NSF Postdoctoral Fellowship in Biology FY 2021: Role of Transposable Elements and DNA Methylation on Immunity Gene Regulation and Diversification in Maize and Model Setaria viridis

2021 财年 NSF 生物学博士后奖学金：转座元件和 DNA 甲基化对玉米和模型狗尾草免疫基因调控和多样化的作用

• 批准号: 2109697
• 负责人: Andrew Read
• 金额: $ 21.6万
• 依托单位: Read, Andrew C
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2109697&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

This action funds an NSF Plant Genome Postdoctoral Research Fellowship in Biology for FY 2021. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The title of the research and training plan for this fellowship to Andrew Read is “The role of transposable elements and DNA methylation on immunity gene regulation and diversification in maize and the model monocot Setaria viridis.” The host institution for the fellowship is the University of Minnesota and the sponsoring scientist is Dr. Nathan Springer.Although plants are able to fend off many disease-causing organisms, when a pathogen successfully infects a plant the results can be devastating. Aggressive pathogens can result in loss of tree species in forests and crop loss in agricultural systems. This project will improve our understanding of how individual plants sense and respond to pathogens. Additionally, the research will examine the genetic features that allow plant populations to rapidly evolve diverse sets of immunity genes. Unlike much prior work in this field, this project will focus on plant immunity in the grasses, a group of plants that includes many of our most important staple crops such as rice, corn, and wheat. Altogether, this research will empower the breeding and development of improved disease resistance in these important crops. Each of the three research objectives generates a resource for the broader plant science community. Over the course of the project, the Fellow will receive technical and leadership skills from a group of interdisciplinary scientists and will in turn mentor several undergraduates during meaningful research experiences. Further, the Fellow will engage in community outreach via Market Science, a program led by post-docs and graduate students to provide family-friendly science education. Plants lack an adaptive immune system and, instead, encode a diverse suite of rapidly evolving immunity genes. It is becoming increasingly clear that transposable elements (TEs) and DNA methylation influence the evolution and expression of these immunity genes. Research on the model dicot Arabidopsis thaliana has been foundational in our understanding of TEs, DNA methylation, and immunity gene biology, however many of our most important crop plants are monocot grasses, a group that diverged from dicots over 125 million years ago. This project will combine a descriptive study of TE, DNA methylation, and immunity gene associations in a diverse maize population with mechanistic testing of these associations using CRISPR/Cas gene knockouts and transgenic stress-reporter lines of the model monocot Setaria viridis. These data and tools will enable a better understanding of monocot responses to biotic stress and inform the development and deployment of disease resistant cultivars. Results of research will be shared on pre-print servers when submitted to open-access scientific journals. Plasmids generated over the course of the project will be deposited with Addgene and transgenic and edited plants will be made available.Keywords: plant immunity, epigenetics, transposable elements, Setaria viridis, disease resistanceThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动为2021财年的NSF植物基因组博士后生物学研究奖学金提供资金。该研究金支持研究员在东道实验室的研究和培训计划，研究员还提出了扩大生物学参与的计划。该奖学金的研究和培训计划的标题为安德鲁·里德是“转座因子和DNA甲基化对玉米和模式单子叶植物狗尾草免疫基因调控和多样化的作用”。该研究金的主办机构是明尼苏达大学，赞助科学家是内森·斯普林格博士。尽管植物能够抵御许多致病生物，但当病原体成功感染植物时，结果可能是毁灭性的。侵袭性病原体可导致森林中树种的损失和农业系统中作物的损失。该项目将提高我们对单个植物如何感知和响应病原体的了解。此外，该研究还将研究允许植物种群快速进化出不同免疫基因组的遗传特征。与该领域的许多先前工作不同，该项目将重点关注草中的植物免疫，草是一组植物，包括许多我们最重要的主食作物，如水稻，玉米和小麦。总而言之，这项研究将使这些重要作物的育种和抗病性提高。这三个研究目标中的每一个都为更广泛的植物科学界提供了资源。在该项目的过程中，研究员将从一组跨学科科学家那里获得技术和领导技能，并在有意义的研究经验中指导几名本科生。此外，研究员将通过市场科学参与社区外展，这是一个由博士后和研究生领导的项目，旨在提供家庭友好的科学教育。 植物缺乏适应性免疫系统，而是编码一套快速进化的免疫基因。转座因子（transposable elements，TE）和DNA甲基化对这些免疫基因的进化和表达的影响越来越明显。对模式双子叶植物拟南芥的研究是我们理解TE、DNA甲基化和免疫基因生物学的基础，然而我们许多最重要的作物是单子叶植物，这是一个在1.25亿年前从双子叶植物分化出来的群体。该项目将联合收割机结合不同玉米群体中TE，DNA甲基化和免疫基因关联的描述性研究，以及使用CRISPR/Cas基因敲除和模式单子叶植物狗尾草的转基因应激报告细胞系对这些关联进行的机制测试。这些数据和工具将有助于更好地了解单子叶植物对生物胁迫的反应，并为抗病品种的开发和部署提供信息。研究结果将在提交给开放获取的科学期刊时在预印本服务器上共享。关键词：植物免疫，表观遗传学，转座因子，狗尾草，抗病性该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。