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CAREER: Catalytic Living Materials Constructed with Engineered Spores and Polymer Scaffolds

职业：用工程孢子和聚合物支架构建的催化活性材料

基本信息

批准号：
2237344
负责人：
Seunghyun Sim
金额：
$ 52.29万
依托单位：
University of California-Irvine
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2028-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2237344&HistoricalAwards=false
关键词：
CAREER Catalytic Living Materials Constructed

项目摘要

Non-technical summaryBacterial spores are like seeds. They are hardy, preserve well, and can grow into living organisms when the conditions are right, even if it requires waiting many years. Like a sunflower seed growing into a sunflower that produces multiple seeds, a bacterial spore can grow into billions of cells that later become billions of spores. Scientists can now engineer bacterial spores to decorate their surfaces with biological machinery called enzymes, a category of proteins that are essential to all life because of their ability to speed up useful chemical reactions. Combining these functionalized spores with engineered polymers enables the production of new materials performing catalytic reactions. This project investigates recipes for creating such materials by engineering both synthetic polymers and bacterial spores, with special emphasis on making materials degrading toxic compounds from the environment. The outcome of this research will establish a foundation for creating a diverse array of materials that can facilitate a wide range of chemical reactions. It will also provide new fundamental knowledge in building engineered interfaces between synthetic materials and bacterial spores. Along with the research activities, educational and outreach programs will be developed for community college students, K12 students, and polymer chemistry students at UC Irvine.Technical summaryThis project aims to develop robust, intelligent catalytic living materials utilizing dynamic covalent bond formation between engineered polymer scaffolds and B. subtilis spores. In particular, the team will focus on creating living materials for catalytic bioremediation. Because bacterial spores can survive harsh conditions, such as dehydration, nutrient limitation, organic solvents, and oxidative stress, spore-containing composite materials could be produced and stored in a dried form. Upon engineering both the bacterial spores and polymeric materials constituting living materials, they are expected to perform robust catalysis for a wide range of substrates, are stable under dry storage for a long period of time, and are recyclable after use. Specifically, the following four research objectives will be pursued: (1) evaluating various motifs for building a well-defined molecular interface on B. subtilis spores, (2) engineering B. subtilis spores for catalytic bioremediation, (3) synthesizing and studying physical properties of spore-containing living materials. (4) evaluating and engineering catalytic behaviors of living materials. The educational objectives to complement the research activities are (1) the development of an outreach and research program targeting local community college (CC) students and CC transfer students, (2) participation in the outreach program targeting local K12 students, and (3) mentoring and educating students at UCI and leading the polymer chemistry club activities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

细菌孢子就像种子。它们耐寒，保存良好，当条件合适时，即使需要等待多年，也能长成活的有机体。就像一颗葵花籽长成一颗能产生多个种子的葵花籽一样，一个细菌孢子可以长成数十亿个细胞，然后再变成数十亿个孢子。科学家们现在可以设计细菌孢子，用一种叫做酶的生物机械来装饰它们的表面。酶是一种蛋白质，对所有生命都是必不可少的，因为它们能够加速有用的化学反应。将这些功能化的孢子与工程聚合物相结合，可以生产出执行催化反应的新材料。该项目研究了通过工程合成聚合物和细菌孢子来制造这些材料的配方，特别强调了从环境中制造降解有毒化合物的材料。这项研究的结果将为创造各种各样的材料奠定基础，这些材料可以促进广泛的化学反应。它还将为在合成材料和细菌孢子之间建立工程界面提供新的基础知识。在开展研究活动的同时，还将为加州大学欧文分校的社区大学生、K12学生和聚合物化学专业的学生开发教育和推广项目。该项目旨在利用工程聚合物支架和枯草芽孢杆菌孢子之间形成的动态共价键，开发强大的智能催化活性材料。特别是，该团队将专注于创造用于催化生物修复的活性材料。由于细菌孢子可以在恶劣的条件下存活，如脱水、营养限制、有机溶剂和氧化应激，因此可以生产含孢子的复合材料并以干燥的形式储存。在工程上，细菌孢子和构成生命材料的聚合物材料都有望对广泛的底物发挥强大的催化作用，在干燥储存下长时间稳定，并且使用后可回收。具体而言，将实现以下四个研究目标：(1)评估各种基序以构建明确的枯草芽孢杆菌孢子分子界面；(2)对枯草芽孢杆菌孢子进行工程催化生物修复；(3)合成和研究含孢子活性材料的物理性质。(4)生物材料催化行为的评价与工程化。与研究活动相辅相成的教育目标是：(1)针对当地社区学院（CC）学生和CC转学生开展外展和研究计划，(2)参与针对当地K12学生的外展计划，(3)指导和教育UCI的学生并领导聚合物化学俱乐部活动。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。