Hierarchical organization of the network of genes controlled by the circadian clock of Neurospora

脉孢菌生物钟控制的基因网络的层次结构

• 批准号: 126258493
• 负责人: Professor Dr. Michael Brunner
• 金额: --
• 依托单位: Biochemie-Zentrum (BZH)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/126258493?language=en
• 关键词: Hierarchical; organization; network; genes; controlled

Circadian clocks coordinate expression of large networks of genes in a timeof- day specific manner and are thus crucial for the maintenance of cellular homeostasis. The White Collar Complex (WCC) is the central transcription regulator of the circadian clock of Neurospora. It may directly or indirectly modulate expression of more than 1000 clock-controlled genes. The vast majority of these genes are not known. We aim to identify WCC-activated genes by a genome-wide analysis of WCC binding sites. Using ChIP in combination with high-throughput sequencing we have identified more than 250 likely direct target genes of the WCC. Selected candidate genes are validated by various independent methods, including ChIP RT-PCR and reporter assays. Furthermore, gene products will be characterized and phenotypes of corresponding knockout strains will be analyzed. To unravel the hierarchical network of clock-controlled genes the analysis of WCCdependent genes encoding transcriptional regulators and genome wide analysis of their target genes are of particular interest.

生物钟以一天中特定的时间方式协调大型基因网络的表达，因此对维持细胞内环境稳定至关重要。白领复合体(WCC)是脉孢子虫生物钟的中心转录调节因子。它可能直接或间接调节1000多个钟控基因的表达。这些基因中的绝大多数都是未知的。我们的目标是通过对WCC结合位点的全基因组分析来识别WCC激活的基因。利用芯片结合高通量测序，我们已经确定了250多个可能的WCC直接靶基因。选择的候选基因通过各种独立的方法进行验证，包括芯片RT-PCR和报告分析。此外，还将对基因产物进行表征，并对相应的基因敲除菌株进行表型分析。为了揭开时钟控制基因的层级网络，WCC依赖的转录调控基因的分析及其靶基因的全基因组分析是特别有意义的。