Antimicrobial-Membrane Interactions in Living Bacteria
活细菌中抗菌剂与膜的相互作用
基本信息
- 批准号:2105387
- 负责人:
- 金额:$ 51.07万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
With the support of the Chemistry of Life Processes (CLP) Program in the Division of Chemistry, Professor Hai-Lung Dai of Temple University is studying how bacterial membranes regulate their interactions with the surrounding environment, in particular those containing antimicrobial agents. The goal of this project is to provide fundamental knowledge that holds potential for future development of more effective antimicrobials that destroy disease-causing bacteria and reduce bacterial resistance to antibiotics. The role of small molecules and synthetic proteins in changing the permeability of bacterial cell membranes will be studied by quantitatively examining the response of small molecules whose interaction with light allows for their very sensitive detection when bound to cell membrane components. This powerful light-based method will be used to test how tiny holes are formed in the membrane upon membrane interaction with nearby chemicals or by changing how molecules move through channels in the membrane of bacteria. Professor Dai will integrate high school, college, and PhD students into the research program, providing a highly diverse group of students a very broad scientific skill set to help them pursue their career goals. Further broadening the impact of the project is a summer camp for Philadelphia inner city middle school students. In sum, the project will help strengthen the scientific workforce by training and exposing young minds to research aimed at providing answers to important fundamental questions about the chemistry of bacterial membranes with the ultimate goal of providing information key to addressing bacterial infections and antibiotic resistance. The significance of the research area is evidenced by the growing problem of antimicrobial-resistant bacterial infections, which are predicted by the World Health Organization and the United Nations to result in the death of 10 million people annually by 2050. In this study, Professor Dai will examine how the complex wall structures of bacteria exercise their regulatory functions on external molecules like antimicrobials. We will delineate, using both nonlinear optical spectroscopy and microscopy approaches, a variety of membrane transport mechanisms. These will include those based on import channels, efflux pumps, and lipid bilayer permeability, which play key roles in regulating intracellular concentrations of molecules critical to bacteria viability and protecting the bacteria from harmful compounds or an over-abundance of what would otherwise be thought to be useful compounds. The research will provide fundamental understanding of how antimicrobials interact with the various components of bacterial membranes to make them more permeable and reduce the protective functions of the membrane. Experiments will be designed to gather data critical for understanding how membranes perform their regulatory functions for living cells, as well as for designing strategies for developing more effective antimicrobials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在天普大学化学系生命过程化学(CLP)项目的支持下,戴海龙教授正在研究细菌膜如何调节其与周围环境的相互作用,特别是那些含有抗菌药物的细菌膜。