RUI: Molecular Mechanisms of Short-Range Electron Transfer in Metalloproteins

RUI:金属蛋白短程电子转移的分子机制

基本信息

  • 批准号:
    2216956
  • 负责人:
  • 金额:
    $ 73.79万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-08-01 至 2027-07-31
  • 项目状态:
    未结题

项目摘要

This project is focused on understanding the molecular level mechanism of short-range electron transfer in proteins. All living systems obtain energy through electrons occupying high energy states, either through respiratory chains (food) or from light (photosynthesis). Electron transfer is a vital function of the many proteins responsible for storage, transfer, and transformation of this energy. While there is some understanding of the factors controlling charge transfer over longer distances, little is known about biological electron transfer at distances shorter than 1 nm. Filling this gap will advance our knowledge of the fundamental steps in bioenergetics. By developing computational and experimental models to predict and explain the key parameters of these reactions, a cohesive understanding of short-range biological electron transfer will be obtained. In addition, the project will provide research training for undergraduates and underrepresented minority students and will also support innovations in the science curriculum. This project will also facilitate curricular updates in several upper-level laboratory courses at James Madison University. PpcA, a 3 heme c-type cytochrome from Geobacter sulfurreducens, genetically modified and covalently labeled with several photosensitizers will be used as a model system to study charge transfer reactions. The overarching hypothesis is that tight structural coupling and effective dissipation of excess heat energy are essential to achieve ultrafast charge transfer rates. The kinetics of the reactions will be studied with time-resolved fluorescence and absorbance spectroscopies at room and cryogenic temperatures. The structural integrity of protein-photosensitizer complexes will be monitored with SAXS and CD spectroscopy. Extensive all-atom molecular dynamics simulations will be performed to predict structures and to evaluate structural dynamics. These predictions will be tested experimentally with several structural techniques including nuclear magnetic resonance spectroscopy and x-ray crystallography. The collected kinetic data will be analyzed in the context of molecular structures and will be used to test and revise currently available computational and theoretical approaches for the prediction of electron transfer rates and pathways. This project is funded by the Molecular Biophysics Cluster in the Division of Molecular and Cellular Biosciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目的重点是了解蛋白质中短程电子转移的分子水平机制。所有的生命系统都通过占据高能态的电子获得能量,或者通过呼吸链(食物),或者从光(光合作用)。电子转移是许多蛋白质的重要功能,负责储存,转移和转化这种能量。虽然有一些了解的因素控制电荷转移在较长的距离,鲜为人知的是生物电子转移的距离小于1纳米。填补这一空白将推进我们对生物能量学基本步骤的认识。通过开发计算和实验模型来预测和解释这些反应的关键参数,将获得对短程生物电子转移的有凝聚力的理解。此外,该项目还将为本科生和代表性不足的少数民族学生提供研究培训,并将支持科学课程的创新。该项目还将促进詹姆斯麦迪逊大学几门高级实验室课程的课程更新。 PpcA,一个3血红素C型细胞色素从Geetriumsulfurreducens,基因修饰和共价标记的几种光敏剂将被用作模型系统来研究电荷转移反应。首要的假设是,紧密的结构耦合和有效的耗散多余的热能是必不可少的,以实现超快的电荷转移速率。反应的动力学将在室温和低温下用时间分辨荧光和吸收光谱进行研究。将用SAXS和CD光谱法监测蛋白质-光敏剂复合物的结构完整性。将进行广泛的全原子分子动力学模拟来预测结构和评估结构动力学。这些预测将通过几种结构技术进行实验测试,包括核磁共振光谱学和X射线晶体学。收集的动力学数据将在分子结构的背景下进行分析,并将用于测试和修订目前可用的计算和理论方法,用于预测电子转移速率和途径。该项目由分子和细胞生物科学部的分子生物物理学小组资助。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Oleksandr Kokhan其他文献

