Collaborative Research: Mechanisms driving positive and negative tree-fungal feedbacks across an abiotic-stress gradient

合作研究：在非生物胁迫梯度上驱动正负树真菌反馈的机制

• 批准号: 2310100
• 负责人: Joseph LaManna
• 金额: $ 44万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2310100&HistoricalAwards=false
• 关键词: Collaborative; Research; Mechanisms; driving; positive

Understanding how species interactions affect biological diversity has long been of interest to ecologists. Ecological theory and observations have shown that negative interactions between adult trees and seedlings of the same species stabilize populations, prevent local extinction, and maintain biological diversity. Alternatively, adult trees can increase the growth and survival of younger seedlings of the same species, particularly in stressful environments, which in turn can lead to lower levels of biological diversity. However, the role of pathogens and mutualists in mediating these interactions and giving rise to variation in plant diversity across environmental gradients remains unknown. This research funds a collaboration between plant and fungal population and community ecologists to uncover the role of positive and negative above and below ground plant-microbial interactions in the maintenance of species diversity. It will further explore how changes in environmental stress might impact tree diversity in forest ecosystems. This research will inform and engage local and regional land managers and stakeholders of the role of above and below ground mutualists in the growth and survival of key tree species in the Pacific Northwest.As the global climate shifts, research into how populations and communities adapt to environmental changes has become increasingly critical. Microbes and their interactions with plants, both positive and negative, play a underexamined role in determining species distributions, abundances, and their expected responses to climate-induced stress. As a result, we lack a comprehensive understanding of how microbially mediated interactions, and in particular above and below ground conspecific feedbacks within and among plant species, change in their strength and direction across environmental stress gradients. The research funded by this award will investigate how fungal symbionts contribute to tree species abundance, distribution, and diversity along stress gradients, both directly and indirectly, by influencing density-dependent interactions and feedbacks among adult and juvenile trees. Researchers will test the hypothesis that the net effect of fungal symbionts on plant performance is correlated with the level of abiotic drought and nutrient stress in the environment, with conspecific feedback shifting from negative to positive as abiotic stress increases. First, in a field experiment at the HJ Andrews Long Term Ecological Research (LTER) site, investigators will reciprocally transplant three focal tree species beneath the canopies of adult conspecific or heterospecific trees along natural moisture and soil-nutrient stress gradients, manipulating fungal mutualists to evaluate their effect on conspecific density-dependent feedback. In greenhouse experiments, trees, microbes, and soil nutrient and drought stress will be manipulated. Finally, this project will integrate host-symbiont dynamics into regional models of forest community dynamics to assess how tree population/community dynamics might be altered under conditions of abiotic stress. Overall, the proposed work will test multiple hypotheses regarding how fungal symbionts contribute to the direction and strength of feedback for dominant tree species in forests of the Pacific Northwest.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

了解物种间的相互作用如何影响生物多样性一直是生态学家的兴趣所在。生态学理论和观察表明，同一物种的成年树木和幼苗之间的负面相互作用可以稳定种群，防止局部灭绝，并保持生物多样性。另外，成年树木可以增加同一物种的幼苗的生长和存活，特别是在压力环境中，这反过来又会导致生物多样性水平降低。然而，病原体和互利共生体在介导这些相互作用和引起跨环境梯度的植物多样性变化中的作用仍然未知。这项研究资助了植物和真菌种群与社区生态学家之间的合作，以揭示地上和地下植物-微生物相互作用在维持物种多样性方面的积极和消极作用。它将进一步探讨环境压力的变化如何影响森林生态系统中的树木多样性。这项研究将告知和参与当地和区域土地管理者和利益相关者的作用，地上和地下的互惠在太平洋西北地区的主要树种的生长和生存。随着全球气候的变化，研究人口和社区如何适应环境变化变得越来越重要。微生物及其与植物的相互作用，无论是积极的还是消极的，在确定物种分布，丰度和它们对气候引起的压力的预期反应方面发挥着未被充分研究的作用。因此，我们缺乏对微生物介导的相互作用，特别是植物物种内部和之间的地上和地下同种反馈如何在环境胁迫梯度上改变其强度和方向的全面了解。该奖项资助的研究将调查真菌共生体如何直接和间接地通过影响成年树和幼龄树之间的密度依赖性相互作用和反馈，沿着沿着应力梯度对树种丰度、分布和多样性做出贡献。研究人员将测试这一假设，即真菌共生体对植物性能的净效应与环境中的非生物干旱和营养胁迫水平相关，随着非生物胁迫的增加，同种反馈从负向正转变。首先，在HJ安德鲁斯长期生态研究（LTER）网站的实地实验中，调查人员将三个焦点树种下的树冠成年同种或异种树沿着自然水分和土壤养分压力梯度，操纵真菌互惠评估其对同种密度依赖的反馈效果。在温室实验中，树木，微生物，土壤养分和干旱胁迫将被操纵。最后，本项目将把宿主-共生体动态纳入森林群落动态的区域模型，以评估在非生物胁迫条件下树木种群/群落动态可能如何改变。总的来说，拟议的工作将测试多个假设，关于真菌共生体如何有助于在太平洋西北地区的森林中的优势树种的反馈的方向和强度。这个奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。