该项目的目标是提供基础知识,为未来开发更有效的抗菌素提供潜力,这些抗菌素可摧毁致病细菌并减少细菌对抗生素的耐药性。小分子和合成蛋白在改变细菌细胞膜渗透性中的作用将通过定量检查小分子的反应来研究,这些小分子与光的相互作用允许它们在与细胞膜成分结合时非常敏感地检测到。这种强大的基于光的方法将被用来测试当膜与附近的化学物质相互作用时,膜上是如何形成小孔的,或者通过改变分子在细菌膜上的通道中移动的方式。戴教授将把高中生、大学生和博士生纳入研究项目,为高度多样化的学生提供广泛的科学技能,帮助他们追求自己的职业目标。进一步扩大项目影响的是费城内城中学生的夏令营。总而言之,该项目将通过培训和让年轻人参与研究,帮助加强科学队伍,旨在为细菌膜化学的重要基本问题提供答案,最终目标是提供解决细菌感染和抗生素耐药性的关键信息。这一研究领域的重要性得到了日益严重的抗微生物药物耐药性细菌感染问题的证明,据世界卫生组织和联合国预测,到2050年,每年将有1 000万人死亡。在这项研究中,戴教授将研究细菌复杂的壁结构如何对抗菌剂等外部分子行使其调节功能。我们将描述,使用非线性光学光谱和显微镜方法,各种膜运输机制。这些将包括那些基于进口通道、外排泵和脂质双分子层渗透性的机制,它们在调节细胞内分子浓度方面发挥关键作用,这些分子对细菌的生存至关重要,并保护细菌免受有害化合物或过量的有害化合物的侵害,否则被认为是有用的化合物。该研究将提供基本的了解抗菌剂如何与细菌膜的各种成分相互作用,使其更具渗透性并降低膜的保护功能。实验的目的是收集关键数据,以了解膜如何发挥其对活细胞的调节功能,以及为开发更有效的抗菌剂设计策略。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Hai-Lung Dai其他文献
Ultraviolet laser-induced formation of thin silicon oxide film from the precursor β-chloroethyl silsesquioxane
- DOI:
10.1557/jmr.1999.0132 - 发表时间:
2011-01-31 - 期刊:
- 影响因子:2.900
- 作者:
Jaya Sharma;Donald H. Berry;Russell J. Composto;Hai-Lung Dai - 通讯作者:
Hai-Lung Dai
Time-Resolved Studies of Adsorption and Transport of a Hydrophobic Ion at Escherichia coli Bacterial Membranes by Second Harmonic Generation
- DOI:
10.1016/j.bpj.2008.12.654 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Jia Zeng;Heather Eckenrode;Hai-Lung Dai - 通讯作者:
Hai-Lung Dai
The effect of adsorption of Na on Cu(1 1 0) surface states probed by second harmonic generation
- DOI:
10.1016/j.susc.2005.03.047 - 发表时间:
2005-06-01 - 期刊:
- 影响因子:
- 作者:
Susan M. Dounce;Hai-Lung Dai - 通讯作者:
Hai-Lung Dai
Aniline on Ag(1 1 1): Adsorption configuration, adsorbate–substrate bond, and inter-adsorbate interactions
- DOI:
10.1016/j.susc.2005.05.048 - 发表时间:
2005-09-01 - 期刊:
- 影响因子:
- 作者:
Thomas J. Rockey;Minchul Yang;Hai-Lung Dai - 通讯作者:
Hai-Lung Dai
Carrier recombination of organic-inorganic 3D halide perovskite single crystals
有机-无机3D卤化物钙钛矿单晶的载流子复合
- DOI:
10.1063/1674-0068/cjcp1911197 - 发表时间:
2020-05 - 期刊:
- 影响因子:0
- 作者:
Chao He;Xia Li;Yu-hao Wu;Hai-Lung Dai;Dong-feng Zhao;Yang Chen - 通讯作者:
Yang Chen
Hai-Lung Dai的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Hai-Lung Dai', 18)}}的其他基金
Nonlinear Light Scattering Spectroscopy and Microscopy of Molecular Interactions at Biological Surfaces
生物表面分子相互作用的非线性光散射光谱和显微镜
- 批准号:
1465096 - 财政年份:2015
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Molecular Interaction with Colloidal Surfaces Probed By Nonlinear Light Scattering
通过非线性光散射探测分子与胶体表面的相互作用
- 批准号:
1058883 - 财政年份:2011
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Nonlinear Optical Probe of Adsorption and Structure of Molecules on Nanometer and Micron Size Colloidal Particles
纳米和微米尺寸胶体颗粒吸附和分子结构的非线性光学探针
- 批准号:
0616836 - 财政年份:2007
- 资助金额:
$ 51.07万 - 项目类别:
Continuing Grant
Purchase of Departmental Beowulf Cluster for Computational Chemistry
购买计算化学系 Beowulf 集群
- 批准号:
0131132 - 财政年份:2002
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Inter- and Intra-Molecular Dynamics of Highly Vibrationally Excited Molecules