Experimental and Computational Studies of Obscurin's Flexibility
  • DOI:
    10.1016/j.bpj.2017.11.2260
  • 发表时间:
    2018-02-02
  • 期刊:
  • 影响因子:
  • 作者:
    Jake Whitley;Daniel Marzolf;Oleksandr Kokhan;Nathan Wright
  • 通讯作者:
    Nathan Wright
Recrystallization and heterovalent substitution effects on mechanical and electrical parameters of Agsub6+x/sub(Psub1−x/subGesubx/sub)Ssub5/subI–based ceramics
再结晶和异价取代对Ag₆₊ₓ(P₁₋ₓGeₓ)S₅I基陶瓷力学和电学参数的影响
  • DOI:
    10.1016/j.jeurceramsoc.2023.12.093
  • 发表时间:
    2024-06-01
  • 期刊:
  • 影响因子:
    6.200
  • 作者:
    Artem Pogodin;Mykhailo Filep;Tetyana Malakhovska;Vasyl Vakulchak;Vladimir Komanicky;Serhii Vorobiov;Vitalii Izai;Leonid Satrapinskyy;Iryna Shender;Vitaliy Bilanych;Oleksandr Kokhan;Peter Kúš
  • 通讯作者:
    Peter Kúš
Engineering a Cytochrome with a Tunable Bandgap Potential
  • DOI:
    10.1016/j.bpj.2018.11.3118
  • 发表时间:
    2019-02-15
  • 期刊:
  • 影响因子:
  • 作者:
    Samuel D. Fontaine;Coleman Swaim;P. Raj Pokkuluri;Oleksandr Kokhan
  • 通讯作者:
    Oleksandr Kokhan
Control Over Protein Multimerization Induced by Water-Soluble Porphyrins
  • DOI:
    10.1016/j.bpj.2020.11.1383
  • 发表时间:
    2021-02-12
  • 期刊:
  • 影响因子:
  • 作者:
    Tyler Brittain;Oleksandr Kokhan
  • 通讯作者:
    Oleksandr Kokhan
Long-Range Regulation of Cytochrome <em>C</em> Bindingto <em>Bc<sub>1</sub></em> Complex
  • DOI:
    10.1016/j.bpj.2018.11.2248
  • 发表时间:
    2019-02-15
  • 期刊:
  • 影响因子:
  • 作者:
    Spencer B. Grewe;Oleksandr Kokhan
  • 通讯作者:
    Oleksandr Kokhan

Oleksandr Kokhan的其他文献

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{{ truncateString('Oleksandr Kokhan', 18)}}的其他基金

RUI: Molecular Mechanisms of Short-Range Electron Transfer in Metalloproteins
RUI:金属蛋白短程电子转移的分子机制
  • 批准号:
    1817448
  • 财政年份:
    2018
  • 资助金额:
    $ 73.79万
  • 项目类别:
    Standard Grant

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    面上项目
Molecular Plant
  • 批准号:
    31024802
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    2010
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    20.0 万元
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    30824806
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    2008
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  • 项目类别:
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RUI: Collaborative Research: Molecular mechanisms of dendrite development, maintenance and plasticity: in vivo single-neuron analysis in C. elegans
RUI:合作研究:树突发育、维持和可塑性的分子机制:线虫体内单神经元分析
  • 批准号:
    1754986
  • 财政年份:
    2019
  • 资助金额:
    $ 73.79万
  • 项目类别:
    Continuing Grant
RUI: Molecular Mechanisms of Short-Range Electron Transfer in Metalloproteins
RUI:金属蛋白短程电子转移的分子机制
  • 批准号:
    1817448
  • 财政年份:
    2018
  • 资助金额:
    $ 73.79万
  • 项目类别:
    Standard Grant
RUI: Investigating the Molecular Mechanisms of Non-muscle Myosin II Contractility
RUI:研究非肌肉肌球蛋白 II 收缩性的分子机制
  • 批准号:
    1716964
  • 财政年份:
    2017
  • 资助金额:
    $ 73.79万
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    Standard Grant
RUI: Sulfur Chemistry: Molecular Mechanisms
RUI:硫化学:分子机制
  • 批准号:
    1566282
  • 财政年份:
    2016
  • 资助金额:
    $ 73.79万
  • 项目类别:
    Standard Grant
RUI: Characterizing Protein Homeostasis and the Regulatory Mechanisms Controlling Molecular Chaperone Expression in the Highly Stenothermal Notothenioid Fish, Trematomus Bernacchii
RUI:表征蛋白质稳态和控制分子伴侣表达的调节机制在高度窄温的诺托尼鱼,Trematomus Bernacchii
  • 批准号:
    1543419
  • 财政年份:
    2016
  • 资助金额:
    $ 73.79万
  • 项目类别:
    Standard Grant
RUI (MCB - Genetic Mechanisms): Molecular analysis of two interacting components of the conjugation machinery of Bacillus subtilis
RUI(MCB - 遗传机制):枯草芽孢杆菌接合机制的两个相互作用成分的分子分析
  • 批准号:
    1613920
  • 财政年份:
    2016
  • 资助金额:
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RUI: The Function and Molecular Mechanisms of Novel RNAs in E. Coli
RUI:大肠杆菌中新型 RNA 的功能和分子机制
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Collaborative Research: RUI: Molecular mechanisms and physiological triggers underlying neuromodulator plasticity in a lobster pattern generator
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  • 批准号:
    1353023
  • 财政年份:
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    $ 73.79万
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Collaborative Research: RUI: Molecular mechanisms and physiological triggers underlying neuromodulator plasticity in a lobster pattern generator
合作研究:RUI:龙虾模式发生器中神经调节剂可塑性的分子机制和生理触发因素
  • 批准号:
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    Standard Grant
RUI: Molecular Mechanisms of Flower Reversion in Arabidopsis suecica
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    1118539
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