B cellular regulation of allergen tolerance during specific immunotherapy of IgE-mediated respiratory allergies

在本项目中，将解决以下假设：i)在特异性免疫治疗(SIT)过程中，过敏原特异性B细胞通过产生过敏原阻断抗体(分泌调节性细胞因子)并在鼻黏膜中表现出更高的调节活性来作为过敏原耐受性的介体。ii)不同的过敏原应用模式(皮下和舌下SIT)导致明显不同的免疫变化。iii)长期过敏原耐受性取决于过敏原特异性记忆B细胞反应的诱导，该反应可以通过过敏原增强剂注射来刺激。

In this project the following hypotheses will be addressed:I) During the course of specific immunotherapy (SIT), allergen-specific B cells serve as mediators of allergen tolerance by - producing allergen-blocking antibodies- secreting regulatory cytokines- and showing increased regulatory activity in the nasal mucosa.II) Different modes of allergen application (subcutaneous vs. sublingual SIT) result in distinctly different immune alterations.III) Long term allergen tolerance depends on the induction of an allergen-specific memory B cell response, which can be stimulated by allergen booster injections.