MRI: Acquisition of a Normal Phase Single Quadrupole High Performance Liquid Chromatography Mass Spectrometer (HPLC-MS) for Innovative Paleoclimate and Paleoenvironmental Research

MRI：购买正相单四极杆高效液相色谱质谱仪 (HPLC-MS)，用于创新古气候和古环境研究

• 批准号: 2117527
• 负责人: Melissa Berke
• 金额: $ 20.18万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117527&HistoricalAwards=false
• 关键词: MRI; Acquisition; Normal; Phase; Single

The acquisition of a high performance liquid chromatography-mass spectrometer (HPLC-MS) will support environmental research and educational activities led by Professor Melissa Berke and colleagues Adrian Rocha and Jason McLachlan at the University of Notre Dame. The HPLC-MS will be configured for state-of-the-art microbial membrane lipid analysis, optimized for the best reproducibility and resolution, innovations that have significantly changed the fields of paleoclimatology and paleoecology. HPLC-MS has propelled the growth of rapid, sensitive, and more economical detection of compounds that are harder to measure using earlier methods and instrumentation. The acquisition of this instrument strengthens the research infrastructure at the university and within the Midwest and in addition promotes multi-disciplinary collaborations. The instrument will help broaden participation by involving undergraduate and graduate students from diverse backgrounds who can use this analytical tool and resulting proxies in environmental change research. The new instrument will be available for use by other researchers at Notre Dame and beyond. The award supports the acquisition a new high performance liquid chromatography-mass spectrometer (HPLC-MS) for measurement of microbial membrane lipids, glycerol dialkyl glycerol tetraethers (GDGTs) at the University of Notre Dame. The Single Quad HPLC-MS will run in normal phase with solvent eluents, have an atmospheric pressure chemical ionization (APCI) source, a thermostatted column compartment that can fit multiple columns in tandem, a fraction collector for preparative mode, and an autosampler. Improvements in HPLC-MS have propelled the growth of rapid, sensitive, and more economical detection of higher molecular weight and polar compounds. Further, recent advances in microbial ecology and organic geochemistry have expanded our understanding of microbial lipid synthesis and spatial distribution, particularly with respect to membrane lipids of Bacteria and Archaea, known as GDGTs, some of the most ubiquitous lipids on Earth. Together, these innovations in analytical chemistry and understanding of microbial processes have led to proxy development using GDGT distributions and abundances to revolutionize research pertaining to changing environmental conditions, critical to understanding paleoclimatology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

高效液相色谱-质谱仪（HPLC-MS）的收购将支持由Melissa Berke教授及其同事Adrian罗查和Jason McLachlan领导的环境研究和教育活动。HPLC-MS将被配置为最先进的微生物膜脂质分析，优化最佳的再现性和分辨率，这些创新已经显著改变了古气候学和古生态学领域。HPLC-MS已经推动了快速，灵敏和更经济的化合物检测的发展，这些化合物很难使用早期的方法和仪器进行测量。该仪器的收购加强了大学和中西部的研究基础设施，并促进了多学科合作。该工具将有助于扩大参与，让来自不同背景的本科生和研究生参与，他们可以在环境变化研究中使用这一分析工具和由此产生的替代物。新仪器将可供圣母院及其他地方的其他研究人员使用。该奖项支持在圣母大学收购一种新的高效液相色谱-质谱仪（HPLC-MS），用于测量微生物膜脂质，甘油二烷基甘油四醚（GDGT）。Single Quad HPLC-MS将使用溶剂洗脱液在正相中运行，具有大气压化学电离（APCI）源、可串联安装多个色谱柱的恒温色谱柱室、用于制备模式的馏分收集器和自动进样器。HPLC-MS的改进推动了更高分子量和极性化合物的快速，灵敏和更经济检测的发展。此外，微生物生态学和有机地球化学的最新进展扩大了我们对微生物脂质合成和空间分布的理解，特别是关于细菌和细菌的膜脂质，称为GDGT，地球上最普遍的脂质。总之，这些创新在分析化学和微生物过程的理解，导致代理开发使用GDGT分布和丰度革命性的研究有关不断变化的环境条件，了解古气候学至关重要。这个奖项反映了NSF的法定使命，并已被认为是值得通过评估使用基金会的智力价值和更广泛的影响审查标准的支持。