H2O2 perception and signal transduction: the specific role of AHK5 (B05)

解决AHK5蛋白不同结构域的结构，并将结构信息与功能研究相结合。这将有助于了解AHK5在体外和体内如何感知H2O2或氧化还原条件。AHK5突变体的实验将证明组氨酸激酶活性、磷酸化能力和经典Ser / Thr / Tyr磷酸化途径中的串音在AHK5特异性植物应答中所必需的程度。此外，我们计划在H2O2作为信号分子的背景下，鉴定其他有助于ahk5特异性信号传导和功能的因素。

The structures of different AHK5 protein domains are to be solved and structural information combined with functional studies. This wil help to understand how the AHK5 peceives H2O2 or redox conditions in vitro and in vivo. Experiments with AHK5 mutants will demonstrate the extent to which histidine kinase activity, phosphorylay capacity, and cross-talk in classical Ser / Thr / Tyr phosphorylation pathways are required for the AHK5-specific plant responses. In addition, the identification of other factors contributing to AHK5-specific signaling and function in the context of H2O2 as a signaling molecule is planned.