Combinatorial regulation of gene expression phases

基因表达阶段的组合调控

• 批准号: 251407455
• 负责人: Professor Dr. Michael Brunner
• 金额: --
• 依托单位: Institut für Theoretische Biologie (ITB) (CCM)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/251407455?language=en
• 关键词: Combinatorial; regulation; gene; expression; phases

Eukaryotic genes are typically regulated by multiple transcription factors in a combinatorial manner. Quantitative understanding of gene regulation is particularly relevant for oscillatory expression due to transcriptional feedback loops. For periodic gene expression, the phases are essential for physiological functions.In our project we combine bioinformatic promoter analysis, large scale experiments (expression profiles and ChIP-Seq), and kinetic modeling to explore the information transfer from activators and repressors to gene expression phases. A comparative analysis of mammalian and fungal circadian rhythms allows to elucidate general design principles of phase regulation: Enhanced amplitudes via OR funnels and generation of harmonics via AND funnels.

真核基因通常以组合方式受多个转录因子调控。定量了解基因调控是特别相关的振荡表达由于转录反馈回路。在我们的项目中，我们结合联合收割机生物信息学启动子分析、大规模实验（表达谱和ChIP-Seq）和动力学模型来探索激活子和阻遏子到基因表达时相的信息传递。哺乳动物和真菌的昼夜节律的比较分析，可以阐明相位调节的一般设计原则：通过或漏斗增强振幅和通过与漏斗产生谐波。