高振动激发分子的分子间和分子内动力学
- 批准号:
0111520 - 财政年份:2001
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Purchase of an X-ray Diffractometer Equipped with a CCD-Detector
购买配备 CCD 探测器的 X 射线衍射仪
- 批准号:
0091925 - 财政年份:2001
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Acquisition of a Time-Resolved Step Scan Fourier Transform Spectrometer
时间分辨步进扫描傅里叶变换光谱仪的采集
- 批准号:
0078181 - 财政年份:2000
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Teacher Enhancement in Chemistry: Creation of a Master in Chemical Education Program for Secondary School Teachers in Delaware, Maryland, New Jersey and Pennsylvania
化学教师提升:为特拉华州、马里兰州、新泽西州和宾夕法尼亚州的中学教师创建化学教育硕士项目
- 批准号:
9911825 - 财政年份:2000
- 资助金额:
$ 51.07万 - 项目类别:
Continuing Grant
Purchase of High-Performance Computing Environment for Research in Theoretical Biophysical Chemistry
购买高性能计算环境用于理论生物物理化学研究
- 批准号:
9816497 - 财政年份:1999
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Electronic and Vibrational Energy Transfer Dynamics Studied Through van der Waals Complexes
通过范德华配合物研究电子和振动能量传递动力学
- 批准号:
9626129 - 财政年份:1996
- 资助金额:
$ 51.07万 - 项目类别:
Continuing grant
相似海外基金
Breaking the Barrier: Mapping protein interactions in the bacterial outer membrane as targets for new antimicrobials
打破障碍:绘制细菌外膜中的蛋白质相互作用作为新抗菌药物的目标
- 批准号:
MR/Y012453/1 - 财政年份:2024
- 资助金额:
$ 51.07万 - 项目类别:
Research Grant
A cost-effective, high-throughput screening platform for modulator discovery of a-synuclein membrane interactions involved in neurodegenerative diseases
一种经济有效的高通量筛选平台,用于发现神经退行性疾病中涉及的α-突触核蛋白膜相互作用的调节剂
- 批准号:
10667047 - 财政年份:2023
- 资助金额:
$ 51.07万 - 项目类别:
EAGER: Development of a Fluorescent Reporter for Protein-Membrane Interactions
EAGER:开发蛋白质-膜相互作用的荧光报告基因
- 批准号:
2330643 - 财政年份:2023
- 资助金额:
$ 51.07万 - 项目类别:
Standard Grant
Advancing our knowledge of viral membrane fusion and of IDP-membrane interactions by ESR
通过 ESR 增进我们对病毒膜融合和 IDP-膜相互作用的了解
- 批准号:
10798605 - 财政年份:2023
- 资助金额:
$ 51.07万 - 项目类别:
Advancing our knowledge of viral membrane fusion and of IDP-membrane interactions by ESR
通过 ESR 增进我们对病毒膜融合和 IDP-膜相互作用的了解
- 批准号:
10552109 - 财政年份:2023
- 资助金额:
$ 51.07万 - 项目类别:
Probing the specific interactions of AlphaA- crystallin and its aging- and cataract-associated forms with lens cell membrane mimics
探讨 AlphaA-晶状体蛋白及其与衰老和白内障相关的形式与晶状体细胞膜模拟物的特定相互作用
- 批准号:
10667060 - 财政年份:2023
- 资助金额:
$ 51.07万 - 项目类别:
Theoretical assessment of the microscopic interactions as a key factor of the antifouling properties of membrane materials for water treatment
微观相互作用作为水处理膜材料防污性能关键因素的理论评估
- 批准号:
22K04802 - 财政年份:2022
- 资助金额:
$ 51.07万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Real-time single particle analysis of reovirus-membrane interactions that drive infection
驱动感染的呼肠孤病毒-膜相互作用的实时单粒子分析
- 批准号:
10516836 - 财政年份:2022
- 资助金额:
$ 51.07万 - 项目类别:
Dynamic Interactions between Intrinsically Disordered Proteins and Curved Membrane Surfaces
本质无序蛋白质与弯曲膜表面之间的动态相互作用
- 批准号:
10502133 - 财政年份:2022
- 资助金额:
$ 51.07万 - 项目类别:
Structure and function of mGluR3 interactions with beta-arrestins and the membrane.
mGluR3 与 β-arrestins 和膜相互作用的结构和功能。
- 批准号:
10704588 - 财政年份:2022
- 资助金额:
$ 51.07万 - 项目类别: