RUI: Microelectrophoretic and Statistical Tools for Studies of Kinase- and ROS-Based Signaling in Dictyostelium discoideum

RUI：用于研究盘基网柄菌中基于激酶和 ROS 的信号传导的微电泳和统计工具

• 批准号: 2126177
• 负责人: Michelle Kovarik
• 金额: $ 29.35万
• 依托单位: Trinity College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2126177&HistoricalAwards=false
• 关键词: RUI; Microelectrophoretic; Statistical; Tools; Studies

Living organisms use a diversity of molecules to communicate information within and between cells, and scientists are still investigating the interactions between these molecules. This project will produce simultaneous measurements of both large proteins and small reactive, oxygen-containing molecules in groups of cells and in individual cells to better understand the interactions between these molecular signals. The project will also develop mathematical tools to analyze the data collected from these experiments and make these tools available to researchers doing similar work. Finally, this project will engage undergraduates and high school students in interdisciplinary research involving cell signaling, separations chemistry, microfluidics, and mathematics.The amoeba Dictyostelium discoideum undergoes a social life cycle, in which individual cells aggregate and differentiate to form a superorganism. Cell signaling during this process involves both protein kinases and reactive oxygen species, but the relationship between these interacting signaling nodes remains unclear. Through microelectrophoretic separations of peptide substrates and fluorogenic indicators, this project will simultaneously measure protein kinase B activity and superoxide concentrations in cells. In addition to characterizing these molecules in cell lysates, this project will also produce single-cell measurements using custom-designed microfluidic devices and novel mathematical strategies for data analysis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生物体使用多种分子在细胞内和细胞之间传递信息，科学家们仍在研究这些分子之间的相互作用。该项目将同时测量细胞群和单个细胞中的大蛋白质和小活性含氧分子，以更好地了解这些分子信号之间的相互作用。该项目还将开发数学工具来分析从这些实验中收集的数据，并将这些工具提供给从事类似工作的研究人员。最后，这个项目将吸引本科生和高中生参与跨学科的研究，涉及细胞信号，分离化学，微流体和数学。盘状网囊阿米巴经历了一个社会生活周期，在这个周期中，单个细胞聚集和分化形成一个超有机体。在此过程中的细胞信号转导涉及蛋白激酶和活性氧，但这些相互作用的信号节点之间的关系仍不清楚。通过肽底物和荧光指示剂的微电泳分离，该项目将同时测量细胞中蛋白激酶B活性和超氧化物浓度。除了在细胞裂解物中表征这些分子外，该项目还将使用定制设计的微流控设备和新颖的数学策略进行单细胞测量，以进行数据分析。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。