PurSUiT: Systematics and species delimitation of the clownfish-hosting sea anemones.

追求：小丑鱼寄主海葵的系统学和物种界定。

• 批准号: 2205567
• 负责人: Benjamin Titus
• 金额: $ 73.9万
• 依托单位: University of Alabama Tuscaloosa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2205567&HistoricalAwards=false
• 关键词: PurSUiT; Systematics; species; delimitation; clownfish

Tropical sea anemones are important members of coral reef communities and form iconic symbiotic relationships with clownfishes and other invertebrates. However, no major efforts have been made to understand tropical sea anemone diversity since the advent of modern DNA sequencing. Compounding a lack of research, sea anemones have few physical characteristics that are useful for discerning between species and classic DNA sequencing approaches have been ineffective for discovering new taxa. Thus, it is likely that tropical sea anemone diversity is vastly underdescribed, and new genomic approaches are needed to simultaneously discover species, place them into broader evolutionary context, and determine what physical sea anemone characteristics are informative at every taxonomic level. This study will explore the evolutionary processes that have given rise to tropical sea anemone diversity by focusing on the clownfish-hosting sea anemones. The clownfish-sea anemone symbiosis has been a model for exploring fundamental biological processes, but prior research has focused almost exclusively on clownfish. The presence of undescribed sea anemone hosts will re-write our understanding of the clownfish-sea anemone symbiosis, shed light on the origin of tropical anemone diversity, and transform the interpretation of dozens of studies across scientific disciplines. This research will result in modern natural history collection resources for tropical sea anemone diversity. Outreach and educational opportunities for undergraduate students and the general public will take place at the American Museum of Natural History. Citizen science initiatives will result in the formation of the first long-term data platform to track the distribution and abundance of the clownfish sea anemones. Updated sea anemone species descriptions will be valuable for establishing scientifically-based conservation initiatives in the ornamental aquarium trade.Researchers will conduct major field research collecting expeditions to Madagascar, Western Australia, Papua New Guinea, and the Marshall Islands. All individual sea anemones sampled in this study will be photographed underwater. Resulting images and ecological metadata will be uploaded to public digital biodiversity databases. New sea anemone species will be discovered using restriction-site associated DNA sequencing and newly developed bait-capture probes for Class Anthozoa targeting Ultra Conserved Element loci. Resulting genomic data will be used for testing three key questions regarding tropical sea anemone diversity and the clownfish-sea anemone symbiosis: 1) What is the true species-level diversity of the clownfish-hosting sea anemones? 2) Do host sea anemones share a common biogeographic origin with clownfishes? 3) Are the tropics a center or origin, or accumulation, of sea anemone biodiversity? These data, which will be shared in public repositories, will provide the first comparative genomic systematic study on tropical sea anemone diversity, the clownfish-hosting sea anemones, and the anemone superfamily Actinioidea.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

热带海葵是珊瑚礁群落的重要成员，与小丑鱼和其他无脊椎动物形成标志性的共生关系。然而，自从现代 DNA 测序出现以来，并没有为了解热带海葵的多样性做出重大努力。由于缺乏研究，海葵几乎没有可用于区分物种的物理特征，而且经典的 DNA 测序方法对于发现新的分类群无效。因此，热带海葵的多样性很可能被严重低估，需要新的基因组方法来同时发现物种，将它们置于更广泛的进化背景中，并确定海葵的哪些物理特征在每个分类学层面上都提供信息。这项研究将重点关注小丑鱼寄主的海葵，探索导致热带海葵多样性的进化过程。小丑鱼与海葵的共生关系一直是探索基本生物过程的模型，但之前的研究几乎完全集中在小丑鱼上。未描述的海葵宿主的存在将改写我们对小丑鱼与海葵共生关系的理解，揭示热带海葵多样性的起源，并改变对数十项跨科学学科研究的解释。这项研究将产生热带海葵多样性的现代自然历史收藏资源。为本科生和公众提供的外展和教育机会将在美国自然历史博物馆进行。公民科学倡议将导致第一个长期数据平台的形成，以跟踪小丑鱼海葵的分布和丰度。更新的海葵物种描述对于在观赏性水族箱贸易中建立基于科学的保护举措非常有价值。研究人员将进行主要的实地研究，收集马达加斯加、西澳大利亚、巴布亚新几内亚和马绍尔群岛的探险活动。本研究中采集的所有海葵个体都将在水下拍摄。生成的图像和生态元数据将上传到公共数字生物多样性数据库。新的海葵物种将通过限制性位点相关 DNA 测序和新开发的针对超级保守元素位点的珊瑚纲诱饵捕获探针来发现。由此产生的基因组数据将用于测试有关热带海葵多样性和小丑鱼-海葵共生的三个关键问题：1）小丑鱼寄主海葵的真正物种水平多样性是什么？ 2）宿主海葵与小丑鱼有共同的生物地理起源吗？ 3) 热带地区是海葵生物多样性的中心、起源或聚集地吗？这些数据将在公共存储库中共享，将提供关于热带海葵多样性、小丑鱼宿主海葵和海葵超科海葵总科的首次比较基因组系统研究。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Clownfish hosting anemones (Anthozoa, Actiniaria) of the Red Sea: new associations and distributions, historical misidentifications, and morphological variability

• DOI: 10.1186/s41200-021-00216-6
• 发表时间: 2021-11-25
• 期刊: Marine Biodiversity Records
• 作者: Bennett-Smith, Morgan F.;Majoris, John E.;Berumen, Michael L.
• 通讯作者: Berumen, Michael L.

Comparison of sequence-capture and ddRAD approaches in resolving species and populations in hexacorallian anthozoans

序列捕获和 ddRAD 方法在解析六珊瑚动物物种和种群方面的比较

• DOI: 10.1016/j.ympev.2021.107233
• 发表时间: 2021
• 期刊: Molecular Phylogenetics and Evolution
• 影响因子: 4.1
• 作者: Glon, Heather;Quattrini, Andrea;Rodríguez, Estefanía;Titus, Benjamin M.;Daly, Marymegan
• 通讯作者: Daly, Marymegan