Collaborative Research: Protein engineering and processing of plant viral templates for controlled nanoparticle synthesis

合作研究：用于受控纳米颗粒合成的植物病毒模板的蛋白质工程和加工

• 批准号: 2219276
• 负责人: Kevin Solomon
• 金额: $ 62.16万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2219276&HistoricalAwards=false
• 关键词: Collaborative; Research; Protein; engineering; processing

Nano-scaled materials have unique properties that are tied to their size. The properties of these materials are controlled by their architecture. As a result, their manufacture must be tightly controlled. A uniform template would be extremely useful in such processes. Barley stripe mosaic virus (BSMV) is a plant virus with highly uniform structure. Using templates derived from BSMV is proposed as an economical alternative to current processes. Virus-like particles (VLPs) derived from BSMV will be designed to act as templates for the production of metallic nanomaterials. Graduate and undergraduate students in will be trained to design and produce these templates. High school students from underrepresented groups and the general public will be engaged through innovative workshops that teach them about the power of biological engineering and nanomaterials. BSMV is an attractive biotemplate for synthesis of metallic nanomaterials. It can be more densely coated with metal than other biotemplates. It interacts electrostatically with metal precursor ion solutions to deposit a wider range of metals. It requires fewer processing steps to coat fully. The role of key BSMV amino acid residues in mediating metal mineralization on the VLP surface will be investigated. Protein and RNA engineering strategies to control biotemplate properties and facilitate biotemplate robustness will be pursued. Surface residues will be altered to facilitate metal-template interactions. Thermal processing to create biotemplated-materials with high electrical conductivities will be optimized. If successful, the techniques developed will create an economical bacterial expression and purification pipeline for designer BSMV biotemplates. The range of metals that ca be deposited on them will be broadened. Key insights into organic-inorganic interactions involved in metal deposition on biotemplates will be uncovered. The project results may elucidate structural details about how BSMV capsid proteins self-assemble, having implications for the infection and treatment of cereal crops that are susceptible to BSMV.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纳米级材料具有与其尺寸相关的独特特性。这些材料的特性由其结构控制。因此，它们的制造必须受到严格控制。统一的模板在此类过程中将非常有用。大麦条纹花叶病毒（BSMV）是一种结构高度均一的植物病毒。建议使用源自 BSMV 的模板作为当前流程的经济替代方案。源自 BSMV 的病毒样颗粒（VLP）将被设​​计用作生产金属纳米材料的模板。 研究生和本科生将接受设计和制作这些模板的培训。来自弱势群体和公众的高中生将参与创新研讨会，向他们传授生物工程和纳米材料的力量。 BSMV 是一种用于合成金属纳米材料的有吸引力的生物模板。 与其他生物模板相比，它可以更致密地涂覆金属。它与金属前体离子溶液发生静电相互作用，沉积更广泛的金属。完全涂覆所需的加工步骤较少。将研究关键 BSMV 氨基酸残基在介导 VLP 表面金属矿化中的作用。将寻求控制生物模板特性和促进生物模板稳健性的蛋白质和RNA工程策略。表面残基将被改变以促进金属-模板相互作用。用于制造具有高电导率的生物模板材料的热处理将得到优化。如果成功，所开发的技术将为设计 BSMV 生物模板创建经济的细菌表达和纯化管道。可沉积在其上的金属范围将会扩大。将揭示生物模板上金属沉积所涉及的有机-无机相互作用的关键见解。该项目结果可能阐明 BSMV 衣壳蛋白如何自组装的结构细节，对易受 BSMV 影响的谷类作物的感染和治疗具有影响。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Engineering alkaline-stable barley stripe mosaic virus-like particles for efficient surface modification

• DOI: 10.1016/j.bej.2023.109062
• 发表时间: 2023-08-21
• 期刊: BIOCHEMICAL ENGINEERING JOURNAL
• 影响因子: 3.9
• 作者: Vaidya，Akash J.;Rammohan，Mruthula;V. Solomon，Kevin
• 通讯作者: V. Solomon，Kevin