Excellence in Research: Development of a Fluorescent Assay to Measure LAT1 Transport
卓越的研究:开发测量 LAT1 转运的荧光测定法
基本信息
- 批准号:2301301
- 负责人:
- 金额:$ 55.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-15 至 2026-08-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
With the support of the Chemistry of Life Processes Program in the Chemistry Division and the Historically Black Colleges and Universities Excellence in Research (HBCU-EiR) Program, Dr. Jacqueline Smith will investigate the transport of small molecules through native transporters. Cell survival depends on the delivery of nutrients such as amino acids, carbohydrates and other valuable biochemicals into the cell. The delivery of the nutrients is enabled by transporters, which are proteins located in the cell membrane. The study of these proteins is challenging. Dr. Smith’s research seeks to create novel chemical probes to study a transporter, specifically the large amino acid transporter (LAT1). The research will also focus on the development of a method for rapid identification of small molecules that are substrates for LAT1. This project will give students at Bowie State an opportunity to explore protein biochemistry and other modern chemical biology techniques. The large amino acid transporter (LAT1) is a key nutrient transporter that is highly expressed in the cells in the brain and placenta and in cells with high energy demand in general. As a transmembrane protein, the structure of LAT1 has been determined only recently and transport by LAT1 was traditionally measured using radioisotope assays. Although computational methods have been used to study LAT1, experimental methods for validation of the computational predictions are lacking. Dr. Smith will pursue the development of proteoliposomes that contain LAT1. The small molecule binding to LAT1 within the proteoliposomes will be studied by surface plasmon resonance (SPR). Newly developed fluorescent substrates will be used to screen for molecules that can be transported through LAT1. The tools developed in this study will be applied to gain a better understanding of the binding to and transport of small molecules by LAT1.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在化学系生命过程化学项目和历史上黑人高等院校卓越研究项目(HBCU-EiR)的支持下,杰奎琳·史密斯博士将研究小分子通过天然转运体的运输。细胞的存活依赖于氨基酸、碳水化合物和其他有价值的生化物质等营养物质进入细胞。营养物质的输送是由转运蛋白实现的,转运蛋白是位于细胞膜上的蛋白质。对这些蛋白质的研究具有挑战性。史密斯博士的研究旨在创造新的化学探针来研究转运蛋白,特别是大氨基酸转运蛋白(LAT1)。该研究还将侧重于开发一种快速识别作为LAT1底物的小分子的方法。这个项目将为鲍伊州立大学的学生提供一个探索蛋白质生物化学和其他现代化学生物学技术的机会。大氨基酸转运蛋白(large amino acid transporter, LAT1)是一种关键的营养转运蛋白,在脑和胎盘细胞以及一般高能量需求细胞中高度表达。作为一种跨膜蛋白,LAT1的结构直到最近才被确定,并且LAT1的运输传统上使用放射性同位素测定。虽然计算方法已用于研究LAT1,但缺乏验证计算预测的实验方法。史密斯博士将继续研究含有LAT1的蛋白脂质体。利用表面等离子体共振(SPR)研究蛋白脂质体内与LAT1结合的小分子。新开发的荧光底物将用于筛选可通过LAT1运输的分子。本研究开发的工具将用于更好地了解LAT1与小分子的结合和运输。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jacqueline Smith其他文献
Dance Composition: A Practical Guide for Teachers
舞蹈创作:教师实用指南
- DOI:
- 发表时间:
1978 - 期刊:
- 影响因子:0
- 作者:
Jacqueline Smith - 通讯作者:
Jacqueline Smith
The Aspergillus nidulans cnxABC Locus Is a Single Gene Encoding Two Catalytic Domains Required for Synthesis of Precursor Z, an Intermediate in Molybdenum Cofactor Biosynthesis*
构巢曲霉 cnxABC 基因座是一个单基因,编码合成前体 Z(钼辅因子生物合成的中间体)所需的两个催化结构域*
- DOI:
- 发表时间:
1997 - 期刊:
- 影响因子:4.8
- 作者:
S. Unkles;Jacqueline Smith;G. Kanan;L. Millar;I. Heck;D. Boxer;J. R. Kinghorn - 通讯作者:
J. R. Kinghorn
Neonatal electronic resuscitation app documentation (NeRD): Electronic documentation compared to written documentation
新生儿电子复苏应用程序文档 (NeRD):电子文档与书面文档的比较
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
Jacqueline Smith;Asher Jakobsson;Victoria J Kain;Y. Kandasamy;Rosie Bragg - 通讯作者:
Rosie Bragg
Integration of the genetic and physical maps of the chicken macrochromosomes.
