NSF PRFB FY 2023: Does long-term selection for growth on recalcitrant vs labile carbon impact Streptomyces nutrient use or inhibitory phenotypes?

NSF PRFB 2023 财年：顽固性碳与不稳定碳的长期选择是否会影响链霉菌的营养利用或抑制表型？

• 批准号: 2305753
• 负责人: Brett Lane
• 金额: $ 24万
• 依托单位: Lane, Brett
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2305753&HistoricalAwards=false
• 关键词: NSF; PRFB; FY; 2023; Does

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2023, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment, and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. This project will explore the impact of soil carbon complexity on microbial growth and interactions between microbes competing for limited carbon resources. Soil carbon can be broadly categorized as either readily available for microbial consumption (labile carbon) or difficult for microbial populations to metabolize (recalcitrant carbon). In order to utilize recalcitrant carbon, microbes must first break these complex compounds into their building blocks while also defending these building blocks from theft by neighboring microbes. To this end, microbes which specialize in the metabolism of recalcitrant carbon utilize antimicrobial compounds to prevent their neighbors from stealing these resources. These antimicrobial compounds are also believed to play a significant role in the suppression of soil-inhabiting, plant-pathogenic, organisms. As rising global temperatures are anticipated to increase the relative proportion of recalcitrant carbon within the soil, soil-inhabiting microbes are anticipated to alter their production of and resistance to antimicrobial compounds to further their ability to compete for these complex resources. Through this fellowship, the PI will further our understanding of how the adaptation of microbes to increasingly prominent recalcitrant carbon will influence interactions between soil-inhabiting microbes. The results of this research will be vital in promoting soil health and will ensure the protection of food crops from soil-inhabiting pathogens. Furthermore, this fellowship will further the training of the fellow, research personnel, graduate, and undergraduate students at the University of Minnesota as well as promote the participation and training of underrepresented groups in science.In this research, isolates of Streptomyces, a genus of prominent, soil-borne, antibiotic-producing bacteria, will undergo long-term selection for growth in petridishes containing a sole carbon source of varying recalcitrance. Isolates will be dotted on plates and grown until they exhibit sporulation. Spores will then be transferred to subsequent plates for a total of fifty generations of selection on either recalcitrant or labile carbon. It is anticipated that growth on recalcitrant carbon sources, in contrast to labile carbon, will impart selection for the utilization of a wider range of carbon sources and will increase the frequency and intensity of constitutive antibiotic production. Following fifty generations of growth, the fellow will characterize changes in isolate nutrient use and inhibitory phenotypes as well as genomic and transcriptional changes associated with carbon metabolism and secondary metabolite production. The results of this project will provide insight into the genomic and transcriptional modifications resulting from selection for growth on carbon sources of varying recalcitrancy. This project will further the fellow’s training in microbial ecology and genomic analysis as well as mentorship and scientific communication. The fellow will also recruit undergraduate researchers to train the next generation of scientists. In addition, the fellow will develop and conduct outreach events to broaden K-12 participation in science.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动资助了美国国家科学基金会2023财年生物学博士后研究奖学金，研究基因组，环境和表型之间相互作用的生命规则的综合研究。该奖学金支持将以创新方式对生活规则领域作出贡献的研究员的研究和培训。该项目将探讨土壤碳复杂性对微生物生长的影响以及微生物之间争夺有限碳资源的相互作用。土壤碳可以大致分为易于微生物消耗的碳（不稳定碳）和难以微生物代谢的碳（顽固性碳）。为了利用顽固性碳，微生物必须首先将这些复杂的化合物分解成它们的组成部分，同时保护这些组成部分不被邻近的微生物窃取。为此，专门代谢顽固性碳的微生物利用抗菌化合物来防止它们的邻居窃取这些资源。这些抗微生物化合物也被认为在抑制土壤生物、植物病原生物方面起着重要作用。由于预计全球气温上升将增加土壤中顽固性碳的相对比例，预计土壤微生物将改变其对抗微生物化合物的生产和耐药性，以进一步提高它们争夺这些复杂资源的能力。通过这项研究，PI将进一步了解微生物对日益突出的顽固性碳的适应将如何影响土壤微生物之间的相互作用。这项研究的结果对促进土壤健康至关重要，并将确保保护粮食作物免受土壤病原体的侵害。此外，该奖学金将进一步培训明尼苏达大学的研究员、研究人员、研究生和本科生，并促进未被充分代表的科学群体的参与和培训。在这项研究中，链霉菌，一个突出的，土壤传播的，产生抗生素的细菌属，将经过长期的选择，在含有不同顽固性的唯一碳源的培养皿中生长。分离株将被散布在培养皿上并生长，直到它们产生孢子。然后将孢子转移到随后的培养皿中，在顽固碳或不稳定碳上进行总共50代的选择。据预测，与不稳定碳相比，顽固性碳源的生长将为更广泛的碳源的利用提供选择，并将增加组成型抗生素生产的频率和强度。经过50代的生长，该研究员将描述分离物营养利用和抑制表型的变化，以及与碳代谢和次级代谢物产生相关的基因组和转录变化。这个项目的结果将提供对基因组和转录修饰的深入了解，这些修饰是由对不同顽固性碳源的生长选择引起的。该项目将进一步促进研究员在微生物生态学和基因组分析方面的培训，以及指导和科学交流。该研究员还将招募本科研究人员来培养下一代科学家。此外，该研究员将开发和开展外展活动，以扩大K-12学生对科学的参与。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。