Collaborative Research: LTREB Renewal: Long-Term Dynamics of Amphibian Populations Following Disease-Driven Declines

这个项目将研究一种新的病原体入侵种群的过程，以确定一种真菌病原体导致青蛙和火蜥蜴的壶菌病。新出现的传染病的暴发日益被认为是重大威胁，不仅对人类人口，而且对野生动物也是如此，它们越来越威胁全球生物多样性。一种新的病原体最初入侵易感宿主人群可引起疾病暴发，从而导致高水平的死亡率和种群数量的下降。当这种情况发生时，宿主和病原体种群都可以发生自然选择，导致宿主对病原体感染的敏感性和耐受性以及病原体损害宿主的能力(毒力)发生进化变化。这些变化反过来又决定了宿主种群能否持续并从疾病中恢复过来。了解这些进化过程对于制定保护受威胁物种的战略至关重要。合胞菌病对世界各地的两栖动物造成了灾难性的影响，并与近几十年来许多物种的灭绝和更多的濒危物种有关。在疾病入侵加利福尼亚州内华达山脉数百个高海拔湖泊后，研究人员将调查病原体和宿主(山地黄腿青蛙)的进化变化模式。该项目将有助于理解作为自然种群进化变化动因的传染病的作用，如合孢子菌病。它将为州和联邦机构提供关键信息，促进濒危物种的恢复，并将培训和教育本科生、研究生和普通公众。这项研究基于对加利福尼亚州内华达山脉复杂景观中山地黄腿蛙(Rana sierrae和Rana Muscoa)种群动态的长期研究数据，以及树蛙入侵并通过数百个青蛙种群传播时的影响。在大多数情况下，Bd的入侵会导致该病的暴发，青蛙数量迅速下降，并导致局部灭绝。在某些情况下，青蛙种群的长期持续存在是由于Bd处于地方病状态，在这种状态下，病原体的影响大大减少。这项研究将扩展和利用25年的寄主种群和疾病数据以及来自西耶雷/金黄杆菌和Bd的存档遗传样本。这些资源将深入了解宿主和病原体种群在从病原体前到达、疾病暴发、地方性疾病到疾病前宿主种群丰度的潜在恢复过程中的变化。这个数据集将被用来研究寄主抗性/耐性和病原菌毒力差异的遗传基础。对现有青蛙和真菌样本的尖端基因组分析将补充使用Bd培养物和野生宿主群体的青蛙粘膜样本进行的Bd毒力和青蛙易感性的实验室实验。对野生宿主种群的持续调查将发现新的疾病暴发，并描述从最初的暴发到持续的地方病状态的过渡。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This project will examine the processes of invasion of a novel pathogen into a population for the case of a fungal pathogen that causes the disease chytridiomycosis in frogs and salamanders. Outbreaks of emerging infectious diseases are increasingly recognized as major threats, not just to human populations but also to wildlife, and they increasingly threaten global biodiversity. The initial invasion of a novel pathogen into a susceptible host population can cause a disease outbreak resulting in high levels of mortality and declines in population size. When this happens, natural selection can occur for both the host and pathogen populations resulting in evolutionary changes in the host's susceptibility and tolerance to infection by the pathogen and the pathogen's ability to damage the host (virulence). These changes can in turn determine whether the host population can persist and recover from the disease. Understanding these evolutionary processes is crucial in development of conservation strategies for threatened species. Chytridiomycosis has had catastrophic effects on amphibians worldwide, and has been linked to numerous species extinctions in recent decades and many more species at risk. The researchers will investigate the patterns of evolutionary change in both the pathogen and the host (mountain yellow-legged frogs), following the invasion of the disease into hundreds of high elevation lakes in the California Sierra Nevada. This project will contribute to the understanding of the role of infectious diseases, such as chytridiomycosis, as agents of evolutionary change in natural populations. It will provide critical information to state and federal agencies, facilitating endangered species recovery and will train and educate undergraduates, graduate students, and the general public.This research builds on data from a long-term study of the population dynamics of mountain yellow-legged frogs (Rana sierrae and Rana muscosa) in the complex landscape of the California Sierra Nevada, and the affects of Batrachochytrium dendrobatidis (Bd) as it has invaded and spread through hundreds of frog populations. In most cases, invasion of Bd results in outbreaks of the disease chytridiomycosis, rapid frog population declines, and local extinctions. In some cases, long-term persistence of frog populations occurs with Bd in an enzootic state in which the impact of the pathogen is greatly reduced. The research will extend and leverage 25 years of host population and disease data and archived genetic samples from R. sierrae/R. muscosa and Bd. These resources will provide insight into how populations of both host and pathogen change during the transition from pre-pathogen arrival, to disease outbreak, to enzootic disease, to potential recovery of the pre-disease host population abundances. This dataset will be used to investigate the genetic basis for differences in host resistance/tolerance and pathogen virulence. Cutting-edge genomic analysis of existing frog and fungal samples will complement laboratory experiments on Bd virulence and frog susceptibility using Bd cultures and frog mucosal samples from wild host populations. Continued surveys of wild host populations will identify new disease outbreaks and describe the transition from initial outbreak to persistent enzootic state.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.