鸡大染色体遗传图谱和物理图谱的整合。
- DOI:
10.1046/j.1365-2052.2000.00549.x - 发表时间:
2000 - 期刊:
- 影响因子:2.4
- 作者:
Jacqueline Smith;I. Paton;C. Bruley;D. Windsor;D. Burt;F. A. P. León;D. Burke - 通讯作者:
D. Burke
Whole genome sequencing of three native chicken varieties (Common Deshi, Hilly and Naked Neck) of Bangladesh
孟加拉国三个本土鸡品种(普通德西鸡、丘陵鸡和裸颈鸡)的全基因组测序
- DOI:
10.1038/s41597-024-04291-z - 发表时间:
2024-12-24 - 期刊:
- 影响因子:6.900
- 作者:
Md Ataul Goni Rabbani;Adriana Vallejo-Trujillo;Zhou Wu;Katarzyna Miedzinska;Shakila Faruque;Kellie A. Watson;Jacqueline Smith - 通讯作者:
Jacqueline Smith
Jacqueline Smith的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jacqueline Smith', 18)}}的其他基金
CAREER: Modulating cell signaling and migration by targeted small-molecule binding to a key regulator of protein degradation
职业:通过与蛋白质降解的关键调节因子结合的靶向小分子来调节细胞信号传导和迁移
- 批准号:
2239475 - 财政年份:2023
- 资助金额:
$ 55.76万 - 项目类别:
Continuing Grant
Understanding the resilience of wild birds to climate change: seasonal genomics of the annual migratory breeding cycle
了解野生鸟类对气候变化的适应能力:年度迁徙繁殖周期的季节性基因组学
- 批准号:
BB/V001647/1 - 财政年份:2021
- 资助金额:
$ 55.76万 - 项目类别:
Research Grant
Research Initiation Award: Microwave Synthesis of Imidazole Compounds to Investigate Allosteric Binding to VCP
研究启动奖:微波合成咪唑化合物以研究 VCP 的变构结合
- 批准号:
2000028 - 财政年份:2020
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Research Initiation Award: Novel Imidazole Compounds to Investigate the Unfolded Protein Response (UPR)
研究启动奖:用于研究未折叠蛋白反应 (UPR) 的新型咪唑化合物
- 批准号:
1800165 - 财政年份:2018
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Faculty Development for Technician Education in Welding, Materials Joining, and Non-Destructive Testing
焊接、材料连接和无损检测技术人员教育师资队伍建设
- 批准号:
1400530 - 财政年份:2014
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Identification of the molecular basis of differential host responses to rapidly evolving Avian Influenza viruses in different avian species
鉴定不同鸟类物种对快速进化的禽流感病毒的不同宿主反应的分子基础
- 批准号:
BB/L004666/1 - 财政年份:2014
- 资助金额:
$ 55.76万 - 项目类别:
Research Grant
Computer Science, Engineering, Mathematic Scholarship Program
计算机科学、工程、数学奖学金计划
- 批准号:
9987343 - 财政年份:2000
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
相似国自然基金
Research on Quantum Field Theory without a Lagrangian Description
- 批准号:24ZR1403900
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
Cell Research
- 批准号:31224802
- 批准年份:2012
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research
- 批准号:31024804
- 批准年份:2010
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research (细胞研究)
- 批准号:30824808
- 批准年份:2008
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
- 批准号:10774081
- 批准年份:2007
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
Collaborative Research: Broadening Instructional Innovation in the Chemistry Laboratory through Excellence in Curriculum Development
合作研究:通过卓越的课程开发扩大化学实验室的教学创新
- 批准号:
2337028 - 财政年份:2024
- 资助金额:
$ 55.76万 - 项目类别:
Continuing Grant
Collaborative Research: Broadening Instructional Innovation in the Chemistry Laboratory through Excellence in Curriculum Development
合作研究:通过卓越的课程开发扩大化学实验室的教学创新
- 批准号:
2337027 - 财政年份:2024
- 资助金额:
$ 55.76万 - 项目类别:
Continuing Grant
Excellence in Research: Developmental regulation of WDR77 coordinates prostate growth and differentiation during the development through puberty
卓越研究:WDR77 的发育调节可协调青春期发育期间的前列腺生长和分化
- 批准号:
2300390 - 财政年份:2023
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Excellence in Research: An investigation of the effects of Nicandra physalodes on the growth and development of weeds
卓越研究:Nicandra physalodes 对杂草生长发育影响的调查
- 批准号:
2302684 - 财政年份:2023
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Excellence in Research: The role of Neuregulin-1 in proliferation and differentiation of brain cortical neuronal stem cells during fetal development
卓越研究:Neuregulin-1 在胎儿发育过程中脑皮质神经元干细胞增殖和分化中的作用
- 批准号:
2200534 - 财政年份:2022
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
South Eastern Mediterranean Excellence Development in FIre REsearch (SEMEDFIRE)
东南地中海火灾研究卓越发展 (SEMEDFIRE)
- 批准号:
10058103 - 财政年份:2022
- 资助金额:
$ 55.76万 - 项目类别:
EU-Funded
Excellence in Research: Human Visual Perception of Changes in Smoke and Flame Cues during Early Fire Development
卓越的研究:早期火灾发展过程中烟雾和火焰线索变化的人类视觉感知
- 批准号:
2200416 - 财政年份:2022
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Excellence in Research: Development of two-dimensional (2D) molybdenum disulfide (MoS2) and molybdenum selenium (MoSe2) thin-film nanomaterials and nanoelectronic devices
卓越研究:二维(2D)二硫化钼(MoS2)和钼硒(MoSe2)薄膜纳米材料和纳米电子器件的开发
- 批准号:
2100748 - 财政年份:2021
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Excellence in Research: Development of a Non-Fluorinated Etching Procedure to Prepare MXene Hybrid Materials for Composite Applications
卓越研究:开发非氟化蚀刻工艺来制备用于复合材料应用的 MXene 混合材料
- 批准号:
2101001 - 财政年份:2021
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant
Collaborative Research: Excellence in Research: Impact of Gbx2 on neural crest cells during neuronal, craniofacial and cardiovascular development
合作研究:卓越研究:Gbx2 对神经元、颅面和心血管发育过程中神经嵴细胞的影响
- 批准号:
1956450 - 财政年份:2020
- 资助金额:
$ 55.76万 - 项目类别:
Standard Grant