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The Aptamer BioResistor: A Broadly Applicable Protein Biosensor with Dip-and-Read Simplicity for Point-of-Care Diagnostics

Aptamer BioResistor：一种广泛适用的蛋白质生物传感器，具有简单的浸入式读取功能，可用于即时诊断

基本信息

批准号：
2149631
负责人：
Reginald Penner
金额：
$ 36万
依托单位：
University of California-Irvine
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-01-15 至 2024-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2149631&HistoricalAwards=false
关键词：
Aptamer BioResistor Broadly Applicable Protein

项目摘要

The urine test administered by an individual’s physician is not designed for the detection of any diseases. The goal of this project is to develop a simple, single-use, disposable sensor that can be dipped into a urine sample for the purpose of detecting a variety of diseases, including urinary tract infections, kidney disease, and cancers. These sensors must be inexpensive, reliable, and fast enough to test urine in a few minutes in a doctor’s office. Sensors capable of such performance are not yet available, but in previous research, a new sensor design was developed that could meet these requirements. It’s called the Impedance Transduced BioResistor or ITBR. The ITBR uses virus particles as “receptors” to do sensing. But virus particles capable of detecting disease, as required for the ITBR, are difficult to produce. The goal of this project is to adapt the ITBR so that a more common and more versatile receptor called an aptamer can be used in place of virus particles. Aptamers for some diseases are already commercially available. For other diseases, the investigators will prepare the aptamers. Aptamers are much smaller than viruses so the design of the ITBR will need to be appropriately modified. The Broader Impacts of this project include the inclusion of high school students into a summer research program in which they will learn how to make ITBRs containing aptamers.The goal of this project is to develop an Aptamer BioResistor (AptBR), in which aptamers are substituted as receptors for M13 virus particles in the Impedance-Transduced BioResistor (ITBR) developed in previous research. The Intellectual Merit of this project is in the development and characterization of an ultra thin (~100 nm) aptamer-presenting a bioaffinity layer that is compliant with the BioResistor architecture. The AptBR requires a process for the rapid and highly reproducible deposition of aptamer-conductive polymer composite films that: (1). present aptamers to a contacting solution, (2) facilitate permeation of the bioaffinity layer by target protein, and (3) electrically transduce the binding of a target protein, using the unique mechanism that operates in the ITBR. With clear design rules for the AptBR bioaffinity layer and for a new biosensor employing it, the AptBR will emerge from this project. The project has four Broader Impacts: First, the AptBR will increase the number of disease markers that can be measured using the BioResistor architecture. Second, the AptBR will be able to operate at the point-of-care to detect these diseases in animals and humans. Third, the proposal supports NEXTech 202X, an outreach program for high school students, providing hands-on laboratory science and classroom training that is keyed to the biosensor development theme of the project. Fourth, this grant will support graduate training emphasizing versatility and problem solving. These graduate students will become experienced with a broad range of tools including electron microscopy, XPS, AFM, and many others, as well as electrochemistry and microfabrication methods.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

由个人医生进行的尿检不是为检测任何疾病而设计的。该项目的目标是开发一种简单的、一次性使用的传感器，它可以浸入尿液样本中，用于检测各种疾病，包括尿路感染、肾脏疾病和癌症。这些传感器必须价格低廉、可靠、速度足够快，能够在医生的办公室里几分钟内检测出尿液。目前还没有具备这种性能的传感器，但在之前的研究中，开发了一种新的传感器设计，可以满足这些要求。它被称为阻抗转导生物电阻或ITBR。ITBR使用病毒颗粒作为“受体”进行感知。但是，像ITBR所需要的那样，能够检测疾病的病毒颗粒很难生产。这个项目的目标是调整ITBR，以便一种更常见、更通用的受体，称为适体，可以用来代替病毒颗粒。一些疾病的适体已经可以在市场上买到。对于其他疾病，研究人员将准备适体。适配体比病毒小得多，因此需要对ITBR的设计进行适当修改。该项目的更广泛影响包括将高中生纳入暑期研究计划，他们将学习如何制作含有适体的itbr。本项目的目标是开发一种适配体生物电阻（AptBR），其中适配体作为M13病毒颗粒在先前研究开发的阻抗转导生物电阻（ITBR）中的受体被取代。该项目的智力优势在于超薄（~100纳米）适配体的开发和表征-呈现符合生物电阻结构的生物亲和性层。AptBR需要一个快速和高度可复制的适配体导电聚合物复合膜沉积过程，该过程：(1)将适配体呈现在接触溶液中，(2)促进靶蛋白渗透生物亲和层，(3)利用ITBR中操作的独特机制，电转导靶蛋白的结合。有了明确的AptBR生物亲和层的设计规则和使用它的新生物传感器，AptBR将从这个项目中出现。该项目有四个更广泛的影响：首先，AptBR将增加使用生物电阻结构可以测量的疾病标志物的数量。其次，AptBR将能够在护理点检测动物和人类中的这些疾病。第三，该提案支持NEXTech 202X，这是一项针对高中生的拓展计划，提供实践实验室科学和课堂培训，这是该项目生物传感器开发主题的关键。第四，这项拨款将支持研究生培训，强调通用性和解决问题的能力。这些研究生将成为经验丰富的广泛的工具，包括电子显微镜，XPS， AFM，和许多其他的，以及电化学和微加工方法。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。