Role and function of microRNAs within microvesicles in the regulation of vascular biology

微囊泡(microvesicles，MV)是细胞在生理和病理条件下释放出来的膜小泡。微泡由三个亚类组成：外体(Ex，40-100 nm)、微粒子(MP，0.1µm-1µm)和凋亡小体(AB，1µm-4µm)。所有三个来自内皮细胞的MV亚群都含有并在细胞之间转移功能性microRNAs(MiRs)，从而调节血管保护和再生。在这项建议中，我们的目标是评估如何在生理和病理条件下将细胞中的选择性miR包装成MV亚群。此外，我们的目标是探索MV内的miRs如何影响体内和体外的血管平滑肌细胞(VSMC)生物学。最后，我们的目标是详细研究miR-126在不同血管损伤模型中的作用，以表征其在新生血管形成和再内皮化中的作用。通过这项建议，我们的目标是提供更多关于MV相关MIR的知识，以便在未来可能将MV中的MIR用作治疗工具。

Microvesicles (MV) are small membrane vesicles which are released from cells under physiological and pathological conditions. Microvesicles consists of three subgroups: exosomes (Ex, 40-100nm), microparticles (MP, 0,1µm-1µm) and apoptotic bodies (AB, 1µm-4µm). All three MV subgroups derived from endothelial cells were shown to contain and transfer functional microRNAs (miRs) between cells and thereby regulating vascular protection and regeneration. Within this proposal, we aim to asses how the packaging of selective miRs from cells into MV subgroups is regulated under physiological and pathological conditions. Furthermore, we aim to explore how miRs within MV influence vascular smooth muscle cell (VSMC) biology in vivo and in vitro. Finally, we aim to investigate in detail the role of miR-126 in different vascular injury models to characterize its role in neointimaformation and reendothelialization. With this proposal, we aim to contribute further knowledge about MV-associated miRs in order to probably use miRs within MV in the future as a therapeutic tool.