Collaborative Research: The role of maternal iron transfer in the development of heme stores and aerobic diving capacity in grey seal pups
合作研究:母体铁转移在灰海豹幼崽血红素储备和有氧潜水能力发展中的作用
基本信息
- 批准号:2133825
- 负责人:
- 金额:$ 4.64万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2026-08-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
A fundamental question in animal ecology is when and why physiology limits how animals behave, how often they reproduce, and how likely they are to survive. Marine mammals are particularly suited for these studies because their ability to forage underwater is constrained by the size of their tissue heme and O2 stores, and pups are born with very low hemoglobin (Hb) and myoglobin (Mb) levels that can limit breathold ability. Iron is an essential micronutrient for the biosynthesis of Hb and Mb, and maternal iron status and milk iron content strongly influences early development of heme stores in many species. This project will study grey seals (Halichoerus grypus) at Sable Island, Nova Scotia to determine how maternal and offspring dietary iron dynamics impact heme maturation and subsequent foraging activities. The team will first determine maternal diet, iron stores, milk iron content and milk transfer rates. Then, researchers will provide supplemental iron to half of the study animals to determine if iron intake limits pup physiological development. Finally, the impact of heme stores on the diving behavior and movement patterns of weaned pups will be evaluated. Understanding the interplay between iron intake, physiological development and foraging behavior, may reveal how ongoing changes in grey seal diet and ecosystem structure could influence pup survival and population trajectories. This work will provide training opportunities to graduate and undergraduate students at HSI and Alaska Native serving institutions and provide outreach through a diverse suite of activities in Texas, Alaska, Canada, and Massachusetts. This study will elucidate the underlying ecological and cellular mechanisms that facilitate or constrain the ontogeny of heme and O2 stores in grey seal pups (12 pups/year/3yrs). Bulk and compound specific stable isotope analysis will be used to identify maternal diet and iron intake, while milk iron and milk transfer rates will be quantified using hydrogen isotope dynamics to determine pup iron intake. The pup’s ability to utilize milk iron will be assessed by measuring the expression of iron transporters (Divalent metal transporter 1, Ferroportin) and serum hepcidin, transferrin, and ferritin concentrations. Age-related changes in blood and muscle heme stores, iron-containing enzymes, and endocrine factors known to regulate heme protein synthesis (i.e., Erythropoietin, Hypoxia-inducible-factor-1) will be measured. Providing half the study pups with supplemental iron at the start and end of the lactation period will reveal if maternal iron transfer rates are a significant influence on pup physiological development. At the end of the post-weaning fast, pups will be outfitted with a satellite-linked dive recorder to monitor foraging patterns and locations. Behavioral comparisons between iron-supplemented and control groups will reveal whether maternal effects as mediated through micronutrient limitations influence pup foraging capacity, or if behavioral plasticity can compensate for phenotypic constraints. Studies integrating across multiple physiological and behavioral scales in juvenile marine mammals remain rare, yet understanding these linkages becomes increasingly important as climate change and human activities are causing significant shifts in the functioning of marine ecosystems across the globe.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
动物生态学中的一个基本问题是,什么时候以及为什么生理限制了动物的行为,它们繁殖的频率,以及它们存活的可能性。海洋哺乳动物特别适合于这些研究,因为它们在水下觅食的能力受到组织中血红素和氧气储存的大小的限制,而且幼崽出生时的血红蛋白(Hb)和肌红蛋白(Mb)水平非常低,这可能会限制呼吸能力。铁是Hb和Mb生物合成所必需的微量营养素,母体铁状况和乳铁含量强烈影响许多物种中血红素贮藏物的早期发育。该项目将研究新斯科舍省黑貂岛的灰海豹(Halichoerus Grypus),以确定母体和子代饮食铁的动态如何影响血红素成熟和随后的觅食活动。该研究小组将首先确定孕妇的饮食、铁储存、乳铁含量和乳汁转移率。然后,研究人员将向一半的研究动物提供补充铁,以确定铁摄入量是否限制了幼崽的生理发育。最后,将评估血红素商店对断奶幼崽的潜水行为和运动模式的影响。了解铁摄入量、生理发育和觅食行为之间的相互作用,可能会揭示灰海豹饮食和生态系统结构的持续变化如何影响幼崽的生存和种群轨迹。这项工作将为HSI和阿拉斯加土著服务机构的研究生和本科生提供培训机会,并通过在德克萨斯州、阿拉斯加、加拿大和马萨诸塞州的一系列活动提供外展服务。这项研究将阐明促进或限制灰海豹幼崽(12只/年/3岁)血红素和O2储存的个体发育的潜在生态和细胞机制。将使用大宗和化合物特定稳定同位素分析来确定母亲的饮食和铁摄入量,而乳铁和牛奶转移率将使用氢同位素动力学来量化,以确定幼崽的铁摄入量。将通过测量铁转运蛋白(二价金属转运蛋白1,铁转运蛋白)的表达和血清中海普西丁、转铁蛋白和铁蛋白的浓度来评估幼犬利用乳铁的能力。将测量血液和肌肉中的血红素储存、含铁酶和已知调节血红素蛋白质合成的内分泌因子(即促红细胞生成素、低氧诱导因子-1)随年龄的变化。在哺乳期开始和结束时,给一半的研究幼崽补充铁,将揭示母体铁转移率是否对幼崽的生理发育有重大影响。在断奶后的禁食结束时,幼崽将配备一个卫星连接的潜水记录器来监测觅食模式和位置。铁补充组和对照组之间的行为比较将揭示通过微量营养素限制介导的母体效应是否影响幼崽的觅食能力,或者行为可塑性是否可以补偿表型限制。在幼年海洋哺乳动物中整合多个生理和行为尺度的研究仍然很少见,但随着气候变化和人类活动正在导致全球海洋生态系统功能的显著变化,了解这些联系变得越来越重要。这一奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Greg Breed其他文献
Seasonal indices of nutrition and stress in a northern population of snowshoe hares
- DOI:
10.1007/s00360-025-01617-0 - 发表时间:
2025-05-07 - 期刊:
- 影响因子:1.600
- 作者:
Claire Montgomerie;Marianne Lian;Greg Breed;Mandy Keogh;Knut Kielland - 通讯作者:
Knut Kielland
Greg Breed的其他文献
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{{ truncateString('Greg Breed', 18)}}的其他基金
IntBIO Collaborative Research: An integrative approach for projecting insect responses to a rapidly changing climate
IntBIO 合作研究:预测昆虫对快速变化气候的反应的综合方法
- 批准号:
2128242 - 财政年份:2022
- 资助金额:
$ 4.64万 - 项目类别:
Standard Grant
Collaborative Research: Predator facilitation across a variable marine environment.
合作研究:在多变的海洋环境中促进捕食者。
- 批准号:
2113463 - 财政年份:2021
- 资助金额:
$ 4.64万 - 项目类别:
Standard Grant
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Cell Research
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- 批准号:10774081
- 批准年份:2007
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- 项目类别:面上项目
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