Functional characterization of basophils in vivo

嗜碱性粒细胞与过敏反应和对抗蠕虫的保护性免疫有关。嗜碱性粒细胞的发育和效应子功能特征不明确。利用逆转录病毒转导和过继转移嗜碱性粒细胞和造血干细胞，我们将研究体内嗜碱性粒细胞的发育和迁移。我们将进一步使用来自嗜碱性粒细胞缺陷小鼠和不同基因敲除小鼠的细胞产生的混合骨髓嵌合体来研究嗜碱性粒细胞在过敏性皮肤反应和针对不同蠕虫的保护性免疫中的作用。该项目将提高嗜碱性粒细胞生物学的知识，并可能有助于开发新的治疗过敏性疾病的治疗策略。

Basophils are associated with allergic reactions and protective immunity against helminths. Development and effector functions of basophils are poorly characterized. Using retroviral transductions and adoptive transfers of basophils and hematopoietic stem cells we will study the development and migration of basophils in vivo. We will further use mixed bone marrow chimeras generated with cells from basophil-deficient mice and different knock-out mice to study the role of basophils for allergic skin reactions and protective immunity against different helminths. The proposed project will improve the knowledge about basophil biology and may serve to develop new therapeutic strategies for treatment of allergic disorders.