Collaborative Research: Regulation of exocytic membrane trafficking required for cytokinesis and polarized cell expansion

合作研究:胞质分裂和极化细胞扩张所需的胞吐膜运输的调节

基本信息

  • 批准号:
    2154572
  • 负责人:
  • 金额:
    $ 85万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-06-15 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

The plasma membrane is the outer membrane of all cells that regulates the entry and exit of material between the inside of the cell and its environment. In plants, the plasma membrane is surrounded by a cell wall that provides additional mechanical strength and protection. Production of large amounts of new plasma membrane and cell wall material is essential for plant cell division and expansion. In addition, regulation of the activity, distribution, and abundance of proteins located at the plasma membrane is highly critical for numerous cellular processes essential for growth and health of plants, including nutrient uptake, hormone signaling, pathogen perception, stress responses, and construction of the cell wall. This project will focus on understanding how the delivery of material required for plasma membrane formation and maintenance is controlled in dividing and expanding cells. Broader Impact activities include the intrinsic merit of the research as it is expected that the information gained in these studies will lead to the development of new tools and strategies for enhancing yield and quality of crop plants necessary for food security and energy independence through the improved production of biofuels and other agronomically important products. In addition to its economic and scientific benefits, this project will also provide important research training for young scientists at the undergraduate and graduate level. Students involved in this project will receive advanced training in the areas of biochemistry, genetics, and microscopy fundamental to modern cell biology research.In plants, the multisubunit SCD complex is essential for the delivery of membrane, proteins, and cell wall material to the plasma membrane by a process known as exocytosis. Major subunits of the SCD complex include the DENN domain-containing protein, SCD1 (stomatal cytokinesis defective1) and the coiled-coil protein, SCD2, which have previously been shown to be necessary for membrane trafficking required for cytokinesis and cell growth in Arabidopsis thaliana. Additionally, biochemical studies have identified another subunit of the SCD complex, MyTH1, which is related to the membrane-binding domain of metazoan class I myosins. Homologs of SCD complex subunits are present in land plants and green algae as well as other eukaryotes but were lost in yeast and metazoans, suggesting that the SCD complex is of ancient evolutionary origin. The specific aims of this project are to 1) define the biochemical activity and regulation of the SCD complex, 2) delineate the network of protein-protein and protein-lipid interactions that govern SCD complex assembly and membrane association required for exocytic membrane trafficking and 3) perform comparative studies of SCD1, SCD2, and MyTH1 homologs in the model systems Arabidopsis and Physcomitrium (Physcomitrella) patens. These combined approaches will provide insights into critical conserved and divergent functions of SCD complex subunits, necessary for exocytic trafficking, extending across the evolutionary distance between angiosperms and bryophytes. In addition, as the mechanisms of exocytosis have to date been primarily studied in yeast and metazoan model systems lacking the SCD complex, this project will generate fundamental knowledge of the exocytic molecular machinery of other plants and eukaryotes, which are important for the diversity and health of marine, freshwater, and terrestrial ecosystems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
质膜是所有细胞的外膜,它调节细胞内部和环境之间物质的进出。在植物中,质膜被细胞壁包围,细胞壁提供额外的机械强度和保护。大量新的质膜和细胞壁材料的产生是植物细胞分裂和扩增所必需的。此外,位于质膜上的蛋白质的活性、分布和丰度的调节对植物生长和健康所必需的许多细胞过程至关重要,包括营养吸收、激素信号传导、病原体感知、应激反应和细胞壁的构建。该项目将重点了解在细胞分裂和扩张过程中如何控制质膜形成和维持所需物质的输送。更广泛的影响活动包括研究的内在价值,因为预期在这些研究中获得的信息将导致开发新的工具和战略,通过改善生物燃料和其他重要农艺学产品的生产,提高粮食安全和能源独立所必需的作物产量和质量。除了经济效益和科学效益外,该项目还将为本科和研究生阶段的青年科学家提供重要的研究培训。参与该项目的学生将接受生物化学,遗传学和现代细胞生物学研究基础显微镜领域的高级培训。在植物中,多亚基SCD复合物对于细胞膜、蛋白质和细胞壁物质通过胞吐作用传递到质膜至关重要。SCD复合体的主要亚基包括含有DENN结构域的蛋白SCD1(气孔细胞分裂缺陷1)和卷曲卷曲蛋白SCD2,它们在拟南芥中被证明是细胞分裂和细胞生长所需的膜运输所必需的。此外,生化研究已经确定了SCD复合体的另一个亚基MyTH1,它与后生动物I类肌球蛋白的膜结合结构域有关。SCD复合体亚基的同源物存在于陆地植物和绿藻以及其他真核生物中,但在酵母和后生动物中丢失,这表明SCD复合体具有古老的进化起源。该项目的具体目标是:1)定义SCD复合物的生化活性和调控;2)描述控制SCD复合物组装和胞外膜运输所需的膜结合的蛋白质-蛋白质和蛋白质-脂质相互作用网络;3)在拟南芥和小立藓模型系统中对SCD1、SCD2和MyTH1同源物进行比较研究。这些结合的方法将提供对SCD复杂亚基的关键保守和不同功能的见解,这些功能是胞外运输所必需的,延伸到被子植物和苔藓植物之间的进化距离。此外,由于迄今为止,胞吐机制主要是在酵母和后生动物模型系统中研究的,缺乏SCD复合物,该项目将为其他植物和真核生物的胞吐分子机制提供基础知识,这对海洋、淡水和陆地生态系统的多样性和健康至关重要。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Sebastian Bednarek其他文献

