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NSF Postdoctoral Fellowship in Biology FY 2021: Parallel genome and phenome evolution within and across fish species

2021 财年 NSF 生物学博士后奖学金：鱼类内部和之间的平行基因组和表型进化

基本信息

批准号：
2109761
负责人：
Daniel MacGuigan
金额：
$ 13.8万
依托单位：
MacGuigan, Daniel J
依托单位国家：
美国
项目类别：
Fellowship Award
财政年份：
2021
资助国家：
美国
起止时间：
2021-12-01 至 2024-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2109761&HistoricalAwards=false
关键词：
NSF Postdoctoral Fellowship Biology FY

项目摘要

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2021, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment and Phenotypes. The fellowship supports research and training of the Fellow that will contribute to the area of Rules of Life in innovative ways. A fundamental principle in biology is that under similar conditions, parallel evolution may lead to similar outcomes. Parallel evolution can occur among closely related populations within a single species and across distantly related species separated by millions of years of divergence. This research will focus on parallel evolution in the fish fauna of the Carolina bays, an understudied series of freshwater lakes along the Atlantic coastal plain. These lakes harbor several unique species plus replicate populations of the same species, providing a natural experiment to compare parallel evolution among versus within species. This research seeks to understand how species and populations have repeatedly evolved elongated bodies compared to their stream-dwelling closest relatives. Many previous studies of parallel evolution focus on detailed investigation of a single species or broad comparative approaches across many different species. This interdisciplinary project will compare parallel evolution within and among species across multiple levels of biological organization. In addition to advancing understanding of parallel evolution, this research will provide crucial information about genetic population structure for conservation of these species. The Fellow will develop a community outreach program to expand public awareness of freshwater ecosystem conservation as well as involving students of diverse backgrounds in the study.This proposal will test the hypothesis that parallel genomic changes drive parallel morphological divergence both within and across species. The Fellow will (1) investigate the phylogenetic and population genetic context for each replicate lake species/population, (2) determine the degree and magnitude of parallel phenotypic lake-stream divergence, and (3) examine parallel genomic evolution linked to phenotypic divergence. This research will generate a uniquely rich dataset to study parallel evolution by combining de novo reference genome sequences, population and species low-coverage genome resequencing, traditional 2D morphometrics, 3D osteological morphometrics using µCT imaging, and experimental gene-editing in Danio. Training in these diverse research techniques will allow the Fellow to build on their current skill set and expand into new areas of scientific inquiry. The Fellow will mentor undergraduate students in collaboration with the UB Women in STEM Cooperative and the SUNY Louis Stokes Alliance for Minority Participation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动资助了2021财年的NSF生物学博士后研究奖学金，即调查基因组，环境和表型之间相互作用的生命规则的综合研究。该研究金支持研究员的研究和培训，以创新的方式为生活规则领域做出贡献。生物学的一个基本原则是，在相似的条件下，平行进化可能导致相似的结果。平行进化可以发生在同一个物种内的密切相关的种群之间，也可以发生在数百万年来分离的远亲物种之间。这项研究将集中在平行进化的鱼类动物群的卡罗莱纳湾，一个欠研究的系列淡水湖沿着大西洋沿岸平原。这些湖泊拥有几个独特的物种，加上相同物种的复制种群，提供了一个自然的实验来比较物种之间和物种内部的平行进化。这项研究旨在了解物种和种群如何与它们的河流近亲相比反复进化出细长的身体。许多以前的平行进化研究都集中在对单个物种的详细调查或对许多不同物种的广泛比较方法上。这个跨学科的项目将在生物组织的多个层次上比较物种内部和物种之间的平行进化。除了推进对平行进化的理解外，这项研究还将为保护这些物种提供有关遗传种群结构的重要信息。该研究员将开发一个社区外展计划，以扩大公众对淡水生态系统保护的认识，并让不同背景的学生参与研究。该提案将测试平行基因组变化驱动物种内和物种间平行形态差异的假设。研究员将（1）调查每个复制湖泊物种/种群的系统发育和种群遗传背景，（2）确定平行表型湖流分歧的程度和幅度，以及（3）检查与表型分歧相关的平行基因组进化。这项研究将产生一个独特的丰富的数据集，通过结合从头参考基因组序列，人口和物种低覆盖率基因组重测序，传统的2D形态测量学，使用µCT成像的3D骨骼形态测量学以及Danio的实验基因编辑来研究平行进化。在这些不同的研究技术的培训将使研究员建立在他们目前的技能，并扩大到科学探究的新领域。该研究员将与UB Women in STEM Cooperative和SUNY Louis Stokes Alliance for Minority Participation合作指导本科生。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。