Folding Colloidomers into Functional 3D Architectures

将胶体折叠成功能性 3D 架构

• 批准号: 2105255
• 负责人: Jasna Brujic
• 金额: $ 55万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2025-06-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2105255&HistoricalAwards=false
• 关键词: Folding; Colloidomers; into; Functional; 3D

NON-TECHNICAL SUMMARY:This proposal aims to build complex materials through the art of folding. Just like chains of amino acids fold into proteins to perform a specific biological function, here short chains of droplets coated with DNA are designed to fold into preprogrammed novel materials. These folded structures serve as building blocks to seed further assembly into unusual crystals with symmetries that are as yet unexplored. It has been predicted that the creation of crystalline structures on the same length scale as the wavelength of light can lead to materials with unique properties, such as structural color. This work could lead to the development of new dyes or even the creation of an optical transistor. A longstanding goal is to integrate science education and research activities within the laboratory and beyond. The PI is a founding member of a science education facility called the Biobus, which is a mobile lab that provides school students from all socioeconomic backgrounds with a research grade microscopy facility and welcomes 20,000 K-12 students every year.TECHNICAL SUMMARY:The past decade has witnessed more major innovations in colloidal materials than in the entire previous history of the field. Instead of classical colloids with interactions such as Van der Waals and depletion attraction that lead to aggregates or colloidal crystals, modern designer particles come with controllable chemistry, specific interactions enabled by functionalization with complementary DNA strands, directed by patches and their geometry. Here the goal is to modify self-assembly via folding and induce programmable arbitrary structures. Emulsion droplets serve as slippery designer particles, which can reorganize during self-assembly. Inspired by Nature’s creation of 3D materials via folding mechanisms, linear colloidomer chains of droplets will fold by secondary DNA interactions into programmable 2D or 3D architectures to make functional materials. Emulsion folding constitutes a nonequilibrium system, driven by temperature protocols to reversibly unfold and refold. The experiments are guided by numerical simulations and theory, which optimizes the interaction matrices, as well as the time-dependent drive, in order to achieve high yield and fidelity of structures with arbitrary shapes. The goal is to achieve well-defined functionalities, while revealing self-organizing non-equilibrium principles underlying droplet folding. As part of the broader impacts, this project will yield 'Mayonnaise Robots' curriculum module for educational outreach to K-12 students, which will allow students to design and build their own folds and familiarize themselves with the art and science of programmable design.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

非技术概述：本提案旨在通过折叠艺术构建复杂的材料。就像氨基酸链折叠成蛋白质来执行特定的生物功能一样，这里包裹着DNA的短链液滴被设计成折叠成预先编程的新材料。这些折叠的结构可以作为积木，为进一步组装成不寻常的晶体提供种子，这些晶体具有尚未被探索的对称性。据预测，在与光的波长相同的长度尺度上创造晶体结构可以导致具有独特性质的材料，例如结构颜色。这项工作可能会导致新染料的发展，甚至是光学晶体管的创造。一个长期的目标是在实验室内外整合科学教育和研究活动。PI是一个名为Biobus的科学教育设施的创始成员，这是一个移动实验室，为来自所有社会经济背景的学生提供研究级显微镜设备，每年欢迎20,000名K-12学生。技术总结：在过去的十年中，胶体材料的重大创新比该领域以前的全部历史都要多。经典的胶体具有范德华效应和耗竭吸引等相互作用，导致聚集体或胶体晶体，而现代的设计粒子具有可控的化学性质，通过与互补DNA链的功能化实现特定的相互作用，由斑块和它们的几何形状指导。这里的目标是通过折叠和诱导可编程的任意结构来修改自组装。乳状液滴作为光滑的设计颗粒，可以在自组装过程中重新组织。受大自然通过折叠机制创造3D材料的启发，液滴的线性胶体链将通过次级DNA相互作用折叠成可编程的2D或3D结构，以制造功能材料。乳剂折叠构成了一个非平衡系统，在温度协议的驱动下可逆地展开和折叠。实验以数值模拟和理论为指导，优化相互作用矩阵和时间相关驱动，以实现任意形状结构的高良率和保真度。目标是实现定义良好的功能，同时揭示液滴折叠背后的自组织非平衡原理。作为更广泛影响的一部分，该项目将为K-12学生提供教育推广的“蛋黄酱机器人”课程模块，这将允许学生设计和构建自己的折叠，并熟悉可编程设计的艺术和科学。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Push-pull mechanics of E-cadherin ectodomains

E-钙粘蛋白胞外域的推拉机制

• 发表时间: 2023
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: K. Nagendra, A. Izzet

Self-assembly of emulsion droplets through programmable folding

• DOI: 10.1038/s41586-022-05198-8
• 发表时间: 2022-09-28
• 期刊: NATURE
• 影响因子: 64.8
• 作者: McMullen, Angus;Basagoiti, Maitane Munoz;Brujic, Jasna
• 通讯作者: Brujic, Jasna