EAGER: An innovative approach for quantification and prospective isolation of Nosema ceranae life stages from host cells with potential for application to diverse pathogen species
EAGER:一种从宿主细胞中定量和前瞻性分离蜜蜂微孢子虫生命阶段的创新方法,具有应用于多种病原体物种的潜力
基本信息
- 批准号:2125981
- 负责人:
- 金额:$ 9.71万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Some microbial pathogens are obligate intracellular pathogens. They are hard to study because of their complex life cycles which occur inside the cells of their hosts. In other areas of biology, a technique called fluorescence activated cell sorting (FACS) is used to identify and separate different cell types from a complex mixture to near homogeneity based on differential fluorescent-based staining. This proposal will use this technique to measure and isolate different life stages of a specific parasite (Nosema ceranae) that infects honey bees and negatively impacts colony health. In the work funded by this proposal, use of this technique is expected to increase the understanding of the course of infection in honey bees. This new system can also be used to increase knowledge of the biology of other intracellular pathogens in bees or other animals. AS such, this project serves the NSF mission to promote the progress of science. In turn, findings from using this technique can be the first step in identifying means to cure or prevent infections. Thus, this technology can be used in the bioeconomy by researchers who want to stabilize honey bee populations and pollination.The study of obligate intracellular pathogens is difficult because they cannot easily be reproduced outside the host in axenic conditions. Many avenues of inquiry necessitate that cells of the pathogen be separated from those of the host. In addition, to study intracellular pathogen species with complex life cycles, it is critical individual life stages can also be separated. The experimental challenges of such manipulations and separations are compounded in situations where both host and pathogen are non-model organisms for which there are limited or no species-specific molecular tools. The obstacles presented can be addressed using flow cytometry-based techniques for relative quantification and localization of different cell types from honey bees that contain different life stages of the Nosema ceranae microsporidian, a key pathogen of honey bees. Specialized cell dyes measuring general cell features, in conjunction with flow cytometry to isolate distinct cell populations, are expected to develop a new system to advance the understanding of host-pathogen interactions and infection dynamics. Establishment of this technique promises to facilitate a number of new directions in microsporidia research. As some of the cellular features assessed by this technique are widespread in eukaryotes, there is a high probability that the technique can be adapted for use with other pathogen species from diverse phylogenetic groups alone or in combination with other available dyes. This proposal will also train an undergraduate student in microbiology research. As such, this proposal is training the next generation of the science workforce.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
有些微生物病原体是专性细胞内病原体。它们很难研究,因为它们在宿主细胞内发生复杂的生命周期。在生物学的其他领域,一种称为荧光活化细胞分选(FACS)的技术被用于识别和分离不同的细胞类型,从复杂的混合物到接近均匀的基于差异荧光染色。该提案将使用该技术来测量和分离感染蜜蜂并对蜂群健康产生负面影响的特定寄生虫(微孔虫)的不同生命阶段。在这项提案资助的工作中,使用这种技术有望增加对蜜蜂感染过程的理解。这个新系统也可以用来增加对蜜蜂或其他动物的其他细胞内病原体的生物学知识。因此,这个项目服务于NSF促进科学进步的使命。反过来,使用这种技术的发现可以成为确定治疗或预防感染方法的第一步。因此,这项技术可以被那些想要稳定蜜蜂种群和授粉的研究人员用于生物经济。专性细胞内病原体的研究是困难的,因为它们在无菌条件下不容易在宿主外繁殖。许多研究途径都需要将病原体的细胞与宿主的细胞分开。此外,研究具有复杂生命周期的胞内病原菌种,也可以对关键的个体生命阶段进行分离。在宿主和病原体都是非模式生物的情况下,这种操作和分离的实验挑战更加复杂,因为只有有限的或没有特定物种的分子工具。这些障碍可以通过基于流式细胞术的技术来解决,该技术可以相对定量和定位蜜蜂不同类型的细胞,这些细胞含有蜜蜂的关键病原体微孢子虫(Nosema ceranae microsporidian)的不同生命阶段。专门的细胞染料测量一般细胞特征,结合流式细胞术分离不同的细胞群,有望开发一个新的系统,以促进对宿主-病原体相互作用和感染动力学的理解。这项技术的建立有望促进微孢子虫研究的一些新方向。由于该技术评估的一些细胞特征在真核生物中广泛存在,因此该技术很有可能适用于来自不同系统发育群的其他病原体物种单独使用或与其他可用染料结合使用。这一建议还将培养一名微生物学研究方面的本科生。因此,这一提议正在培养下一代科学工作者。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jonathan Snow其他文献
A new tectonic window into the backarc basin lower oceanic crust and upper mantle: Mado Megamullion in the Shikoku Basin
弧后盆地下洋壳和上地幔的新构造窗口:四国盆地的 Mado Megamullion
