NSF Postdoctoral Fellowship in Biology: Investigating the Genetic and Evolutionary Dynamics Producing Patterns of Trade-off Conditionality

美国国家科学基金会生物学博士后奖学金：研究遗传和进化动力学产生的权衡条件模式

• 批准号: 2209317
• 负责人: Elisa Visher
• 金额: $ 13.8万
• 依托单位: Visher, Elisa
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2209317&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; Investigating

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2022, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment and Phenotypes. The fellowship supports research and training of the Fellow that will contribute to the area of Rules of Life in innovative ways. The Fellow’s research will focus on understanding the genetics of trade-offs in microbes to better understand patterns of diversity in nature and constraints to adaptation. The research will therefore have consequences for understanding adaptation to changing environments, anti-microbial resistance evolution, and disease emergence. The Fellow will also develop an undergraduate research program to contribute to training the next generation of researchers in biology, including those currently underrepresented in STEM disciplines.This research project will explore how trade-off shapes change over time, depend on evolutionary history, and determine coevolutionary diversification. A key goal of the project will be to understand how genetic processes determine phenotypic trade-offs, and how these processes might explain observed patterns in the conditionality of trade-offs. This will be achieved by using barcoding methods in two model systems (one evolutionary yeast system and one coevolutionary Pseudomonas and phage system) to conduct high-throughput fitness assays in multiple environments (or on coevolved partners) and track lineage dynamics. The first objective will determine how trade-off shapes change over time by further evolving existing first-step yeast mutants in specialist and generalist directions and periodically reassessing the Pareto front of evolving genotypes. The second objective will evolve yeast lineages in fluctuating environments and determine how environmental heterogeneity influences the joint distribution of mutational fitness effects. The final objective will determine how trade-off determines coevolutionary dynamics by developing methods to conduct high-throughput fitness assays and lineage tracking in Pseudomonas and phage model systems. The Fellow will gain training experience with computational and genetic methods that includes high-throughput assays and building barcoded libraries. Finally, the Fellow will contribute to broadening participation in STEM and improving undergraduate research training by developing an undergraduate research program; this will provide transparent, accessible, and supported entry into biological science research through a centralized portal advertising research opportunities and cohort-based research and professional skill training programs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动资助了2022财年的NSF生物学博士后研究奖学金，即调查基因组，环境和表型之间相互作用的生命规则的综合研究。该研究金支持研究员的研究和培训，以创新的方式为生活规则领域做出贡献。研究员的研究将侧重于了解微生物的遗传学权衡，以更好地了解自然界的多样性模式和适应的限制。因此，这项研究将对理解适应不断变化的环境，抗微生物耐药性进化和疾病的出现产生影响。该研究员还将开发一个本科生研究计划，以培养下一代生物学研究人员，包括那些目前在STEM学科代表性不足的研究人员。该研究项目将探索权衡形状如何随着时间的推移而变化，取决于进化历史，并确定共同进化多样化。该项目的一个关键目标是了解遗传过程如何决定表型权衡，以及这些过程如何解释权衡条件中观察到的模式。这将通过在两个模型系统（一个进化酵母系统和一个共同进化的假单胞菌和噬菌体系统）中使用条形码方法来实现，以在多个环境中（或在共同进化的伙伴上）进行高通量适应性测定并跟踪谱系动态。第一个目标将确定如何权衡形状随着时间的推移而变化，进一步发展现有的第一步酵母突变体的专业和通才的方向，并定期重新评估帕累托前沿的基因型发展。第二个目标是在波动的环境中进化酵母谱系，并确定环境异质性如何影响突变适应性效应的联合分布。最后的目标将确定如何权衡确定协同进化动力学的方法进行高通量健身测定和谱系跟踪假单胞菌和噬菌体模型系统。研究员将获得计算和遗传方法的培训经验，包括高通量测定和构建条形码库。最后，研究员将通过制定本科生研究计划，为扩大STEM参与和改善本科生研究培训做出贡献;这将提供透明的，可访问的，并通过一个集中的门户网站广告研究机会和队列，支持进入生物科学研究-该奖项反映了NSF的法定使命，并通过评估被认为值得支持使用基金会的知识价值和更广泛的影响审查标准。