Excellence in Research: De Novo Protein Modeling, Beyond Topology Determination Problem

卓越的研究：从头蛋白质建模，超越拓扑确定问题

• 批准号: 2153807
• 负责人: Kamal Al Nasr
• 金额: $ 53.51万
• 依托单位: Tennessee State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2153807&HistoricalAwards=false
• 关键词: Excellence; Research; De; Novo; Protein

Three-dimensional (3D) structure has a large impact on the behavior of biological systems. For example, proteins can speed up reactions, recognize and kill pathogens, or shuttle material into and out of a cell. Protein structure helps determine its ultimate function. Viruses are protected by proteins that form an outer coating, creating a viral particle. The structure of viral particles helps determine how effective a virus is in slipping past the immune system and infecting cells. Machine learning will be employed to try to understand the relationship between a protein's 3-D structure and its function. This project is expected to contribute to protein modeling and drug design. This project will also provide research and educational opportunities for students at Tennessee State University (TSU), an HBCU. This will increase the diversity of the workforce prepared to contribute to the emerging biotechnology economy.Most pharmaceutical drugs target membrane proteins. Conventional determination techniques such as X-ray crystallography and traditional computational techniques are unsuccessful with many types of proteins, such as membrane proteins, proteins that are hard to crystallize, and macromolecular proteins. Cryo-Electron Microscopy (cryo-EM) is a relatively new biophysics technique with the capability to generate volumetric images of macromolecular complexes and ensembles like ribosomes and viral capsids. Usually, cryo-EM images are large and noisy datasets. The project will investigate a novel idea to reduce the size of the image and build volume components using a geometric graph model that can drastically reduce the complexity of the problem. The volume components will be analyzed and classified to identify the secondary structure elements (SSEs) of proteins using advanced machine learning and geometric properties of the volume components. The goal of this project is to develop efficient algorithms in a fully automatic framework to identify the spatial information of the SSEs. The project has three objectives:1) Build a new mathematical model based on geometrical graph theory to reduce the size of cryo-EM images. 2) Develop efficient algorithms, including convolutional neural networks and other machine learning approaches, to process, analyze, and extract the SSEs from the volumetric images of protein macromolecule. 3) Integrate the new methods with an existing visualization and modeling platform for molecules like Chimera. If successful, this project could advance the state-of-the-art methods recently developed in protein modeling using cryo-EM by providing an automated framework that is less sensitive to noise and is segmentation-free.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

三维（3D）结构对生物系统的行为有很大的影响。例如，蛋白质可以加速反应，识别和杀死病原体，或者将物质穿梭进出细胞。蛋白质的结构决定了它的最终功能。病毒由蛋白质保护，形成一个外壳，形成一个病毒颗粒。 病毒颗粒的结构有助于确定病毒如何有效地通过免疫系统并感染细胞。机器学习将被用来试图理解蛋白质的三维结构与其功能之间的关系。预计该项目将有助于蛋白质建模和药物设计。该项目还将为田纳西州立大学（大津）的学生提供研究和教育机会。这将增加劳动力的多样性，为新兴的生物技术经济做出贡献。常规的测定技术如X射线晶体学和传统的计算技术对于许多类型的蛋白质如膜蛋白、难以结晶的蛋白质和大分子蛋白质是不成功的。冷冻电子显微镜（cryo-EM）是一种相对较新的生物物理学技术，能够生成大分子复合物和集合体（如核糖体和病毒衣壳）的体积图像。通常，冷冻EM图像是大且有噪声的数据集。该项目将研究一种新颖的想法，以减少图像的大小，并使用几何图形模型构建体积组件，可以大大降低问题的复杂性。将使用先进的机器学习和体积组分的几何特性对体积组分进行分析和分类，以识别蛋白质的二级结构元素（SSE）。这个项目的目标是在一个全自动的框架内开发有效的算法来识别SSE的空间信息。本课题的研究目标有三：1）建立一种基于几何图论的数学模型，以减小冷冻电镜图像的尺寸。2)开发有效的算法，包括卷积神经网络和其他机器学习方法，以处理，分析和提取蛋白质大分子体积图像中的SSE。3)将新方法与Chimera等分子的现有可视化和建模平台集成。如果成功的话，该项目将通过提供一个对噪声不太敏感且无分割的自动化框架，推进最近开发的使用cryo-EM进行蛋白质建模的最先进方法。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。