Genetically optimized M13 phages as functionalized bio-templates for the generation of bio/inorganic nanostructured materials

基因优化的 M13 噬菌体作为功能化生物模板，用于生成生物/无机纳米结构材料

• 批准号: 250530573
• 负责人: Professor Dr. Peter A. van Aken
• 金额: --
• 依托单位: Max-Planck-Institut für Festkörperforschung (MPI-FKF)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/250530573?language=en
• 关键词: Genetically; optimized; M13; phages; functionalized

Established processes for the generation of technological inorganic functional materials are subjected to restrictions in view of fabrication and material composition and properties. Miniaturized complex material assemblies with multifunctional properties are still a challenging manufacture task and the controlled adjustment of various properties is difficult. Therefore, the implementation of biomineralization routes in technical fabrication processes will circumvent such limitations. Nanometer sized, complex bio-organic structures which are formed by self-assembly processes, can be used as templates for the mineralization of inorganic materials. An excellent advantage of such templates is the adjustment to the inorganic material by genetic modification which opens the way to control material structure and properties. The main objective of this proposal is to mineralize phage-templated inorganic nanomaterials and to tune the properties of such hybrid materials by the genetic modification of M13 phage templates. Envisioned are multilayer assemblies of ZnO/gold and TiO2/gold. The following objectives shall be achieved in this project:The genetic engineering of phage templates for the specific binding and mineralization of the inorganic materials ZnO, TiO2 and goldMineralization of ZnO, TiO2, and gold on functionalized M13 templatesGeneration of organic-inorganic multilayer assemblies consisting of single materials (ZnO, TiO2, gold) and of the material combinations ZnO/gold and TiO2/goldRevealing the mineralization mechanism of the inorganics on the phage templatesEvaluation of the nanostructure of the manufactured hybrid assemblies with particular focus on the phage-inorganic interfacesDetermination of the optical, piezoelectrical, electrical, and mechanical properties of phage-templated assemblies with regard to the genetic modification of the M13 phage template.

用于产生技术无机功能材料的已建立的方法在制造和材料组成和性质方面受到限制。具有多功能特性的微型复杂材料组件仍然是一个具有挑战性的制造任务，并且各种特性的控制调节是困难的。因此，在技术制造过程中实施生物矿化途径将规避这些限制。通过自组装过程形成的纳米尺寸的复杂生物有机结构可以用作无机材料矿化的模板。这种模板的一个极好的优点是通过遗传修饰对无机材料进行调节，这开辟了控制材料结构和性能的途径。该提案的主要目标是矿化噬菌体模板无机纳米材料，并通过M13噬菌体模板的遗传修饰来调整这种混合材料的性质。设想的是ZnO/金和TiO 2/金的多层组件。本项目应实现以下目标：噬菌体模板基因工程用于无机材料ZnO、TiO 2和金的特异性结合和矿化ZnO、TiO 2和金在功能化M13模板上的矿化由单一材料组成的有机-无机多层组装体的产生（ZnO，TiO 2，金）和材料组合ZnO/金和TiO 2/金的研究揭示无机物在噬菌体模板上的矿化机制评价所制造的混合组装体的纳米结构，特别关注噬菌体-无机物界面测定光学，压电，电学，和关于M13噬菌体模板的遗传修饰的噬菌体模板化组件的机械性质。

项目成果

Genetically Induced In Situ‐Poling for Piezo‐Active Biohybrid Nanowires

• DOI: 10.1002/adma.201805597
• 发表时间: 2018-12
• 期刊: Advanced Materials
• 影响因子: 29.4
• 作者: Stefan Kilper;Timotheus Jahnke;M. Aulich;Z. Burghard;D. Rothenstein;J. Bill

Mineralization of gold nanoparticles using tailored M13 phages

• DOI: 10.1680/bbn.13.00004
• 发表时间: 2013-12
• 期刊: Bioinspired, biomimetic and nanobiomaterials
• 作者: D. Rothenstein;S. Facey;M. Ploss;P. Hans;Melanie Melcher;V. Srot;P. Aken;B. Hauer;J. Bill

Phage-assisted assembly of organic–inorganic hybrid bilayers

• DOI: 10.3139/146.111351
• 发表时间: 2016-04
• 期刊: International Journal of Materials Research
• 影响因子: 0.8
• 作者: Pouya Moghimian;Stefan Kilper;V. Srot;D. Rothenstein;S. Facey;B. Hauer;J. Bill;P. V. van Aken