Antimicrobial-Membrane Interactions in Living Bacteria

活细菌中抗菌剂与膜的相互作用

基本信息

  • 批准号:
    2105387
  • 负责人:
  • 金额:
    $ 51.07万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-07-01 至 2024-06-30
  • 项目状态:
    已结题

项目摘要

With the support of the Chemistry of Life Processes (CLP) Program in the Division of Chemistry, Professor Hai-Lung Dai of Temple University is studying how bacterial membranes regulate their interactions with the surrounding environment, in particular those containing antimicrobial agents. The goal of this project is to provide fundamental knowledge that holds potential for future development of more effective antimicrobials that destroy disease-causing bacteria and reduce bacterial resistance to antibiotics. The role of small molecules and synthetic proteins in changing the permeability of bacterial cell membranes will be studied by quantitatively examining the response of small molecules whose interaction with light allows for their very sensitive detection when bound to cell membrane components. This powerful light-based method will be used to test how tiny holes are formed in the membrane upon membrane interaction with nearby chemicals or by changing how molecules move through channels in the membrane of bacteria. Professor Dai will integrate high school, college, and PhD students into the research program, providing a highly diverse group of students a very broad scientific skill set to help them pursue their career goals. Further broadening the impact of the project is a summer camp for Philadelphia inner city middle school students. In sum, the project will help strengthen the scientific workforce by training and exposing young minds to research aimed at providing answers to important fundamental questions about the chemistry of bacterial membranes with the ultimate goal of providing information key to addressing bacterial infections and antibiotic resistance. The significance of the research area is evidenced by the growing problem of antimicrobial-resistant bacterial infections, which are predicted by the World Health Organization and the United Nations to result in the death of 10 million people annually by 2050. In this study, Professor Dai will examine how the complex wall structures of bacteria exercise their regulatory functions on external molecules like antimicrobials. We will delineate, using both nonlinear optical spectroscopy and microscopy approaches, a variety of membrane transport mechanisms. These will include those based on import channels, efflux pumps, and lipid bilayer permeability, which play key roles in regulating intracellular concentrations of molecules critical to bacteria viability and protecting the bacteria from harmful compounds or an over-abundance of what would otherwise be thought to be useful compounds. The research will provide fundamental understanding of how antimicrobials interact with the various components of bacterial membranes to make them more permeable and reduce the protective functions of the membrane. Experiments will be designed to gather data critical for understanding how membranes perform their regulatory functions for living cells, as well as for designing strategies for developing more effective antimicrobials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在化学系生命过程化学(CLP)项目的支持下,天普大学戴海龙教授正在研究细菌膜如何调节它们与周围环境的相互作用,特别是那些含有抗菌剂的细菌。 该项目的目标是提供基础知识,为未来开发更有效的抗菌剂提供潜力,这些抗菌剂可以摧毁致病细菌并减少细菌对抗生素的耐药性。 小分子和合成蛋白质在改变细菌细胞膜的渗透性中的作用将通过定量检测小分子的反应来研究,小分子与光的相互作用使其在与细胞膜成分结合时能够非常灵敏地检测。 这种强大的基于光的方法将用于测试膜与附近化学物质相互作用时如何在膜中形成小孔,或者通过改变分子如何通过细菌膜中的通道移动。 戴教授将高中,大学和博士生融入研究计划,为高度多样化的学生群体提供非常广泛的科学技能,以帮助他们追求自己的职业目标。 进一步扩大该项目的影响是为费城内城中学生举办夏令营。 总而言之,该项目将通过培训和让年轻人参与旨在为细菌膜化学的重要基本问题提供答案的研究来帮助加强科学工作者,最终目标是提供解决细菌感染和抗生素耐药性的关键信息。 该研究领域的重要性体现在日益严重的耐药性细菌感染问题上,据世界卫生组织和联合国预测,到2050年,每年将导致1000万人死亡。在这项研究中,戴教授将研究细菌复杂的壁结构如何对抗菌剂等外部分子发挥调节作用。 我们将描绘,使用非线性光学光谱和显微镜的方法,各种膜运输机制。 这些将包括基于输入通道、外排泵和脂质双层渗透性的那些,其在调节对细菌活力至关重要的分子的细胞内浓度和保护细菌免受有害化合物或过量的原本被认为是有用的化合物的影响方面发挥关键作用。 这项研究将提供抗菌剂如何与细菌膜的各种成分相互作用的基本理解,使它们更具渗透性并降低膜的保护功能。实验将被设计为收集关键数据,以了解膜如何执行其对活细胞的调节功能,以及为开发更有效的抗菌剂设计策略。该奖项反映了NSF的法定使命,并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Hai-Lung Dai其他文献

