Role and function of microRNAs within microvesicles in the regulation of vascular biology

微泡内microRNA在血管生物学调节中的作用和功能

• 批准号: 257047998
• 负责人: Professor Dr. Nikos Sebastian Werner
• 金额: --
• 依托单位: Krankenhaus der Barmherzigen Brüder Trier
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/257047998?language=en
• 关键词: Role; function; microRNAs; within; microvesicles

Microvesicles (MV) are small membrane vesicles which are released from cells under physiological and pathological conditions. Microvesicles consists of three subgroups: exosomes (Ex, 40-100nm), microparticles (MP, 0,1µm-1µm) and apoptotic bodies (AB, 1µm-4µm). All three MV subgroups derived from endothelial cells were shown to contain and transfer functional microRNAs (miRs) between cells and thereby regulating vascular protection and regeneration. Within this proposal, we aim to asses how the packaging of selective miRs from cells into MV subgroups is regulated under physiological and pathological conditions. Furthermore, we aim to explore how miRs within MV influence vascular smooth muscle cell (VSMC) biology in vivo and in vitro. Finally, we aim to investigate in detail the role of miR-126 in different vascular injury models to characterize its role in neointimaformation and reendothelialization. With this proposal, we aim to contribute further knowledge about MV-associated miRs in order to probably use miRs within MV in the future as a therapeutic tool.

微囊泡（MV）是在生理和病理条件下从细胞中释放出来的小膜泡。微泡由三个亚群组成：外泌体（Ex, 40-100nm），微粒（MP, 0,1µm-1µm）和凋亡小体（AB, 1µm-4µm）。来源于内皮细胞的所有三个MV亚群都含有并在细胞之间传递功能性microrna (miRs)，从而调节血管保护和再生。在本提案中，我们的目标是评估在生理和病理条件下如何调节选择性miRs从细胞包装成MV亚群。此外，我们的目标是探索MV中的miRs如何在体内和体外影响血管平滑肌细胞（VSMC）生物学。最后，我们的目标是详细研究miR-126在不同血管损伤模型中的作用，以表征其在新信息和再内皮化中的作用。通过这一建议，我们的目标是进一步了解MV相关的miRs，以便将来可能将miRs作为MV的治疗工具。