Sebastian Bednarek的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Sebastian Bednarek', 18)}}的其他基金

Molecular analysis of SCD complex function in Rab signaling in cell morphogenesis and function
细胞形态发生和功能中 Rab 信号传导中 SCD 复合物功能的分子分析
  • 批准号:
    1614915
  • 财政年份:
    2016
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Molecular Analysis of Proteins Involved in Plant Endocytosis and Cytokinesis
参与植物内吞作用和细胞分裂的蛋白质的分子分析
  • 批准号:
    1121998
  • 财政年份:
    2011
  • 资助金额:
    $ 85万
  • 项目类别:
    Continuing Grant
Role of SCD1 in Plant Cytokinesis and Polarized Cell Expansion
SCD1 在植物细胞分裂和极化细胞扩增中的作用
  • 批准号:
    0446157
  • 财政年份:
    2005
  • 资助金额:
    $ 85万
  • 项目类别:
    Continuing Grant

相似国自然基金

Research on Quantum Field Theory without a Lagrangian Description
  • 批准号:
    24ZR1403900
  • 批准年份:
    2024
  • 资助金额:
    0.0 万元
  • 项目类别:
    省市级项目
Cell Research
  • 批准号:
    31224802
  • 批准年份:
    2012
  • 资助金额:
    24.0 万元
  • 项目类别:
    专项基金项目
Cell Research
  • 批准号:
    31024804
  • 批准年份:
    2010
  • 资助金额:
    24.0 万元
  • 项目类别:
    专项基金项目
Cell Research (细胞研究)
  • 批准号:
    30824808
  • 批准年份:
    2008
  • 资助金额:
    24.0 万元
  • 项目类别:
    专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
  • 批准号:
    10774081
  • 批准年份:
    2007
  • 资助金额:
    45.0 万元
  • 项目类别:
    面上项目

相似海外基金

Collaborative Research: Cortical Perineuronal Net Regulation of Maternal Caregiving Behaviors
合作研究:母亲护理行为的皮质神经周围网络调节
  • 批准号:
    2336907
  • 财政年份:
    2024
  • 资助金额:
    $ 85万
  • 项目类别:
    Continuing Grant
Collaborative Research: Cortical Perineuronal Net Regulation of Maternal Caregiving Behaviors
合作研究:母亲护理行为的皮质神经周围网络调节
  • 批准号:
    2336906
  • 财政年份:
    2024
  • 资助金额:
    $ 85万
  • 项目类别:
    Continuing Grant
Collaborative Research: EAR-Climate: Hydraulic and Hydrologic Regulation of Greenhouse Gas Emissions from Forest Soils and Trees and Detection With Radon As A Novel Tracer
合作研究:EAR-气候:森林土壤和树木温室气体排放的水力和水文调节以及用氡作为新型示踪剂进行检测
  • 批准号:
    2210783
  • 财政年份:
    2023
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: SCH: Psychophysiological sensing to enhance mindfulness-based interventions for self-regulation of opioid cravings
合作研究:SCH:心理生理学传感,以增强基于正念的干预措施,以自我调节阿片类药物的渴望
  • 批准号:
    2320678
  • 财政年份:
    2023
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: EAR-Climate: Hydraulic and Hydrologic Regulation of Greenhouse Gas Emissions From Forest Soils and Trees and Detection With Radon As a Novel Tracer
合作研究:EAR-气候:森林土壤和树木温室气体排放的水力和水文调节以及用氡作为新型示踪剂进行检测
  • 批准号:
    2210782
  • 财政年份:
    2023
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: EAR-Climate: Hydraulic and Hydrologic Regulation of Greenhouse Gas Emissions From Forest Soils and Trees and detection With Radon As A Novel Tracer
合作研究:EAR-气候:森林土壤和树木温室气体排放的水力和水文调节以及用氡作为新型示踪剂进行检测
  • 批准号:
    2210784
  • 财政年份:
    2023
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: Electron Heat Flux Regulation in the Solar Wind
合作研究:太阳风中的电子热通量调节
  • 批准号:
    2203319
  • 财政年份:
    2022
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: Regulation of Nuclear Size
合作研究:核尺寸的调节
  • 批准号:
    2213582
  • 财政年份:
    2022
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: PlantSynBio: Deciphering the roles of genetic and biochemical redundancy and pathway regulation via refactoring the protective plant cuticle
合作研究:PlantSynBio:通过重构保护性植物角质层破译遗传和生化冗余以及途径调节的作用
  • 批准号:
    2212800
  • 财政年份:
    2022
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
Collaborative Research: Regulation of Nuclear Size
合作研究:核尺寸的调节
  • 批准号:
    2213584
  • 财政年份:
    2022
  • 资助金额:
    $ 85万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了