BRC-BIO: Pick your poison: The physiological cost of alkaloid sequestration in dendrobatid poison frogs

BRC-BIO：选择你的毒药：树突状毒蛙中生物碱封存的生理成本

• 批准号: 2312591
• 负责人: Vincent Farallo
• 金额: $ 48.89万
• 依托单位: University of Scranton
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2312591&HistoricalAwards=false
• 关键词: BRC; BIO; Pick; your; poison

Organisms typically have physiologies adapted to the environments that they inhabit. However, many habitats are rapidly changing due to human activities and climate change. These changes can impact species’ ability to persist in previously adequate environments. Poison frogs in the family Dendrobatidae are charismatic because of their bright colors and unique ability to sequester alkaloid defenses from their natural diet of mites and ants. The frogs use these alkaloids as a chemical defense against predators and microbes, yet little is known about the physiological costs associated with developing these defenses. More specifically, how does alkaloid sequestration influence the energy requirements for individuals to survive? And in turn, how does this impact how frogs interact with their environment such as how much time they need to spend foraging for food or finding a mate? Frogs are poikilothermic, which means changes to environmental temperature will directly impact their body temperature. As temperature increases, so does metabolic rate, which will require frogs to acquire more energy (for example, eat more food) or utilize different thermal microhabitats. Therefore, increases in temperature due to climate change have the potential to increase the cost of alkaloid sequestration for frogs. The current project aims to establish a career study system, rooted in involving undergraduate students and community members. Undergraduate students will be trained in cutting edge techniques for studying physiology and chemical ecology. The charismatic nature of the frogs will also allow for outreach events, engaging the community to learn about ecophysiology and conservation. Amphibians are especially susceptible to climate change as their physiologies, specifically respiration and other aspects of metabolism, are dependent on specific temperature and moisture conditions. Neotropical poison frogs in the family Dendrobatidae also have specialized physiologies that allow them to sequester dietary alkaloids to be used as chemical defenses. The goal of this BRC-BIO project is to quantify how alkaloid sequestration by dendrobatid frogs impacts ecologically relevant physiological processes, such as metabolism. To accomplish this goal, a breeding colony of 10 species of dendrobatids will be established at The University of Scranton, which will be used to test three primary hypotheses: (1) sequestration of alkaloids has a physiological cost that will result in increased metabolic rate and energy expenditure; (2) changes in metabolic rate will vary by frog lineage, as alkaloid sequestration has evolved multiple times within the family; and (3) metabolic rate will increase proportionally with the quantity of alkaloid sequestered. These hypotheses will be tested by providing frogs with a control or alkaloid-supplemented diet and quantifying individual frog’s metabolic rate by measuring and comparing rates of carbon dioxide production and oxygen consumption. Dendrobatids naturally possess more than 20 different types of alkaloids, and therefore a diversity of alkaloids and their effects on physiology will be measured. The data generated by this BRC-BIO project, in conjunction with the development of a frog breeding colony, will continue to support additional questions with an emphasis on student-led independent projects, novel physiology experiments in undergraduate courses, and outreach with the community and K-12 schools.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生物体通常具有适应它们所居住的环境的生理学。然而，由于人类活动和气候变化，许多栖息地正在迅速变化。这些变化可能会影响物种在以前适当的环境中生存的能力。毒蛙在家庭Dendrobatidae是魅力，因为他们明亮的颜色和独特的能力，隔离生物碱防御从他们的自然饮食螨和蚂蚁。青蛙使用这些生物碱作为对抗捕食者和微生物的化学防御，但人们对发展这些防御的生理成本知之甚少。更具体地说，生物碱螯合如何影响个体生存的能量需求？反过来，这又如何影响青蛙与环境的互动，比如它们需要花多少时间觅食或寻找配偶？青蛙是变温动物，这意味着环境温度的变化会直接影响它们的体温。随着温度的升高，代谢率也会增加，这将需要青蛙获得更多的能量（例如，吃更多的食物）或利用不同的热微生境。因此，由于气候变化导致的温度升高有可能增加青蛙生物碱螯合的成本。目前的项目旨在建立一个职业研究系统，植根于大学生和社区成员的参与。本科生将接受尖端技术的培训，以研究生理学和化学生态学。青蛙的魅力也将允许外展活动，参与社区了解生态生理学和保护。两栖动物特别容易受到气候变化的影响，因为它们的生理，特别是呼吸和代谢的其他方面，取决于特定的温度和湿度条件。新热带毒蛙科也有专门的生理学，使他们能够螯合食物生物碱用作化学防御。这个BRC-BIO项目的目标是量化石斛蛙的生物碱螯合如何影响生态相关的生理过程，如新陈代谢。为了实现这一目标，将在斯克兰顿大学建立一个10种树枝蛙的繁殖群，将用于测试三个主要假设：（1）生物碱的螯合具有生理成本，这将导致代谢率和能量消耗增加;（2）代谢率的变化将因青蛙谱系而异，因为生物碱螯合在家族中经历了多次进化;（3）代谢率随生物碱螯合量的增加而增加。这些假设将通过向青蛙提供对照或补充维生素D的饮食进行测试，并通过测量和比较二氧化碳产生和耗氧量来量化个体青蛙的代谢率。树枝虫天然具有20多种不同类型的生物碱，因此将测量生物碱的多样性及其对生理的影响。这个BRC-BIO项目产生的数据，结合青蛙繁殖群的开发，将继续支持额外的问题，重点是学生主导的独立项目，本科课程中的新颖生理学实验，与社区和K的联系该奖项反映了NSF的法定使命，并通过使用基金会的智力价值进行评估，被认为值得支持和更广泛的影响审查标准。