Conservation genomics of the Pyrenean desman (Galemys pyrenaicus) using Next-Generation Sequencing methods.

使用下一代测序方法对比利牛斯山蜥蜴（Galemyspyrenaicus）进行保护基因组学研究。

• 批准号: 254570358
• 负责人: Dr. Oliver Hawlitschek, Ph.D.
• 金额: --
• 依托单位: Castresana lab
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/254570358?language=en
• 关键词: Conservation; genomics; Pyrenean; desman; Galemys

The proposed project focuses on the Pyrenean desman (Galemys pyrenaicus), a small semi-aquatic mammal endemic to the Iberian Peninsula. It is a focal species of the European Habitats Directive and highly threatened by extinction. Any measures for its conservation require information on its genetic diversity and phylogeographic structure. A previous study conducted by the group of Jose Castresana at the Instituto de Biología Evolutiva CSIC-UPF (IBE) in Barcelona revealed four distinct genetic lineages in the Pyrenean desman. However, it also showed that its overall genetic diversity is very low, implying that traditional sequencing methods cannot provide sufficient resolution to allow conclusions on the conservation importance of these results. In this project hosted by the IBE, Next-Generation Sequencing (NGS) methods will be used to further investigate into the population genomics of the Pyrenean desman. These methods are not yet widely established in phylogenetics, phylogeography and conservation, but may yield highly informative data for questions in which traditional DNA sequencing cannot perform properly. The applicant has finished his PhD thesis in 2013 and, considering his career stage, has already ample research experience with 14 scientific papers, mainly in molecular phylogenetics. He will receive training in NGS techniques and bioinformatics, as well as in additional skills such as scientific writing and research management, which will be highly important in the development of his career as a researcher in systematic biology. The host group has crucial knowledge in the genetics of the Pyrenean desman, genomics and bioinformatics. The results of the project are expected to promote the expected career of the applicant as a researcher in Germany by acquiring essential skills and to be a crucial contribution to the conservation of this threatened species. They will also help increasing the public awareness for the importance of the conservation of endemic European species.

拟议的项目重点是比利牛斯鱼(Galemys Pyrenaicus)，这是伊比利亚半岛特有的一种小型半水栖哺乳动物。它是欧洲栖息地指令的重点物种，受到高度灭绝的威胁。任何保护它的措施都需要关于它的遗传多样性和系统地理结构的信息。巴塞罗那生物进化研究所(IBE)的Jose Castresana团队之前进行的一项研究显示，比利牛斯人有四种不同的遗传谱系。然而，它也表明它的总体遗传多样性非常低，这意味着传统的测序方法不能提供足够的分辨率来得出这些结果的保护重要性的结论。在这项由国际教育局主持的项目中，将使用下一代测序(NGS)方法进一步研究比利牛斯德曼的种群基因组学。这些方法在系统发育学、系统地理学和保护方面还没有得到广泛的确立，但对于传统DNA测序无法正确执行的问题，这些方法可能会产生高度信息的数据。申请人已于2013年完成博士论文，考虑到他的职业生涯阶段，已经有丰富的研究经验，发表了14篇科学论文，主要是分子系统发育学。他将接受NGS技术和生物信息学方面的培训，以及科学写作和研究管理等其他技能方面的培训，这些技能对他作为系统生物学研究人员的职业发展将非常重要。东道主群体在比利牛斯迪曼的遗传学、基因组学和生物信息学方面拥有至关重要的知识。该项目的成果预计将通过获得基本技能来促进申请者在德国作为研究人员的预期职业生涯，并将对保护这一受威胁物种作出重要贡献。它们还将有助于提高公众对保护欧洲特有物种的重要性的认识。