Collaborative Research: RUI: Extraordinary circadian clocks in araneoid spiders: an integrative approach to understanding their evolutionary origins and underlying mechanisms

合作研究:RUI:类蜘蛛的非凡生物钟:一种理解其进化起源和潜在机制的综合方法

基本信息

  • 批准号:
    2235712
  • 负责人:
  • 金额:
    $ 31.81万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-06-01 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

Circadian rhythms are daily rhythms of behavior, physiology, and cellular metabolism that are driven by molecular cycles of an internal biological clock. Internal clocks remain in sync with the earth’s 24-h day by making small adjustments to their own internal clock period. However, there are negative physiological consequences if the clock is forced to make large adjustments (e.g., jetlag) so most organisms’ clocks do not vary much from 24 hours. Surprisingly, a group of spider species possess clocks that differ from 24 hours by as much as 5 hours with no apparent consequences. In theory, these species should not exist! However, the spider system provides a unique opportunity to explore basic mechanisms of circadian clocks, particularly how organisms synchronize with their environment. This multi-institutional project is designed to understand: (1) the evolutionary changes in clock genes and circadian properties in spiders, (2) the limits and physiological consequences of synchronizing to the 24-h day, and (3) the fundamental molecular clockworks of spiders. Overall, this project will develop a new, and uniquely powerful, model system to understand circadian rhythms and, potentially, circadian illnesses. Circadian rhythms are conceptually accessible to students and this project will support rich opportunities for undergraduates in the Appalachian region to participate in research at all three institutions. Societal impacts of this project will include development of user-friendly, open-access applications for rigorous analyses of circadian data, annual public outreach events including nature/STEM programs for K-12 students as well as adults, and conducting original experiments in local high schools. Araneoid spider circadian rhythms are unlike most others found on Earth. They exhibit remarkably broad distributions of endogenous free-running periods (FRPs) both within and among species, including species with exceptionally short or long mean FRPs (17.8-29.1 hours). Rather than suffering negative consequences typically associated with dissonance between endogenous circadian period and the 24-hour day, survivorship experiments suggest that they are somehow released from these selective constraints. Using an integrative, multi-level approach, this project will exploit the apparent evolutionary shift in circadian clock system function between araneoid and non-araneoid spider species to identify changes in the clock mechanisms that enable these unusual araneoid clocks to exist. Using genomic or transcriptomic-scale data to estimate rates of evolutionary change in eight different chronobiological parameters for species spanning the diversity of spiders will enable reconstruction of ancestral states and pinpoint the timing of evolutionary shifts. Comparing survivorship among araneoid and non-araneoid species will determine if araneoid spiders truly have been released from the costs of entrainment to non-resonant light/dark cycles. Classic phase-shifting and phase-response curve experiments will probe differences in entrainment potential between araneoid and non-araneoid spiders. Comparing canonical clock gene expression amplitudes between araneoid and non-araneoid spiders and to established insect models will explore potential clock function differences. Comparing the functionality of the clock component CRY1 between araneoid and non-araneoid species will test for differences in light sensitivity of the circadian system. Using molecular, behavioral, and phylogenetic approaches, this project explores the extent and functional ramifications of circadian plasticity in wild clocks.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
昼夜节律是由内部生物钟的分子周期驱动的行为、生理和细胞代谢的每日节律。内部时钟通过对自己的内部时钟周期进行微小的调整来与地球的24小时保持同步。然而,如果时钟被迫进行大的调整(例如,时差),所以大多数生物体的生物钟与24小时相差不大。 令人惊讶的是,一组蜘蛛物种的生物钟与24小时相差多达5小时,而没有明显的后果。从理论上讲,这些物种不应该存在。然而,蜘蛛系统提供了一个独特的机会来探索生物钟的基本机制,特别是生物体如何与环境同步。这个多机构项目旨在了解:(1)蜘蛛生物钟基因和昼夜节律特性的进化变化,(2)同步到24小时的限制和生理后果,以及(3)蜘蛛的基本分子时钟。总的来说,该项目将开发一个新的,独特的强大的模型系统,以了解昼夜节律和潜在的昼夜疾病。昼夜节律是概念上接近学生,这个项目将支持丰富的机会,在阿巴拉契亚地区的本科生参加在所有三个机构的研究。该项目的社会影响将包括开发用户友好的开放式应用程序,用于严格分析昼夜节律数据,年度公共宣传活动,包括K-12学生和成人的自然/STEM项目,以及在当地高中进行原创实验。类圆蛛的昼夜节律与地球上的大多数蜘蛛不同。它们在物种内部和物种之间表现出非常广泛的内源性自由运行期(FRPs)分布,包括具有异常短或长的平均FRPs(17.8-29.1小时)的物种。而不是遭受负面后果通常与内源性昼夜节律周期和24小时之间的不和谐,生存实验表明,他们以某种方式从这些选择性约束中释放出来。使用综合的,多层次的方法,该项目将利用蜘蛛和非蜘蛛物种之间的生物钟系统功能的明显进化转变,以确定使这些不寻常的蜘蛛时钟存在的时钟机制的变化。使用基因组或转录组学规模的数据来估计跨越蜘蛛多样性的物种的八种不同时间生物学参数的进化变化率,将能够重建祖先状态并确定进化转变的时间。比较蜘蛛类和非蜘蛛类物种之间的生存率将确定蜘蛛类蜘蛛是否真的已经从非共振光/暗周期的夹带成本中释放出来。经典的相移和相位响应曲线实验将探讨蜘蛛和非蜘蛛之间的夹带潜力的差异。比较类蜘蛛和非类蜘蛛之间的典型时钟基因表达幅度,并建立昆虫模型,将探讨潜在的时钟功能差异。比较类蜘蛛和非类蜘蛛物种之间时钟组件的功能将测试昼夜节律系统的光敏感性的差异。利用分子,行为和系统发育的方法,该项目探讨了野生clocks.This奖项反映了NSF的法定使命的昼夜节律可塑性的程度和功能的后果,并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

