Heterogeneity of transmitter receptor-linked Ca2+ signals in astrocytes II

星形胶质细胞中递质受体连接的 Ca2 信号的异质性 II

• 批准号: 255302235
• 负责人: Professor Dr. Frank Kirchhoff
• 金额: --
• 依托单位: Center for Integrative Physiology and Molecular Medicine (CIPMM)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/255302235?language=en
• 关键词: Heterogeneity; transmitter; receptor; linked; Ca2+

Astrocytes represent a main cell population in the central nervous system. Although they are electrically non-excitable, they display a complex spatial and temporal pattern of Ca2+ signals. The complexity is not only restricted to the intracellular distribution, but varies also among adjacent astrocytes or astrocyte subpopulation. Here, we will study the modulation of these Ca2+ signals by astrocyte-expressed receptors that are responsive to a range of excitatory, inhibitory and modulatory transmitters. Inducible and astrocyte-specific gene recombination in combination with in vivo two-photon laser-scanning microscopy and electrophysiology will be employed to study the impact of the purinergic receptor subunit P2Y1, the NMDA-type glutamate receptor NR1, the GABAB receptor GB1 and the adenosine A1 receptor on mouse brain function and behavior.

星形胶质细胞是中枢神经系统的主要细胞群。虽然它们是电不可兴奋的，但它们显示出复杂的Ca2+信号的空间和时间模式。这种复杂性不仅限于细胞内分布，而且在相邻星形胶质细胞或星形胶质细胞亚群之间也存在差异。在这里，我们将研究星形胶质细胞表达的受体对一系列兴奋性，抑制性和调节性递质的反应，这些Ca2+信号的调制。诱导和星形胶质细胞特异性基因重组结合体内双光子激光扫描显微镜和电生理学将被用来研究嘌呤能受体亚基P2Y1，NMDA型谷氨酸受体NR1，GABAB受体GB1和腺苷A1受体对小鼠脑功能和行为的影响。