Ultraviolet laser-induced formation of thin silicon oxide film from the precursor β-chloroethyl silsesquioxane
  • DOI:
    10.1557/jmr.1999.0132
  • 发表时间:
    2011-01-31
  • 期刊:
  • 影响因子:
    2.900
  • 作者:
    Jaya Sharma;Donald H. Berry;Russell J. Composto;Hai-Lung Dai
  • 通讯作者:
    Hai-Lung Dai
Time-Resolved Studies of Adsorption and Transport of a Hydrophobic Ion at Escherichia coli Bacterial Membranes by Second Harmonic Generation
  • DOI:
    10.1016/j.bpj.2008.12.654
  • 发表时间:
    2009-02-01
  • 期刊:
  • 影响因子:
  • 作者:
    Jia Zeng;Heather Eckenrode;Hai-Lung Dai
  • 通讯作者:
    Hai-Lung Dai
The effect of adsorption of Na on Cu(1 1 0) surface states probed by second harmonic generation
  • DOI:
    10.1016/j.susc.2005.03.047
  • 发表时间:
    2005-06-01
  • 期刊:
  • 影响因子:
  • 作者:
    Susan M. Dounce;Hai-Lung Dai
  • 通讯作者:
    Hai-Lung Dai
Aniline on Ag(1 1 1): Adsorption configuration, adsorbate–substrate bond, and inter-adsorbate interactions
  • DOI:
    10.1016/j.susc.2005.05.048
  • 发表时间:
    2005-09-01
  • 期刊:
  • 影响因子:
  • 作者:
    Thomas J. Rockey;Minchul Yang;Hai-Lung Dai
  • 通讯作者:
    Hai-Lung Dai
Carrier recombination of organic-inorganic 3D halide perovskite single crystals
有机-无机3D卤化物钙钛矿单晶的载流子复合
  • DOI:
    10.1063/1674-0068/cjcp1911197
  • 发表时间:
    2020-05
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Chao He;Xia Li;Yu-hao Wu;Hai-Lung Dai;Dong-feng Zhao;Yang Chen
  • 通讯作者:
    Yang Chen

Hai-Lung Dai的其他文献

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{{ truncateString('Hai-Lung Dai', 18)}}的其他基金

Nonlinear Light Scattering Spectroscopy and Microscopy of Molecular Interactions at Biological Surfaces
生物表面分子相互作用的非线性光散射光谱和显微镜
  • 批准号:
    1465096
  • 财政年份:
    2015
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Molecular Interaction with Colloidal Surfaces Probed By Nonlinear Light Scattering
通过非线性光散射探测分子与胶体表面的相互作用
  • 批准号:
    1058883
  • 财政年份:
    2011
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Nonlinear Optical Probe of Adsorption and Structure of Molecules on Nanometer and Micron Size Colloidal Particles
纳米和微米尺寸胶体颗粒吸附和分子结构的非线性光学探针
  • 批准号:
    0616836
  • 财政年份:
    2007
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Continuing Grant
Purchase of Departmental Beowulf Cluster for Computational Chemistry
购买计算化学系 Beowulf 集群
  • 批准号:
    0131132
  • 财政年份:
    2002
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Inter- and Intra-Molecular Dynamics of Highly Vibrationally Excited Molecules
高振动激发分子的分子间和分子内动力学
  • 批准号:
    0111520
  • 财政年份:
    2001
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Purchase of an X-ray Diffractometer Equipped with a CCD-Detector
购买配备 CCD 探测器的 X 射线衍射仪
  • 批准号:
    0091925
  • 财政年份:
    2001
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Acquisition of a Time-Resolved Step Scan Fourier Transform Spectrometer
时间分辨步进扫描傅里叶变换光谱仪的采集
  • 批准号:
    0078181
  • 财政年份:
    2000
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Teacher Enhancement in Chemistry: Creation of a Master in Chemical Education Program for Secondary School Teachers in Delaware, Maryland, New Jersey and Pennsylvania
化学教师提升:为特拉华州、马里兰州、新泽西州和宾夕法尼亚州的中学教师创建化学教育硕士项目
  • 批准号:
    9911825
  • 财政年份:
    2000
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Continuing Grant
Purchase of High-Performance Computing Environment for Research in Theoretical Biophysical Chemistry
购买高性能计算环境用于理论生物物理化学研究
  • 批准号:
    9816497
  • 财政年份:
    1999
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Standard Grant
Electronic and Vibrational Energy Transfer Dynamics Studied Through van der Waals Complexes
通过范德华配合物研究电子和振动能量传递动力学
  • 批准号:
    9626129
  • 财政年份:
    1996
  • 资助金额:
    $ 51.07万
  • 项目类别:
    Continuing grant

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Breaking the Barrier: Mapping protein interactions in the bacterial outer membrane as targets for new antimicrobials
打破障碍:绘制细菌外膜中的蛋白质相互作用作为新抗菌药物的目标
  • 批准号:
    MR/Y012453/1
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EAGER:开发蛋白质-膜相互作用的荧光报告基因
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通过 ESR 增进我们对病毒膜融合和 IDP-膜相互作用的了解
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