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Yasuhiko Ohara;Kyoko Okino;Norikatsu Akizawa;Masakazu Fujii;Yumiko Harigane;Naoto Hirano;Ken-ichi Hirauchi;Shiki Machida;Katsuyoshi Michibayashi;Alessio Sanfilippo;Jonathan Snow;Hiroyuki Yamashita;YK18-07 & KH18-2 scientists - 通讯作者:
YK18-07 & KH18-2 scientists
ギブス自由エネルギー最小化による反応帯形成のフォーワードモデル
吉布斯自由能最小化反应区形成的正演模型
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Yasuhiko Ohara;Kyoko Okino;Norikatsu Akizawa;Masakazu Fujii;Yumiko Harigane;Naoto Hirano;Ken-ichi Hirauchi;Shiki Machida;Katsuyoshi Michibayashi;Alessio Sanfilippo;Jonathan Snow;Hiroyuki Yamashita;YK18-07 & KH18-2 scientists;宇野正起 - 通讯作者:
宇野正起
Jonathan Snow的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jonathan Snow', 18)}}的其他基金
OSIB:RUI: Elucidating the cell biology and developmental regulation of sporogenesis and spore dimorphism in the microsporidia Nosema ceranae using a novel flow cytometry approach
OSIB:RUI:使用新型流式细胞术方法阐明微孢子虫微孢子虫孢子发生和孢子二态性的细胞生物学和发育调控
- 批准号:
2243451 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Collaborative Research: RUI: A multiscale quantification of plasmid acquisition in Escherichia coli pathogens
合作研究:RUI:大肠杆菌病原体中质粒获取的多尺度定量
- 批准号:
2040697 - 财政年份:2021
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Collaborative Research: Using Osmium-Lead isotope variations in mid-ocean ridge and abyssal peridotite sulfides to understand fundamental properties of Earth's mantle
合作研究:利用大洋中脊和深海橄榄岩硫化物中的锇铅同位素变化来了解地幔的基本特性
- 批准号:
1737031 - 财政年份:2017
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Death of a Backarc Rift: A Petrologic Site Survey of Godzilla Mullion
弧后裂谷之死:哥斯拉竖框的岩石学现场调查
- 批准号:
1030950 - 财政年份:2010
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Collaborative Research: Geochemistry and Tectonics of Cretaceous Gateway Closure in the Central American Isthmus
合作研究:中美洲地峡白垩纪门户闭合的地球化学和构造
- 批准号:
1019382 - 财政年份:2010
- 资助金额:
$ 9.71万 - 项目类别:
Continuing Grant
SGER: IODP Site Survey participation YK-0905, Tectonic and petrologic evolution of Godzilla Mullion
SGER:IODP 现场调查参与 YK-0905,哥斯拉竖框的构造和岩石演化
- 批准号:
0914708 - 财政年份:2009
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Collaborative Research: Os, Nd and Hf isotopes in abyssal peridotites from Arctic Lena Trough: Continental lithospheric or asthenospheric mantle origin?
合作研究:北极勒拿海槽深海橄榄岩中的 Os、Nd 和 Hf 同位素:大陆岩石圈或软流圈地幔起源?
- 批准号:
0648567 - 财政年份:2007
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
相似海外基金
NSF Convergence Accelerator (L): Innovative approach to monitor methane emissions from livestock using an advanced gravimetric microsensor.
NSF Convergence Accelerator (L):使用先进的重力微传感器监测牲畜甲烷排放的创新方法。
- 批准号:
2344426 - 财政年份:2024
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Health System Science: An Innovative Approach to Addressing Chronic Disease Management in Medical Education
卫生系统科学:解决医学教育中慢性病管理的创新方法
- 批准号:
502606 - 财政年份:2024
- 资助金额:
$ 9.71万 - 项目类别:
Exploring ATR and PARP Inhibitors for Ovarian Cancer Management: An Innovative Approach
探索 ATR 和 PARP 抑制剂用于卵巢癌治疗:一种创新方法
- 批准号:
494635 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Operating Grants
High-Throughput Functional Genomics of Variants in Genes Linked to Substance Use Disorders
与药物使用障碍相关的基因变异的高通量功能基因组学
- 批准号:
10728785 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
An innovative and straightforward approach to construct and manipulate viral infectious clones
构建和操作病毒感染性克隆的创新且简单的方法
- 批准号:
10667766 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Biology through art: an innovative, interdisciplinary approach to teaching biology
通过艺术的生物学:一种创新的、跨学科的生物学教学方法
- 批准号:
2315749 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Standard Grant
Modernization of 3-dimensional printing capabilities at the Aquatic Germplasm and Genetic Resource Center
水产种质和遗传资源中心 3 维打印能力的现代化
- 批准号:
10736961 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Evaluating an Innovative Approach to Understanding the Development of Walking and Care for Children with Mobility Impairments
评估了解步行发展和照顾行动障碍儿童的创新方法
- 批准号:
495766 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Operating Grants
Combination of tumor targeted therapy with stroma modulating agent for PDAC
肿瘤靶向治疗与基质调节剂联合治疗 PDAC
- 批准号:
10629924 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别:
Fibroblast-derived laminin regulates blood-brain barrier integrity and fibroblast biology in hemorrhagic brain
成纤维细胞衍生的层粘连蛋白调节出血脑中的血脑屏障完整性和成纤维细胞生物学
- 批准号:
10749280 - 财政年份:2023
- 资助金额:
$ 9.71万 - 项目类别: