Doctoral Dissertation Research: Phylogeographic Investigation of Aedes aegypti

博士论文研究：埃及伊蚊的系统发育地理学调查

• 批准号: 2210879
• 负责人: Crystal Hepp
• 金额: $ 3.04万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2023-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2210879&HistoricalAwards=false
• 关键词: Doctoral; Dissertation; Research; Phylogeographic; Investigation

This project investigates the geographic range and movement of the mosquito vector Aedes aegypti in arid desert environments. Arid environments are of increasing attention for mosquito control as Ae. aegypti has recently and rapidly expanded into these arid environments. Ae. aegypti mosquitoes transmit dengue, yellow fever, Zika, and chikungunya viruses. This project sequences and analyzes the entire mitochondrial genome using phylogenetic techniques and couples these data with a spatially explicit analysis of phylogenetic patterns over two seasons. This analysis contributes to an understanding of which local conditions result in source populations and high individual counts. A more complete understanding of the underlying geographic distribution and movement of Ae. aegypti in arid desert environments is beneficial to vector control and public health efforts as this mosquito continues to expand its range and outbreaks of disease occur. This understanding supports public health interventions to be targeted efficiently and effectively to those at greatest risk by establishing a “range of risk” for Ae. aegypti-borne diseases.The objective of this project is to utilize whole mitochondrial genome sequencing of mosquito pools collected over two mosquito seasons to better understand the movement of the vector within this area, as well as any potential source locations of the mosquito population. Researchers will work with public health officials to procure geo-located mosquito samples from 2019 and 2020. Data will be sequenced and analyzed to produce the entire mitochondrial genome using various phylogenetic and phylogeographic techniques. This project develops an understanding of the geographic distribution and movement of Ae. aegypti and identifies potential source locations for the mosquito population in arid environments.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目调查了蚊子媒介埃及伊蚊在干旱沙漠环境中的地理范围和移动。干旱环境是蚊虫防治的重点。埃及最近迅速扩展到这些干旱环境。AE.埃及蚊子传播登革热、黄热病、寨卡和基孔肯雅病毒。该项目使用系统发育技术对整个线粒体基因组进行测序和分析，并将这些数据与两个季节的系统发育模式的空间显式分析相结合。这种分析有助于了解哪些当地条件导致源种群和高个体计数。更全面地了解Ae的潜在地理分布和运动。干旱沙漠环境中的埃及伊蚊对病媒控制和公共卫生工作是有益的，因为这种蚊子继续扩大其活动范围，并发生疾病暴发。这种理解支持公共卫生干预措施，以有效率和有效地针对那些在最大的风险，建立一个“风险范围”的Ae。该项目的目标是利用两个蚊子季节收集的蚊子池的全线粒体基因组测序，以更好地了解该地区内病媒的移动以及蚊子种群的任何潜在来源地点。研究人员将与公共卫生官员合作，从2019年和2020年获得地理定位的蚊子样本。将对数据进行测序和分析，以使用各种系统发育和地理学技术产生整个线粒体基因组。该项目旨在了解Ae的地理分布和运动。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。