NSF PRFB FY23: Cross-species gene regulation of a plant-fungal symbiosis under environmental stress

NSF PRFB FY23：环境胁迫下植物-真菌共生的跨物种基因调控

• 批准号: 2305481
• 负责人: Damian Hernandez
• 金额: $ 24万
• 依托单位: Hernandez, Damian
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-01 至 2027-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2305481&HistoricalAwards=false
• 关键词: NSF; PRFB; FY23; Cross; species

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2023, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment, and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. About 75% of all land plants partner with just one type of fungus, "arbuscular mycorrhizal fungi". This relationship shapes how plants get nutrients and how whole ecosystems function. Whether this relationship is useful for plants depends on environmental factors like nutrient availability. For example, fungi often help plants when nutrients are scarce, but can harm plants when plants can easily get nutrients on their own. Unfortunately, the genes that control when this relationship harms or helps plants are not well-known. Knowing which genes determine when fungi benefit plants will be vital to using fungi in agriculture and biodiversity efforts. The fellow will use large gene surveys, greenhouse experiments, and microscopy to explore how plants and fungi impact each other’s genes under different conditions. The fellow also proposes broadening participation in biology by continuing to mentor students from diverse backgrounds and organizing career development seminars for students. These seminars will provide necessary knowledge for applying to careers in STEM.The fellow will identify genetic regulation occurring across species (i.e., plant genes that regulate fungal genes and vice versa) to understand how plants and fungi co-regulate their relationship in different environmental conditions. The fellow will satisfy this objective in three aims: 1) the fellow will explore how environmental stress reorganizes co-expression patterns of plant and fungal genes using gene co-expression networks, 2) the fellow will use plant growth assays of the model legume Medicago trunculata missing potentially important regulatory genes (i.e., genes with many connections in the above networks) with/without arbuscular mycorrhizal fungi under different stresses, and, lastly, 3) the fellow will grow Medicago truncatula with 19 different characterized fungal isolates to explore how genetic/functional diversity of fungal partners impacts context-dependency of plant-mycorrhizal associations. Through the completion of this project and working closely with Drs. John Stinchcombe and Vasilis Kokkoris, the fellow will: 1) refine his training in next-generation sequencing and multivariate statistics, 2) gain skills in fungal biology and fungal assays, and 3) develop as a mentor through teaching biology courses and developing courses through his professional development seminar series.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动为2023财年生物学的NSF博士后研究奖学金提供了资金，该研究综合研究调查了有关基因组，环境和表型之间相互作用的生活规则。奖学金支持对研究员的研究和培训，这些研究和培训将以创新的方式为生活规则做出贡献。在所有土地植物中，大约有75％的植物与一种真菌合作，“羊膜菌根真菌”。关系塑造植物如何获得营养以及整个生态系统的运作方式。这种关系是否对植物有用取决于养分可用性等环境因素。例如，真菌经常在营养稀缺时帮助植物，但是当植物很容易单独获取营养时，可能会伤害植物。不幸的是，控制这种关系何时损害或帮助植物的基因并不众所周知。知道哪些基因决定了何时使真菌受益于植物对在同意和生物多样性努力中使用真菌至关重要。该研究员将使用大型基因调查，温室实验和显微镜来探索植物和真菌在不同条件下如何影响彼此的基因。该研究员还提议通过继续对来自潜水员背景的精神学生并为学生组织职业发展中的精神学生，扩大生物学的参与。这些SEMIR将提供必要的知识，以应用于STEM中的职业。同伴将确定跨物种（即调节真菌基因的植物基因，反之亦然）发生的遗传调节，以了解植物和真菌如何在不同环境条件下共同调节其关系。同伴将以三个目标满足这一目标：1）伙伴们将探索环境压力如何使用基因共表达网络重新组织植物和真菌基因的共表达模式，2）同伴将使用模型的豆科植物生长测定法的植物生长测定法的植物生长测定法对缺失潜在的调节基因的基因（即，与以上网络相关的基因）的差异（即，在上面的许多范围内都不存在，arb）压力，最后3）该研究员将用19种不同特征的真菌分离株种植Medicago truncatula，以探索真菌伴侣的遗传/功能多样性如何影响植物 - 肌肉关联的上下文依赖性。通过完成该项目并与DRS紧密合作。约翰·斯汀奇科姆（John Stinchcombe）和瓦西利斯·科科里斯（Vasilis Kokkoris），同伴：1）在下一代测序和多元统计中完善他的培训，2）获得真菌生物学和真菌分析的技能，以及3）3）三）通过他的专业发展奖项来通过他的专业发展奖，并通过nsf的指定来发展，并通过教学课程来发展，并以反映nsf的代表来发展。智力优点和更广泛的影响审查标准。