LEAPS-MPS: Peroxisome Targeting Chemical Technologies and Tools to Study Subcellular Chemistry

LEAPS-MPS：过氧化物酶体靶向化学技术和工具来研究亚细胞化学

• 批准号: 2213273
• 负责人: Michael Beck
• 金额: $ 25万
• 依托单位: Eastern Illinois University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2213273&HistoricalAwards=false
• 关键词: LEAPS; MPS; Peroxisome; Targeting; Chemical

This award is funded in whole or in part under the American Rescue Plan Act of 2021 (Public Law 117-2). In this project, Professor Beck and his students at Eastern Illinois University (EIU) intend to develop chemical groups that can direct attached molecules to the peroxisomes, an understudied region of cells that has an important role in a variety of cellular processes. The new chemical approaches and tools developed in this project have the potential to enable detailed study of the biochemical reactions that take place in the peroxisomes of living cells. Successful implementation of this project will also broaden participation of students underrepresented in STEM fields by providing paid research opportunities for students and strengthening the Inclusiveness in STEM (iSTEM) program at EIU. The iSTEM program is designed to provide these students more opportunities to interact with potential role models and mentors.Prof. Beck and his team intend to adapt peroxisome targeting peptide sequences to develop modular moieties that can target pendant small molecule cargoes to the peroxisomes. Successful implementation of this research will result in the characterization peroxisome interaction moieties (PIMs). Properties include cell permeability, interaction of key components of the peroxisome import mechanism, and the ability to direct previously untargeted compounds to the peroxisomes. PIMs will then be used to generate fluorogenic probes to monitor hydrogen peroxide production in the peroxisomes. An expected outcome of the project is to assist students in developing the connections and skills they need to be successful in their future studies and/or careers in STEM while making significant scientific contributions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项的全部或部分资金来自《2021年美国救援计划法案》(公法117-2)。在这个项目中，Beck教授和他在东伊利诺伊大学(EIU)的学生打算开发能够将分子直接连接到过氧化体的化学基团，过氧化体是一个未被研究的细胞区域，在各种细胞过程中发挥着重要作用。在这个项目中开发的新的化学方法和工具有可能使详细研究在活细胞的过氧化物体中发生的生化反应成为可能。该项目的成功实施还将通过为学生提供有偿研究机会并加强经济学人智库STEM(ISTEM)计划的包容性，扩大STEM领域代表性不足的学生的参与。ISTEM项目旨在为这些学生提供更多与潜在榜样和导师互动的机会。Beck和他的团队打算修改Peroxisome靶向多肽序列，以开发能够靶向Peroxisome的悬挂小分子货物的模块部分。这项研究的成功实施将导致对过氧化体相互作用部分(PIM)的表征。性质包括细胞通透性，过氧化体输入机制的关键成分的相互作用，以及将先前未靶向的化合物定向到过氧酶体的能力。然后，PIM将被用来产生荧光探针，以监测过氧化物体中过氧化氢的产生。该项目的一个预期结果是帮助学生发展他们在未来STEM学习和/或职业生涯中取得成功所需的联系和技能，同时做出重大的科学贡献。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。