NSF Postdoctoral Fellowship in Biology FY 2021: Replaying the tape of Genotype-Phenotype Evolution: Integrating Evidence of Climbing Habit Evolution across Alstroemeriaceae

本行动资助2021财年美国国家科学基金会植物基因组生物学博士后研究奖学金。该奖学金支持奖学金获得者在主办实验室的研究和培训计划，该奖学金获得者还提出了扩大生物学参与的计划。这个奖学金给Carrie M. Tribble的研究和培训计划的标题是“重放基因型-表型进化的磁带：整合Alstroemeriaceae攀爬习惯进化的遗传，解剖和系统发育证据”。该奖学金的主办机构是夏威夷大学马诺阿分校和Antioquia大学（哥伦比亚），赞助科学家是Rosana Zenil-Ferguson博士和Fernando博士Alzate-Guarín。不同的物种偶尔会独立地进化出相同的特征。这一过程被称为趋同进化，可能发生在距离较远或关系较近的物种中，可能是由于潜在DNA中不同或相似的变化。通常情况下，趋同进化发生在不同物种对相似环境的反应中。为了更好地理解趋同进化发生的方式和原因，本研究将收集攀缘藤趋同进化的数据，并模拟植物生长环境的变化和DNA的修饰如何导致共享的、独立进化的特征的进化。培训目标包括掌握数学建模、实地考察、植物解剖和系统学方面的新技能。通过该奖学金的支持将为研究员提供新的机会，通过两个教育项目培训学生：哥伦比亚合作机构的西班牙语本科生和研究生方法课程和夏威夷大学里昂植物园的高中学生教育植物学实习项目。趋同和平行进化已经产生了一些环境压力驱动表型的最具代表性的例子，包括远亲多肉植物令人难以置信的相似性，脊椎动物和无脊椎动物之间非凡的飞行能力，以及尽管共同祖先缺乏耐药性，但细菌中抗生素耐药性的新出现。然而，尽管这些过程在产生许多观察到的表型变异中很重要，但对产生重复表型的基因组和环境因素的了解仍然缺乏。形态学水平上的“趋同”在多大程度上与解剖学或分子水平上的趋同相匹配，目前尚不清楚。为了更好地理解重复表型是如何产生的，该项目将包括对一个植物科——Alstroemeriaceae——的详细研究，该植物科具有明显的攀缘习惯变异、重复表型和开花植物的关键创新。通过三个目标，本研究将详细描述这种习惯的环境、形态、解剖和遗传基础，并通过以下方式验证共同的遗传、环境和解剖变化对攀登进化负责的假设：(1)建立一种新的模型来识别重复表型进化的分子基础；(2)模拟了攀援植物和非攀援植物的茎解剖和环境演化；(3)实施新模型，鉴定桤木科植物攀援的遗传基础。本项目产生的数据包括：(1)野外测量的环境变量，(2)标本馆记录，(3)发育序列的茎解剖描述，(4)Alstroemeriaceae的进化关系（系统发生），(5)基因组水平的基因序列（RADSeq区域），以及(6)Luzuriaga radicans的全基因组。所有数据将通过NCBI、TreeBASE和data Dryad等在线数据库公开提供。关键词：趋同进化，攀爬，Alstroemeriaceae，系统发育比较方法该奖项反映了美国国家科学基金会的法定使命，并通过基金会的知识价值和更广泛的影响审查标准进行了评估，认为值得支持。

This action funds an NSF Plant Genome Postdoctoral Research Fellowship in Biology for FY 2021. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The title of the research and training plan for this fellowship to Carrie M. Tribble is "Replaying the tape of Genotype-Phenotype Evolution: Integrating Genetic, Anatomical, and Phylogenetic Evidence of Climbing Habit Evolution across Alstroemeriaceae" The host institution for the fellowship is the University of Hawai`i at Manoa and the Universidad de Antioquia (Colombia) and the sponsoring scientists are Dr. Rosana Zenil-Ferguson and Dr. Fernando Alzate-Guarín.Different species occasionally evolve the same feature independently. This process, called convergent evolution, can occur in distantly or closely related species and may be due to different or similar changes in the underlying DNA. Often, convergent evolution occurs when distinct species evolve in response to similar environments. To better understand how and why convergent evolution occurs, this research will gather data on the convergent evolution of climbing vines and model how changes in the environment where plants grow and modifications to DNA can lead to the evolution of shared, independently evolved features. Training objectives include acquiring new skills in mathematical modeling, fieldwork, plant anatomy and systematics. Support through this fellowship will provide new opportunities for the Fellow to train students through two educational programs: a Spanish-language undergraduate and graduate student methods course at partner institutions in Colombia and an educational botany internship program for high school students at Lyon Arboretum at the University of Hawai`i. Convergent and parallel evolution have generated some of the most iconic examples of environmental pressures driving phenotypes, including the mind-boggling similarity of distantly related succulent plants, the extraordinary ability to fly among vertebrate and invertebrate animals, and the novel emergence of antibiotic-resistance in bacteria despite the lack of resistance in common ancestors. Yet, despite the importance of these processes in producing much of the observed phenotypic variation, knowledge of the genomic and environmental factors that generate repeated phenotypes is lacking. It is still unknown to what extent “convergence” at the morphological level is matched by convergence at the anatomical or molecular level. To better understand how repeated phenotypes are produced, the project will include detailed studies on a botanical family – Alstroemeriaceae – with clear variation in the climbing habit, a repeated phenotype and key innovation in flowering plants. Through three aims, this research will characterize the detailed environmental, morphological, anatomical, and genetic underpinnings of this habit and test the hypothesis that shared genetic, environmental, and anatomical changes are responsible for the evolution of climbing by: (1) developing a novel model for identifying the molecular basis of repeated phenotypic evolution; (2) modeling the evolution of stem anatomy and environments of climbing and non-climbing plants; and (3) implementing the new model to identify the genetic underpinnings of climbing in Alstroemeriaceae. The data generated by this project include (1) field-measured environmental variables, (2) herbarium vouchers, (3) stem anatomy descriptions over a developmental series, (4) evolutionary relationships (phylogenies) of Alstroemeriaceae, (5) genome-level genetic sequences (RADSeq regions), and (6) a whole genome of Luzuriaga radicans. All data will be made publicly available through online data repositories such as NCBI, TreeBASE, and Data Dryad. Keywords: convergent evolution, climbing, Alstroemeriaceae, phylogenetic comparative methodsThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.