MRI: Acquisition of an HPLC Coupled Mass Spectrometer for proteomics, metabolomics, and small molecule applications to expand and upgrade the SJSU PROTEIN Lab

MRI：购买用于蛋白质组学、代谢组学和小分子应用的 HPLC 耦合质谱仪，以扩展和升级 SJSU 蛋白质实验室

• 批准号: 2116424
• 负责人: Annalise Van Wyngarden
• 金额: $ 77.22万
• 依托单位: San Jose State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2116424&HistoricalAwards=false
• 关键词: MRI; Acquisition; HPLC; Coupled; Mass

An award is made to San José State University (SJSU) to acquire a new HPLC-Mass Spectrometer instrument with improved metabolomics, proteomics and high-resolution small molecule capabilities for the SJSU core PROTEIN lab facility. San Jose State University is a federally recognized minority-serving institution. This instrument will benefit both current and future faculty across multiple disciplines by expanding the research capabilities at SJSU. The broader impacts of this proposal are to stimulate new research activities and strengthen collaboration among research faculty as well as continuing to train undergraduate and masters students and expose them to a state-of-the-art instrumentation in both classroom and research settings. Workshops will be developed to increase the number of faculty that will use the instrument in their research. A course in Chemical Biology will be revamped with a focus on the new instrument to provide valuable research and learning opportunities to students who are underrepresented in STEM fields. The intellectual merit of the supported research will (1) characterize how compounds found in fruits in vegetables play roles in cellular metabolism, (2) identify the molecular mechanisms in mammary gland development, (3) characterize bacteria that are able to metabolize and use rare earth elements for growth, (4) characterize the mechanism of action of combination therapies on inhibiting P. aeruginosa biofilm development, (5) understand how cellular pH plays a role in cellular processes including cell death, differentiation, and tumorigenesis, and (6) characterize small molecule development using a novel p450 biocatalysis platform.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向圣何塞州立大学（SJSU）颁发了奖励，以获取一种新的HPLC-MAS光谱仪仪器，具有改进的代谢组学，蛋白质组学和高分辨率小分子功能，以实现SJSU核心蛋白质实验室的功能。圣何塞州立大学是一家联邦认可的少数民族服务机构。该工具将通过扩大SJSU的研究能力来使当前和未来的教职员工受益。该建议的更广泛的影响是刺激新的研究活动并加强研究教师之间的合作，并继续培训本科生和掌握学生，并将其暴露于课堂和研究环境中的最先进的仪器中。将开发研讨会，以增加将在其研究中使用该工具的教师数量。化学生物学课程将进行改进，重点关注新工具，为在STEM领域中人数不足的学生提供宝贵的研究和学习机会。受支持的研究的智力优点（1）表征蔬菜中水果中发现的化合物在细胞代谢中的作用，（2）确定乳腺发育中的分子机制，（3）能够代谢和使用稀土元素来代谢和使用稀土元素的生长，（4）在生长中使用稀土元素，（4）在生长中的开发，（4）燃料的动作，（4），（4）均具有组合性，（4）了解细胞pH在包括细胞死亡，分化和肿瘤发生在内的细胞过程中如何发挥作用，以及（6）使用新型的P450生物催化平台来表征小分子的发育。该奖项反映了NSF的法定任务，并被认为是通过评估基金会的智力和更广泛的影响来通过评估来获得支持的珍贵的，这是珍贵的。