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Jessica Petko其他文献

Determining the photic chronotype in locomotor activity and levels of endogenous control in the American dog tick em(Dermacentor variabilis)/em
确定美洲犬蜱(Dermacentor variabilis)运动活动中的光时型和内源性控制水平
  • DOI:
    10.1016/j.ttbdis.2025.102461
  • 发表时间:
    2025-03-01
  • 期刊:
  • 影响因子:
    3.400
  • 作者:
    Joseph Giulian;Natalia Toporikova;Jessica Petko;Nadia Ayoub;Thomas C. Jones;Darrell Moore
  • 通讯作者:
    Darrell Moore
Alternative splicing of the Wnt trafficking protein, Wntless and its effects on protein-protein interactions
Wnt 运输蛋白 Wntless 的选择性剪接及其对蛋白质-蛋白质相互作用的影响
  • DOI:
    10.1186/s12860-019-0208-1
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Jessica Petko;Mathura Thileepan;Molly Sargen;V. Canfield;R. Levenson
  • 通讯作者:
    R. Levenson
MOLECULAR DETERMINATION OF NOVEL GENES AND PATHWAYS REQUIRED FOR VESTIBULAR MORPHOGENESIS IN ZEBRAFISH
斑马鱼前庭形态发生所需的新基因和途径的分子测定
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Jessica Petko
  • 通讯作者:
    Jessica Petko
Circadian rhythms of locomotor activity in the New World tarantula Neoholothele incei (Araneae: Theraphosidae)
  • DOI:
    10.1007/s10164-025-00842-y
  • 发表时间:
    2025-04-19
  • 期刊:
  • 影响因子:
    0.800
  • 作者:
    Joseph Giulian;Natalia Toporikova;Jessica Petko;Nadia Ayoub;Thomas C. Jones;Darrell Moore
  • 通讯作者:
    Darrell Moore

Jessica Petko的其他文献

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