MRI: Acquisition of a CytoViva enhanced microscope with hyperspectral imaging capability for multidisciplinary research and education in nanotechnology

MRI：购买具有高光谱成像功能的 CytoViva 增强型显微镜，用于纳米技术的多学科研究和教育

• 批准号: 2116140
• 负责人: Nasim Nosoudi
• 金额: $ 26.77万
• 依托单位: Marshall University Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2116140&HistoricalAwards=false
• 关键词: MRI; Acquisition; CytoViva; enhanced; microscope

Nanoscience and technology are playing an increasing role in fundamental research related to the engineering (e.g., mechanical, biomedical) and scientific (e.g., chemistry, biology) disciplines. To be able to observe and control phenomena at nanoscale, researchers need to be equipped with appropriate tools. This award will facilitate the acquisition of a specialized microscope used to visualize samples at very small dimensions. Students and faculty at Marshall University will use this microscope to gain fundamental knowledge in a range of important research projects. These include investigating calcium deposit in blood vessels, detecting toxic nanoparticles in human cells, water treatment to remove contamination, and in pharmaceutical manufacturing. This cutting-edge instrument will significantly advance the academic careers and research output of at least five faculty members. More than 100 mostly underrepresented students will be trained, under faculty mentorship, in modern engineering and scientific disciplines both during lectures, laboratory modules, and demonstrations. The incorporation of nanoscience and technology in the undergraduate and graduate curriculum will help training the future workforce, especially in the growing biotechnology arena in West Virginia.This award enables Marshall University to purchase a CytoViva enhanced, high signal-to-noise darkfield optical microscope with an integrated hyperspectral imaging system. This instrument will be used to address fundamental knowledge gaps in highly multidisciplinary nanoscience and nanotechnology research projects at the university. The microscope produces a very high signal-to-noise ratio, enabling scatter detection of nanoscale materials at up to ten times greater than standard darkfield optics. The technology can image label-free nanoparticles and requires no sample preparation. Label-free nanoscale materials can then be spectrally identified based on their unique spectral properties within their target environment. The microscope will enable Marshall faculty in biomedical and mechanical science and engineering to accelerate their research contributions and improve teaching through innovative laboratory demonstrations and the integration of research and pedagogy. Researchers will use the instrument’s capability to examine phenomena such as cell differentiation, understanding the environmental exposure to silver nanoparticles, label-free quantitation of short nucleic acids using Surface immobilized plasmonic sensors for RNA, nanoparticle aggregation in water treatment, and Brownian motion and aggregation of nanofluids.This project is jointly funded by the Major Research Instrumentation Program and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纳米科学和技术在与工程(如机械、生物医学)和科学(如化学、生物)学科相关的基础研究中发挥着越来越大的作用。为了能够在纳米尺度上观察和控制现象，研究人员需要配备适当的工具。这项合同将有助于采购一种专门的显微镜，用于观察非常小尺寸的样品。马歇尔大学的学生和教职员工将使用这种显微镜在一系列重要的研究项目中获得基础知识。这些措施包括调查血管中的钙沉积，检测人体细胞中的有毒纳米颗粒，水处理以去除污染，以及在制药方面。这一尖端工具将极大地促进至少五名教职员工的学术生涯和研究成果。100多名学生将在教师的指导下，在讲座、实验室模块和演示中接受现代工程和科学学科的培训。将纳米科学和技术纳入本科和研究生课程将有助于培训未来的劳动力，特别是在西弗吉尼亚州不断增长的生物技术领域。该奖项使马歇尔大学能够购买具有集成高光谱成像系统的Cytoviva增强型高信噪比暗场光学显微镜。该仪器将用于解决该大学高度多学科的纳米科学和纳米技术研究项目中的基本知识差距。该显微镜产生非常高的信噪比，能够以高达标准暗场光学系统十倍的速度对纳米材料进行散射检测。这项技术可以成像无标签的纳米颗粒，不需要样品准备。然后，无标签纳米材料可以根据其目标环境中的独特光谱特性进行光谱识别。该显微镜将使生物医学、机械科学和工程方面的马歇尔教师能够通过创新的实验室演示以及研究和教学的整合，加快他们的研究贡献，并改善教学。研究人员将利用该仪器的能力来检查细胞分化、了解环境中银纳米粒子的暴露、使用表面固定的等离子体传感器进行短核酸的无标记量化、水处理中的纳米粒子聚集以及纳米流体的布朗运动和聚集。该项目由重大研究仪器计划和既定的刺激竞争研究计划(EPSCoR)联合资助。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Effect of ellagic acid and retinoic acid on collagen and elastin production by human dermal fibroblasts

鞣花酸和视黄酸对人真皮成纤维细胞产生胶原蛋白和弹性蛋白的影响

• DOI: 10.3233/bme-230007
• 发表时间: 2023
• 期刊: Bio-Medical Materials and Engineering
• 影响因子: 1
• 作者: Duckworth, Chloe;Stutts, Jada;Clatterbuck, Kayla;Nosoudi, Nasim
• 通讯作者: Nosoudi, Nasim