Acquisition of Two-Color 3-D Minflux for Live-Cell Single Molecule Imaging and Tracking at Unprecedented Spatial and Temporal Resolution

采集双色 3-D Minflux，以前所未有的空间和时间分辨率进行活细胞单分子成像和跟踪

• 批准号: 2241074
• 负责人: Martin Gruebele
• 金额: $ 19.45万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-15 至 2025-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2241074&HistoricalAwards=false
• 关键词: Acquisition; Two; Color; Minflux; Live

This award will enable the purchase of a MINFLUX imaging and molecule tracking system. MINFLUX technology can locate molecules to 2 nm resolution and the ability to track molecules with 10 MHz time resolution an order of magnitude improvement over other single molecule techniques. MINFLUX is a technology developed by Nobelist, Stefan Hell. As the name suggests, MINFLUX detects individual molecules by illuminating their surroundings and not exposing the molecule itself to high light intensities, greatly reducing bleaching and phototoxicity. This is accomplished by optically activating a fluorescent molecule and then surrounding it with a donut of excitation light. As the center of the donout is moved off the molecule the fluorescence intensity increases indicating the position of the molecule. This process is then carried out in three directions locating the molecule in space while minimizing the photon flux experienced by the molecule to the range of 800 to 2500 photons per localization event.The extraordinary capabilities of this instrument will allow at least 20 faculty from 10 participating units at the University of Illinois to track receptors in neuronal synapses, image chromatin folding, neural muscular junctions, and much more. All against the background of the cell or other complex environments. There are currently two MINFLUX systems in the United States. One is located at Scripps on the west coast and one at NIH on the east coast. A MINFLUX instrument in the area would provide access to faculty members located in the Midwest and strengthen the University of Illinois’ standing in imaging.The best-in-class resolution of the MINFLUX microscope will allow new science to be conducted and foster collaborations between experimental and theoretical biophysicists to characterize these discoveries. It will be housed in the IGB Core Facility and will be managed with the same training procedures, calendars, and billing, as our other high end optical microscopes. As the MINFLUX early adopter in the Midwest, the IGB Facility will work with Abberior instruments to provide demonstrations and access for off campus researchers along with hands- on training for students and postdocs at Illinois and throughout the MidwestThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项将有助于购买 MINFLUX 成像和分子追踪系统。 MINFLUX 技术可以以 2 nm 分辨率定位分子，并且能够以 10 MHz 时间分辨率跟踪分子，比其他单分子技术提高了一个数量级。 MINFLUX 是由诺贝尔奖获得者 Stefan Hell 开发的技术。顾名思义，MINFLUX 通过照亮周围环境来检测单个分子，而不是将分子本身暴露在高光强度下，从而大大减少漂白和光毒性。这是通过光学激活荧光分子，然后用激发光甜甜圈包围它来实现的。当甜甜圈的中心移离分子时，荧光强度增加，指示分子的位置。然后，这个过程在三个方向上进行，在空间中定位分子，同时将分子经历的光子通量最小化到每次定位事件 800 至 2500 个光子的范围。该仪器的非凡功能将允许伊利诺伊大学 10 个参与单位的至少 20 名教员追踪神经元突触、图像染色质折叠、神经肌肉接头等中的受体。所有这些都是在细胞或其他复杂环境的背景下进行的。目前美国有两个 MINFLUX 系统。其中一处位于西海岸的斯克里普斯，另一处位于东海岸的国立卫生研究院。该地区的 MINFLUX 仪器将为位于中西部的教员提供接触机会，并加强伊利诺伊大学在成像领域的地位。MINFLUX 显微镜一流的分辨率将允许开展新科学，并促进实验和理论生物物理学家之间的合作，以表征这些发现。它将安置在 IGB 核心设施中，并采用与我们其他高端光学显微镜相同的培训程序、日历和计费进行管理。作为 MINFLUX 在中西部的早期采用者，IGB 设施将与 Abberior 仪器合作，为校外研究人员提供演示和访问，并为伊利诺伊州和整个中西部的学生和博士后提供实践培训。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。