I-Corps: Commercialization of a real-time, palm-size, low-cost, and wireless SARS-COV-II detector

I-Corps项目更广泛的影响/商业潜力是开发一种无线纳米生物传感器，用于检测活的冠状病毒颗粒和潜在的其他病原体。目前，基于酶联免疫吸附试验(ELISA)或聚合酶链式反应(PCR)的COVID检测的阳性结果并不能确定冠状病毒颗粒是活的(有感染性的)还是死亡的(或中和的或非传染性的)。这项拟议的技术旨在实时检测活冠状病毒颗粒与目标细胞、抗体和药物的相互作用。那些被提议的技术结果为阴性的人已经确认，他们没有活的、有传染性的冠状病毒颗粒，不需要隔离。这项技术可能为有效抗击新冠肺炎病毒和未来的大流行/流行病以及其他感染提供一种工具。这个i-Corps项目基于最初专注于SARS COV-II的病原体检测的纳米生物传感方法的开发。该技术将生物陶瓷纳米线/石墨烯核/壳纳米复合材料与无线通信芯片表面的射频识别(RFID)标签集成在一起。纳米生物传感系统的硬件由射频纳米传感器、射频阅读器、手持设备或智能手机三部分组成。所提出的纳米生物传感器是通过将生物安全纳米复合材料涂覆在一次性RFID标签上来制造的，以最大限度地提高灵敏度。此外，可以集成纳米生物传感器来同时检测多孔板中的样本，该多孔板在检测病毒颗粒、病毒抗原和抗体以及评估病毒中和抗体和疫苗效力方面是通用和有效的。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this I-Corps project is the development of a wireless nano-biosensor for detection of live coronavirus particles and potentially other pathogens. Currently, a positive result from an enzyme-linked immunosorbent assay (ELISA)- or polymerase chain reaction (PCR)-based COVID test does not determine if the coronavirus particles are live (infectious) or dead (or neutralized or noninfectious). The proposed technology is designed to detect interactions of live coronavirus particles with target cells, antibodies, and drugs in real-time. Those with a negative result from the proposed technology have confirmation that they are free of live, infectious coronavirus particles and would not need to quarantine. This technology may provide a tool for effectively combatting the COVID-19 virus and future pandemics/epidemics as well as other infections.This I-Corps project is based on the development of a nano-biosensing methodology for pathogen detection initially focused on SARS COV-II. The proposed technology integrates a bio-ceramic nanowire/graphene core/shell nanocomposite with a radio frequency identification (RFID) tag on a wireless communication chip surface. The hardware of the nano-biosensing system is composed of three parts: a radio frequency nano-sensor, a radio frequency reader, and a handheld device or smart phone. The proposed nano-biosensor is made by coating the biosafe nanocomposite on a disposable RFID tag to maximize the sensitivity. Further, nano-biosensors may be integrated to simultaneously detect samples in a multi-well plate, which is versatile and effective in the detection of virus particles, viral antigens, and antibodies, as well as in evaluating virus-neutralizing antibodies and vaccine efficacy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.