Collaborative Research: Directed Enzyme Evolution Accelerated by Machine Learning for Enhancing the Biodegradation of Emerging Contaminants

合作研究：机器学习加速定向酶进化，增强新兴污染物的生物降解

• 批准号: 2203628
• 负责人: Wenwei Zheng
• 金额: $ 16.88万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203628&HistoricalAwards=false
• 关键词: Collaborative; Research; Directed; Enzyme; Evolution

The Environmental Chemical Science Program in the Division of Chemistry supports Professors Mengyan Li and Edgardo Farinas at the New Jersey Institute of Technology and Professor Wenwei Zheng at Arizona State University for this project. An increasing number of human-made chemicals are being released into the environment where they can deteriorate the water quality. Bacteria living in nature can secrete enzymes to degrade some of these chemicals and exploit them as carbon sources. However, the naturally occurring biodegradation process can be slow or inadequate to remove these contaminants. In this project, bacterial enzymes will be modified and optimized in the laboratory to speed up their degradation rates. The experimental results will be input data to train a computational model that simulates the interaction between the enzyme and the contaminant. In return, this computational model will promote the design of new enzymes with greater degradation rates. This project will initiate vigorous activities to engage graduate and undergraduate students, especially those who are members of underrepresented groups. Summer exchange workshops will be organized to promote communications between students and researchers from the New Jersey Institute of Technology and Arizona State University. Outreach efforts will be made to promote the green treatment of water contaminants and technology innovation by combining laboratory-based enzyme evaluation and computation-based machine learning.Some emerging contaminants pose imminent threats to public health and natural biota due to their frequent detection and enduring persistence in the environment. The project selects 1,4-dioxane (dioxane) as a model contaminant and employs state-of-the-art directed enzyme evaluation to gain fundamental biochemical insights into essential bacterial enzymes and ultimately optimize their biocatalytic performance for dioxane removal. Directed enzyme evolution will be used to mimic and accelerate the natural evolution in a laboratory setup, creating enzyme mutants with increased degradation efficiency towards dioxane. The rich empirical data set provided by directed enzyme evolution can be used to guide a machine learning process to predict key molecular determinants that link the protein sequence with its function and suggest new mutations for further improvement of their catalytic performance. This integrative framework will advance our fundamental knowledge regarding the biochemistry of bacterial enzymes and promote the technological transformation to combating the global challenges caused by emerging contaminants in water.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

化学系的环境化学科学项目支持新泽西理工学院的李梦妍教授和Edgardo Farinas教授以及亚利桑那州立大学的郑文伟教授进行该项目。越来越多的人造化学品被释放到环境中，它们会使水质恶化。生活在自然界中的细菌可以分泌酶来降解其中的一些化学物质，并将其作为碳源。然而，自然发生的生物降解过程可能很慢或不足以去除这些污染物。在该项目中，细菌酶将在实验室中进行修饰和优化，以加快其降解速率。实验结果将作为输入数据来训练模拟酶与污染物之间相互作用的计算模型。作为回报，这种计算模型将促进具有更高降解速率的新酶的设计。该项目将发起积极的活动，吸引研究生和本科生，特别是那些代表性不足的群体的成员。将组织夏季交流讲习班，以促进来自新泽西理工学院和亚利桑那州立大学的学生和研究人员之间的交流。通过结合实验室酶评估和基于计算的机器学习，开展外联工作，促进水污染物的绿色处理和技术创新。一些新出现的污染物因其在环境中的频繁检测和持久性而对公共健康和自然生物群构成迫在眉睫的威胁。该项目选择1，4-二氧六环（二氧六环）作为模型污染物，并采用最先进的定向酶评估，以获得对基本细菌酶的基本生物化学见解，并最终优化其生物催化性能以去除二氧六环。定向酶进化将用于在实验室设置中模拟和加速自然进化，产生对二氧六环降解效率更高的酶突变体。定向酶进化提供的丰富经验数据集可用于指导机器学习过程，以预测将蛋白质序列与其功能联系起来的关键分子决定因素，并提出新的突变以进一步改善其催化性能。这个综合框架将推进我们关于细菌酶的生物化学的基础知识，并促进技术转型，以应对水中新兴污染物造成的全球挑战。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。