SBIR Phase I: Development of a new ELISA for detection of PFAS in soil and water

SBIR 第一阶段：开发用于检测土壤和水中 PFAS 的新 ELISA

• 批准号: 2151512
• 负责人: Sejal Patel
• 金额: $ 25.58万
• 依托单位: AQUA SCIENCE LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2023-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2151512&HistoricalAwards=false
• 关键词: SBIR; Phase; Development; new; ELISA

The broader impact of this SBIR Phase I Project is to improve environmental testing. Once class of contaminants is PFAS (Per- And Polyfluoroalkyl Substances), associated with high cholesterol levels, thyroid disease, certain cancers, and pregnancy-related problems. Current testing costs $300 per sample and offers low throughput is low at 30 samples per day. The proposed project develops a test that would cost $50 per sample (6X reduction) and could test about 120 samples per day (4X increase). It could initially offer a rapid, low-cost qualitative screening tool, allowing users to determine areas of concern, hot spots, detection during emergency response and testing during mitigation efforts. The proposed project will develop a novel immunoassay in the form of enzyme-linked immunosorbent assays (ELISAs) for the detection of PFOA (perfluorooctanoic acid) and PFOS (perfluorooctane sulfonic acid), two of the most abundant PFAS in soil and water. Currently, PFAS testing involves laboratory-based, costly LC-MS (Liquid chromatography–mass spectrometry) methods. An antibody test such as an ELISA has specificity to the target compound as well as any similarly structured compounds. Thus, the test will detect multiple species of the perfluorinated compounds. During the development of PFAS ELISA, verification testing will be done in an EPA lab using LC-MS to verify the species that can be detected by the ELISA test along with the accompanying levels of detection. The research will include determination of the appropriate immunogens and haptens for this application, antibody production, and screening of antibody for specificity and sensitivity. The goal is to improve PFAS detection sensitivity to 50 ppt. Once technical feasibility of detection is demonstrated, the antibodies can be used to develop more complete assays using test strips or magnetic particles.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

SBIR第一阶段项目的更广泛影响是改善环境测试。一类污染物是PFAS（全氟烷基和多氟烷基物质），与高胆固醇水平，甲状腺疾病，某些癌症和妊娠相关问题有关。目前的测试成本为每个样品300美元，并提供低吞吐量低，每天30个样品。拟议的项目开发了一种测试，每个样本的成本为50美元（减少6倍），每天可以测试约120个样本（增加4倍）。 它最初可以提供一个快速、低成本的定性筛选工具，使用户能够确定关注领域、热点、应急反应期间的检测和缓解努力期间的测试。拟议项目将开发一种酶联免疫吸附测定法形式的新型免疫测定法，用于检测全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS），这是土壤和水中含量最多的两种全氟辛烷磺酸。目前，PFAS测试涉及基于实验室的昂贵的LC-MS（液相色谱-质谱）方法。抗体测试如ELISA对目标化合物以及任何类似结构的化合物具有特异性。因此，该测试将检测多种全氟化合物。 在PFAS ELISA开发过程中，将在EPA实验室中使用LC-MS进行验证试验，以验证ELISA试验可检出的菌种沿着伴随的检测水平。 该研究将包括确定适用于此应用的免疫原和半抗原，抗体生产以及筛选抗体的特异性和灵敏度。目标是将PFAS检测灵敏度提高到50 ppt。 一旦检测的技术可行性得到证明，抗体可用于开发更完整的检测方法，使用试纸条或磁性颗粒。该奖项反映了NSF的法定使命，并已被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估的支持。