Role and function of microRNAs within microvesicles in the regulation of vascular biology

微囊泡（Microvesicles，MV）是在生理和病理条件下由细胞释放的小的膜泡。微泡由三个亚组组成：外来体（Ex，40- 100 nm），微粒（MP，0.1 µm-1µm）和凋亡小体（AB，1µm-4µm）。来自内皮细胞的所有三个MV亚组均显示含有并在细胞之间转移功能性microRNA（miR），从而调节血管保护和再生。在这个提议中，我们的目标是评估如何包装的选择性miR从细胞到MV亚组在生理和病理条件下进行调节。此外，我们的目的是探讨如何在MV的miR影响血管平滑肌细胞（VSMC）的生物学在体内和体外。最后，我们的目标是详细研究miR-126在不同血管损伤模型中的作用，以表征其在新内膜形成和再内皮化中的作用。通过这一提议，我们的目标是进一步了解MV相关的miR，以便将来可能在MV中使用miR作为治疗工具。

Microvesicles (MV) are small membrane vesicles which are released from cells under physiological and pathological conditions. Microvesicles consists of three subgroups: exosomes (Ex, 40-100nm), microparticles (MP, 0,1µm-1µm) and apoptotic bodies (AB, 1µm-4µm). All three MV subgroups derived from endothelial cells were shown to contain and transfer functional microRNAs (miRs) between cells and thereby regulating vascular protection and regeneration. Within this proposal, we aim to asses how the packaging of selective miRs from cells into MV subgroups is regulated under physiological and pathological conditions. Furthermore, we aim to explore how miRs within MV influence vascular smooth muscle cell (VSMC) biology in vivo and in vitro. Finally, we aim to investigate in detail the role of miR-126 in different vascular injury models to characterize its role in neointimaformation and reendothelialization. With this proposal, we aim to contribute further knowledge about MV-associated miRs in order to probably use miRs within MV in the future as a therapeutic tool.