MRI: Acquisition of a SEC-MALS Instrument for Research and Teaching at California State University, Sacramento

该奖项由主要研究仪器和化学研究仪器计划共同支持。加州州立大学萨克拉门托分校（Sac State）正在收购一种用于多角度光散射分子排阻色谱（SEC-MALS）分析的仪器，该仪器将包括一台配有UV检测器和自动进样器的HPLC仪器，用于高通量分析，一台折射率（RI）检测器和一台MALS检测器，以支持凯瑟琳麦克雷诺兹教授同事琳达罗伯茨和斯特凡保拉的研究。简言之，SEC-MALS是用于测定溶液中分子的摩尔质量和尺寸的绝对技术。该仪器将尺寸排阻色谱与多角度光散射相结合，提供了先进的表征技术，并克服了柱校准的许多局限性。SEC-MALS是一种非常有价值和有用的技术，用于表征各种大分子，包括合成聚合物，脂质，蛋白质和其他生物材料。此次收购加强了大学和地区的研究基础设施。该工具增强了许多部门各级学生的教育，研究和教学努力，并提供了在附近机构使用的无障碍环境。该工具将用于培训主要本科院校的学生群体，这些学生群体也被归类为西班牙裔服务和亚裔美国人/美洲土著人/太平洋岛民服务，其中很大一部分学生群体被确定为代表性不足的少数群体。该仪器为学生提供了使用重要仪器的经验，他们随身携带到他们的职业生涯。该仪器包括多角度光散射尺寸排阻色谱（SEC-MALS），旨在加强各级研究和教育。它特别影响包括化学，生物化学和聚合物科学在内的领域。研究重点是合成/生物大分子的分析，并获得有关这些分子的重要结构信息。这项工作包括研究合成的糖共聚物和各种病毒刺突蛋白（HIV和SARS-CoV-2）之间的相互作用，以更好地了解早期病毒与宿主细胞的结合。该仪器还将使脂质体作为益生元生命形式的模型的研究成为可能，使用该仪器来确定脂质体制剂的直径和稠度，以及检查apoA-I的异常寡聚化，该奖项反映了NSF的法定使命，并通过使用基金会的智力价值进行评估，被认为值得支持和更广泛的影响审查标准。

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. California State University-Sacramento (Sac State), is acquiring an instrument for size exclusion chromatography with multi- angle light scattering (SEC-MALS) analysis, which will include an HPLC instrument with a UV detector and an autosampler for high throughput analysis, a refractive index (RI) detector, and a MALS detector to support the research of Professor Katherine McReynolds colleagues Linda Roberts and Stefan Paula. In brief, SEC-MALS is an absolute technique for determining molar mass and size of molecules in solution. The instrument involves the combination of size-exclusion chromatography with multi-angle light scattering and offers an advanced characterization technique and overcomes the many limitations of column calibration. SEC-MALS is an extremely valuable and useful technique for the characterization of a wide variety of macromolecules, including synthetic polymers, lipids, proteins, and other biomaterials. The acquisition strengthens the research infrastructure at the University and regional area. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. The instrument will be used to trains a student population at the primarily undergraduate institution that is also classified as Hispanic serving and Asian American/Native American/Pacific Islander serving with a large portion of the student population identifying as being from underrepresented minority groups. The instrument gives students experience using vital instrumentation that they carry with them into their careers.The award of this instrument that includes size exclusion chromatography with multi- angle light scattering (SEC-MALS) is aimed at enhancing research and education at all levels. It especially impacts areas including chemistry, biochemistry and polymer science. Research is focused on the analysis of synthetic/biologic macromolecules and derive important structural information about these molecules. This work includes the study of the interactions between synthetic glycopolymers and various viral spike proteins (HIV and SARS-CoV-2) to better understand early-stage viral binding to host cells. The instrument will also enable the study of liposomes as models for prebiotic life forms, using the instrument to determine diameter and consistency of liposome preparations, as well as the examination of the abnormal oligomerization of apoA-I, which contributes to deposition of amyloid protein in arterial plaque.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.