SBIR Phase I: Rapid detection of blood-borne bacteria and determination of antibiotic resistance

这个小企业创新研究（SBIR）第一阶段项目的更广泛的影响/商业潜力是加快菌血症的诊断，菌血症是细菌性败血症的根本原因，细菌性败血症是一种危及生命的疾病，其中身体对感染的反应会损伤其组织和器官。每延迟一小时给予有效的抗生素，估计死亡率增加7.6%。 该项目每年可以影响170万美国患者（全球4900万）的治疗和目前昂贵的护理。在美国，不合适的抗生素每年导致大约270，000例败血症死亡。该项目将推进抗生素敏感性的快速（1小时）鉴定，以实现从广谱抗生素治疗转向靶向引起脓毒症的特定微生物。 这个小型企业创新研究第一阶段项目探索了荧光系统在一小时内检测细菌代谢活性和确定抗生素敏感性的可行性，目标是实现高灵敏度和特异性，同时降低早期结果中常见的假阳性或阴性率。（1）设计一次性测试盒，其集成了从全血中分离细菌和引入荧光染料;（2）限制背景荧光以增加结果准确性;以及（3）该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的评估来支持。影响审查标准。

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project is to speed up the diagnosis of bacteremia, the root cause of bacterial sepsis, a life-threatening condition wherein the body’s response to an infection injures its tissues and organs. Each hour of delay in administering an effective antibiotic is associated with an estimated 7.6% increase in mortality. This project can impact the treatment and currently expensive care of 1.7 million US patients (49 million worldwide) annually. Poorly suited antibiotics contribute to roughly 270,000 sepsis deaths each year in the United States. This project will advance a rapid (1 hour) identification of antibiotic susceptibility to enable switching from broad-spectrum antibiotic treatment to targeting the specific organism causing sepsis. This Small Business Innovation Research Phase-I project explores the feasibility of a fluorescence system to detect bacterial metabolic activity and determine antibiotic susceptibility in an hour, with the goal of achieving high sensitivity and specificity while lowering false positive or negative rates common in early results This project will advance the technology in three critical areas: (1) designing a disposable test cartridge that integrates the separation of bacteria from whole blood and introduction of fluorescent dye; (2) limiting background fluorescence to increase result accuracy; and (3) developing software that provides users with easily interpreted results and reliable process control.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.