mca 1幼苗,以确定蛋白质的后处理修改响应CWD在一个敏感的方式。然后,我将使用候选基因的敲除系来研究它们在CWD反应中的功能,特别是在碳水化合物分配中的功能。该项目的目的是将其定位于植物生理学,细胞生物学和植物病理学之间的交叉点,因此,关于细胞壁完整性维持机制的新见解将与所有三个领域相关。对turgor-dependent调节过程的新认识也将加强生物物理和生物化学研究之间的联系。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Dr. Timo Engelsdorf其他文献

Dr. Timo Engelsdorf的其他文献

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{{ truncateString('Dr. Timo Engelsdorf', 18)}}的其他基金

Regulation of pathogen defence by plant cell wall integrity signalling
通过植物细胞壁完整性信号调节病原体防御
  • 批准号:
    471591813
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Research Grants

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